Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital
Background: The evident multidrug resistance (MDR) associated with Helicobacter pylori (H. pylori) is a serious public health problem. Multidrug resistance was defined as resistance to at least one antimicrobial agent in three or even more antimicrobial classes. The hefA gene encodes an active efflu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Zagazig University, Faculty of Medicine
2023-05-01
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| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_291268_3f7f56f1b14d00a74d62d89b12588679.pdf |
| _version_ | 1797827823096823808 |
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| author | kareman Eshra Ibrahim amer Radwa El Sharaby shimaa El Sharawy Radwa Eissa |
| author_facet | kareman Eshra Ibrahim amer Radwa El Sharaby shimaa El Sharawy Radwa Eissa |
| author_sort | kareman Eshra |
| collection | DOAJ |
| description | Background: The evident multidrug resistance (MDR) associated with Helicobacter pylori (H. pylori) is a serious public health problem. Multidrug resistance was defined as resistance to at least one antimicrobial agent in three or even more antimicrobial classes. The hefA gene encodes an active efflux process against antimicrobials, and its expression in H. pylori may contribute to the upsurge of resistant strains. Aim of the study: Detection of MDR H. pylori among endoscopic isolates and examining the role of hefA gene expression in the occurrence of MDR H. pylori. Methods: Our research involved 40 H. pylori endoscopic isolates using conventional microbiological methods. We identified MDR in H. pylori isolates using E testing procedures for metronidazole, ciprofloxacin, amoxicillin as well as clarithromycin. We detected the hefA gene expression among the isolated H. pylori by real-time PCR. Results: out of 40 isolates of H. pylori, 13 (32.5%) were MDR and 27 (67.5%) were not MDR. We found hefA gene in MDR H. pylori isolates with total (mean ± SD) equal to 7.055385±2.591111, but in non-MDR H. pylori isolates were with total (mean ± SD) equal to 2.591111±0.720189. There was a statistically significant difference in hefA expression levels among both MDR H. pylori isolated strains and non-MDR ones; the p value was 0.001. Conclusion: The hefA gene expression in H. pylori plays an important role in the emergence of MDR H. pylori strains as one of the genes encoding for efflux pump mechanisms. |
| first_indexed | 2024-04-09T12:54:32Z |
| format | Article |
| id | doaj.art-264c3ff174514209ab2f5aa2570c6351 |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-09T12:54:32Z |
| publishDate | 2023-05-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-264c3ff174514209ab2f5aa2570c63512023-05-13T20:41:24ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402023-05-014251452110.21608/mid.2023.191944.1461291268Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospitalkareman Eshra0Ibrahim amer1Radwa El Sharaby2shimaa El Sharawy3Radwa Eissa4medical microbiology and immunology , faculty of medicine, Tanta universityHepatology,gastroentrology&infectious diseases departmentclinical pathology department , Faculty of medicine, Tanta UniversityTropical medicine and infectious diseases Tanta Universitymedical microbiology and immunology department , faculty of medicine , Tanta universityBackground: The evident multidrug resistance (MDR) associated with Helicobacter pylori (H. pylori) is a serious public health problem. Multidrug resistance was defined as resistance to at least one antimicrobial agent in three or even more antimicrobial classes. The hefA gene encodes an active efflux process against antimicrobials, and its expression in H. pylori may contribute to the upsurge of resistant strains. Aim of the study: Detection of MDR H. pylori among endoscopic isolates and examining the role of hefA gene expression in the occurrence of MDR H. pylori. Methods: Our research involved 40 H. pylori endoscopic isolates using conventional microbiological methods. We identified MDR in H. pylori isolates using E testing procedures for metronidazole, ciprofloxacin, amoxicillin as well as clarithromycin. We detected the hefA gene expression among the isolated H. pylori by real-time PCR. Results: out of 40 isolates of H. pylori, 13 (32.5%) were MDR and 27 (67.5%) were not MDR. We found hefA gene in MDR H. pylori isolates with total (mean ± SD) equal to 7.055385±2.591111, but in non-MDR H. pylori isolates were with total (mean ± SD) equal to 2.591111±0.720189. There was a statistically significant difference in hefA expression levels among both MDR H. pylori isolated strains and non-MDR ones; the p value was 0.001. Conclusion: The hefA gene expression in H. pylori plays an important role in the emergence of MDR H. pylori strains as one of the genes encoding for efflux pump mechanisms.https://mid.journals.ekb.eg/article_291268_3f7f56f1b14d00a74d62d89b12588679.pdfmdrh. pylorihefa geneefflux pumpreal time pcr |
| spellingShingle | kareman Eshra Ibrahim amer Radwa El Sharaby shimaa El Sharawy Radwa Eissa Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital Microbes and Infectious Diseases mdr h. pylori hefa gene efflux pump real time pcr |
| title | Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital |
| title_full | Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital |
| title_fullStr | Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital |
| title_full_unstemmed | Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital |
| title_short | Detection of hefA gene in multidrug resistant Helicobacter pylori at Tanta University Hospital |
| title_sort | detection of hefa gene in multidrug resistant helicobacter pylori at tanta university hospital |
| topic | mdr h. pylori hefa gene efflux pump real time pcr |
| url | https://mid.journals.ekb.eg/article_291268_3f7f56f1b14d00a74d62d89b12588679.pdf |
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