The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice
Abstract Background: Stress is a mental or emotional disturbance that occurs in response to external stimuli and can also appear during pregnancy. The aim of this study is to investigate the effect of maternal stress during pregnancy on the cerebellar structure changes and seizure threshold of thei...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2015-09-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-3423-en.pdf |
| _version_ | 1811277298258673664 |
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| author | Sahar Parsaee Homa Mohseni Kochesfehani Gholamreza Kaka Homayoon Sadraie Mojtaba Kahali |
| author_facet | Sahar Parsaee Homa Mohseni Kochesfehani Gholamreza Kaka Homayoon Sadraie Mojtaba Kahali |
| author_sort | Sahar Parsaee |
| collection | DOAJ |
| description | Abstract
Background: Stress is a mental or emotional disturbance that occurs in response to external stimuli and can also appear during pregnancy. The aim of this study is to investigate the effect of maternal stress during pregnancy on the cerebellar structure changes and seizure threshold of their offspring.
Materials and Methods: In this experimental study, 20 pregnant female rats were divided into two groups: 1) Non stress group, and 2) Stress group which were under immobilization stress one hour for 14 days. The seizure threshold test in offspring was performed by injecting Pantilen tetrazol drug (PTZ)(n=8). To investigate the cerebellum development, the offspring were divided into three groups. Control group: mothers did not any stress and offspring did not receive PTZ(n=4). Sham group: mothers did not stress but the offspring had received PTZ(n=4). Experimental group: mothers did stress and offispring did receive PTZ(n=4). After the section of cerebellum, the thickness of cerebellum layers and the number of cells in each layer were evaluated.
Results: The mean of seizure threshold in the offspring whose mothers were under the stress of pregnancy significantly increased compared to children whose mothers no received stress (p<0.001). In the other side, mean number of purkinje cells in the experimental group significantly decreased compared with the other groups (p<0.001). No significant differences were found in the mean of granular and molecular layers thickness of cerebellum in the experimental group when compared with the other groups(p<0.05). However, mean cellular density in the granular layer of cerebellum in the experimental group significantly decreased compared to other groups (p<0.001).
Conclusion: Stress during pregnancy increased the seizure threshold in offspring and caused some developmental and structural disorders in the cerebellar rat offspring. |
| first_indexed | 2024-04-13T00:14:23Z |
| format | Article |
| id | doaj.art-2651f4ad0f284c448aac517d9ab3b41b |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-04-13T00:14:23Z |
| publishDate | 2015-09-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-2651f4ad0f284c448aac517d9ab3b41b2022-12-22T03:10:59ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2015-09-011861726The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI MiceSahar Parsaee0Homa Mohseni Kochesfehani1Gholamreza Kaka2Homayoon Sadraie3Mojtaba Kahali4Department of Animal Sciences, Kharazmi University, Tehran, Iran.Department of Biology, Kharazmi University, Tehran, Iran.Department of Anatomy, Baqiyatallah University of Medical Sciences, Tehran, Iran.Neurosciences Research center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Neurosciences Research center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Abstract Background: Stress is a mental or emotional disturbance that occurs in response to external stimuli and can also appear during pregnancy. The aim of this study is to investigate the effect of maternal stress during pregnancy on the cerebellar structure changes and seizure threshold of their offspring. Materials and Methods: In this experimental study, 20 pregnant female rats were divided into two groups: 1) Non stress group, and 2) Stress group which were under immobilization stress one hour for 14 days. The seizure threshold test in offspring was performed by injecting Pantilen tetrazol drug (PTZ)(n=8). To investigate the cerebellum development, the offspring were divided into three groups. Control group: mothers did not any stress and offspring did not receive PTZ(n=4). Sham group: mothers did not stress but the offspring had received PTZ(n=4). Experimental group: mothers did stress and offispring did receive PTZ(n=4). After the section of cerebellum, the thickness of cerebellum layers and the number of cells in each layer were evaluated. Results: The mean of seizure threshold in the offspring whose mothers were under the stress of pregnancy significantly increased compared to children whose mothers no received stress (p<0.001). In the other side, mean number of purkinje cells in the experimental group significantly decreased compared with the other groups (p<0.001). No significant differences were found in the mean of granular and molecular layers thickness of cerebellum in the experimental group when compared with the other groups(p<0.05). However, mean cellular density in the granular layer of cerebellum in the experimental group significantly decreased compared to other groups (p<0.001). Conclusion: Stress during pregnancy increased the seizure threshold in offspring and caused some developmental and structural disorders in the cerebellar rat offspring.http://amuj.arakmu.ac.ir/article-1-3423-en.pdfCerebellumPentylenetetrazoleSeizureStress |
| spellingShingle | Sahar Parsaee Homa Mohseni Kochesfehani Gholamreza Kaka Homayoon Sadraie Mojtaba Kahali The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Cerebellum Pentylenetetrazole Seizure Stress |
| title | The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice |
| title_full | The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice |
| title_fullStr | The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice |
| title_full_unstemmed | The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice |
| title_short | The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice |
| title_sort | effect of prenatal stress on seizure threshold and histomorphometrical changes on cerebellar cortex in nmri mice |
| topic | Cerebellum Pentylenetetrazole Seizure Stress |
| url | http://amuj.arakmu.ac.ir/article-1-3423-en.pdf |
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