Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway
Microcystin-LR (MC-LR), the toxic substance of cyanobacteria secondary metabolism, widely exists in water environments and poses great risks to living organisms. Some toxicological assessments of MC-LR have performed at physiological and biochemical levels. However, plenty of blanks about the potent...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322003682 |
| _version_ | 1818006602172596224 |
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| author | Yuning Zhang Jiancao Gao Zhijuan Nie Haojun Zhu Jinliang Du Liping Cao Nailin Shao Yi Sun Shengyan Su Gangchun Xu Pao Xu |
| author_facet | Yuning Zhang Jiancao Gao Zhijuan Nie Haojun Zhu Jinliang Du Liping Cao Nailin Shao Yi Sun Shengyan Su Gangchun Xu Pao Xu |
| author_sort | Yuning Zhang |
| collection | DOAJ |
| description | Microcystin-LR (MC-LR), the toxic substance of cyanobacteria secondary metabolism, widely exists in water environments and poses great risks to living organisms. Some toxicological assessments of MC-LR have performed at physiological and biochemical levels. However, plenty of blanks about the potential mechanism in aquatic crustacean remains. In this study, we firstly assessed the exposure toxicity of MC-LR to juvenile E. sinensis and clarified that the 96 h LD50 of MC-LR was 73.23 μg/kg. Then, hepatopancreas transcriptome profiles of MC-LR stressed crabs were constructed at 6 h post-injection and 37 differential expressed genes (DEGs) were identified. These DEGs were enriched in cytoskeleton, peroxisome and apoptosis pathways. To further reveal the toxicity of MC-LR, oxidative stress parameters (SOD, CAT, GSH-px and MDA), apoptosis genes (caspase 3, bcl-2 and bax) and apoptotic cells were detected. Significant accumulated MDA and rise-fall enzyme activities verified the oxidative stress caused by MC-LR. It is noteworthy that quantitative real-time PCR and TUNEL assay indicated that MC-LR stress-induced apoptosis via the mitochondrial pathway. Interestingly, activator protein-1 may play a crucial role in mediating the hepatotoxicity of MC-LR by regulating apoptosis and oxidative stress. Taken together, our study investigated the toxic effects and the potential molecular mechanisms of MC-LR on juvenile E. sinensis. It provided useful data for exploring the toxicity of MC-LR to aquatic crustaceans at molecular levels. |
| first_indexed | 2024-04-14T05:03:25Z |
| format | Article |
| id | doaj.art-264d017c2fcc448ab81913b30ea2cf06 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-14T05:03:25Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-264d017c2fcc448ab81913b30ea2cf062022-12-22T02:10:50ZengElsevierEcotoxicology and Environmental Safety0147-65132022-06-01238113528Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathwayYuning Zhang0Jiancao Gao1Zhijuan Nie2Haojun Zhu3Jinliang Du4Liping Cao5Nailin Shao6Yi Sun7Shengyan Su8Gangchun Xu9Pao Xu10Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Corresponding authors at: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Corresponding authors at: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaMicrocystin-LR (MC-LR), the toxic substance of cyanobacteria secondary metabolism, widely exists in water environments and poses great risks to living organisms. Some toxicological assessments of MC-LR have performed at physiological and biochemical levels. However, plenty of blanks about the potential mechanism in aquatic crustacean remains. In this study, we firstly assessed the exposure toxicity of MC-LR to juvenile E. sinensis and clarified that the 96 h LD50 of MC-LR was 73.23 μg/kg. Then, hepatopancreas transcriptome profiles of MC-LR stressed crabs were constructed at 6 h post-injection and 37 differential expressed genes (DEGs) were identified. These DEGs were enriched in cytoskeleton, peroxisome and apoptosis pathways. To further reveal the toxicity of MC-LR, oxidative stress parameters (SOD, CAT, GSH-px and MDA), apoptosis genes (caspase 3, bcl-2 and bax) and apoptotic cells were detected. Significant accumulated MDA and rise-fall enzyme activities verified the oxidative stress caused by MC-LR. It is noteworthy that quantitative real-time PCR and TUNEL assay indicated that MC-LR stress-induced apoptosis via the mitochondrial pathway. Interestingly, activator protein-1 may play a crucial role in mediating the hepatotoxicity of MC-LR by regulating apoptosis and oxidative stress. Taken together, our study investigated the toxic effects and the potential molecular mechanisms of MC-LR on juvenile E. sinensis. It provided useful data for exploring the toxicity of MC-LR to aquatic crustaceans at molecular levels.http://www.sciencedirect.com/science/article/pii/S0147651322003682Microcystin-LREriocheir sinensisHepatopancreas injuryApoptosisAP-1 |
| spellingShingle | Yuning Zhang Jiancao Gao Zhijuan Nie Haojun Zhu Jinliang Du Liping Cao Nailin Shao Yi Sun Shengyan Su Gangchun Xu Pao Xu Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway Ecotoxicology and Environmental Safety Microcystin-LR Eriocheir sinensis Hepatopancreas injury Apoptosis AP-1 |
| title | Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway |
| title_full | Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway |
| title_fullStr | Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway |
| title_full_unstemmed | Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway |
| title_short | Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway |
| title_sort | microcystin lr induces apoptosis in juvenile eriocheir sinensis via the mitochondrial pathway |
| topic | Microcystin-LR Eriocheir sinensis Hepatopancreas injury Apoptosis AP-1 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322003682 |
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