miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten
Objective To study the effect of miR-499a-5p on injury induced by chemical hypoxia of pheochromocytoma cell(PC12). Methods PC12 cells were divided into control group, hypoxia group (treated with CoCl2 for 24 h), hypoxia+miR-NC or miR-mimic groups. Cell viability was detected by MTT assay; the expr...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-12-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1873.pdf |
| _version_ | 1797365836326895616 |
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| author | ZHANG Li-yang, SUN Jun, CHEN Di |
| author_facet | ZHANG Li-yang, SUN Jun, CHEN Di |
| author_sort | ZHANG Li-yang, SUN Jun, CHEN Di |
| collection | DOAJ |
| description | Objective To study the effect of miR-499a-5p on injury induced by chemical hypoxia of pheochromocytoma cell(PC12). Methods PC12 cells were divided into control group, hypoxia group (treated with CoCl2 for 24 h), hypoxia+miR-NC or miR-mimic groups. Cell viability was detected by MTT assay; the expression of miR-499a-5p was detected by RT-qPCR; lactate dehydrogenase (LDH) activity was detected by kit; apoptosis was detected by flow cytometry; the expression of phosphatase and tensin homolog (PTEN) protein was detected by Western blot. The luciferase reporter gene was used to detect whether Pten was a direct target of miR-499a-5p. Results Compared with control group, the cell viability and miR-499a-5p expression decreased (P＜0.01 or P＜0.001), LDH activity and apoptosis increased (P＜0.01 or P＜0.001) in hypoxia group. Over-expression of miR-499a-5p enhanced survival rate and inhibited apoptosis of PC12 cells under hypoxia (P＜0.05 or P＜0.01). Pten was a direct target of miR-499a-5p. Conclusions miR-499a-5p alleviates hypoxic injury of PC12 cells through targeting at Pten. |
| first_indexed | 2024-03-08T16:54:39Z |
| format | Article |
| id | doaj.art-264d695ba68941c5a4f0ce09f597ebb3 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:39Z |
| publishDate | 2022-12-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-264d695ba68941c5a4f0ce09f597ebb32024-01-05T02:36:47ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-12-0142121873187810.16352/j.issn.1001-6325.2022.12.1873miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at PtenZHANG Li-yang, SUN Jun, CHEN Di0Department of Neurology, Nanyang Central Hospital, Nanyang 473000, ChinaObjective To study the effect of miR-499a-5p on injury induced by chemical hypoxia of pheochromocytoma cell(PC12). Methods PC12 cells were divided into control group, hypoxia group (treated with CoCl2 for 24 h), hypoxia+miR-NC or miR-mimic groups. Cell viability was detected by MTT assay; the expression of miR-499a-5p was detected by RT-qPCR; lactate dehydrogenase (LDH) activity was detected by kit; apoptosis was detected by flow cytometry; the expression of phosphatase and tensin homolog (PTEN) protein was detected by Western blot. The luciferase reporter gene was used to detect whether Pten was a direct target of miR-499a-5p. Results Compared with control group, the cell viability and miR-499a-5p expression decreased (P＜0.01 or P＜0.001), LDH activity and apoptosis increased (P＜0.01 or P＜0.001) in hypoxia group. Over-expression of miR-499a-5p enhanced survival rate and inhibited apoptosis of PC12 cells under hypoxia (P＜0.05 or P＜0.01). Pten was a direct target of miR-499a-5p. Conclusions miR-499a-5p alleviates hypoxic injury of PC12 cells through targeting at Pten.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1873.pdfmir-499a-5p|phosphatase and tensin homolog|pc12 cell|hypoxic injury |
| spellingShingle | ZHANG Li-yang, SUN Jun, CHEN Di miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten Jichu yixue yu linchuang mir-499a-5p|phosphatase and tensin homolog|pc12 cell|hypoxic injury |
| title | miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten |
| title_full | miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten |
| title_fullStr | miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten |
| title_full_unstemmed | miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten |
| title_short | miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten |
| title_sort | mir 499a 5p alleviates hypoxic damage of pheochromocytoma cells through targeting at pten |
| topic | mir-499a-5p|phosphatase and tensin homolog|pc12 cell|hypoxic injury |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-12-1873.pdf |
| work_keys_str_mv | AT zhangliyangsunjunchendi mir499a5palleviateshypoxicdamageofpheochromocytomacellsthroughtargetingatpten |