Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria
Background and Aims: Staphylococcus aureus is one of the most important pathogens of public health concern and a leading cause of nosocomial infections. In this study, we evaluated the effect of routinely used disinfectants in hospitals for surface decontamination on biofilm-forming S. aureus. Ma...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2023-12-01
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| Series: | Microbiologia Medica |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/mm/article/view/11445 |
| _version_ | 1797404625585831936 |
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| author | Utibeima Udo Essiet Abraham Ajayi Adeyemi Isaac Adeleye Stella Ifeanyi Smith |
| author_facet | Utibeima Udo Essiet Abraham Ajayi Adeyemi Isaac Adeleye Stella Ifeanyi Smith |
| author_sort | Utibeima Udo Essiet |
| collection | DOAJ |
| description |
Background and Aims: Staphylococcus aureus is one of the most important pathogens of public health concern and a leading cause of nosocomial infections. In this study, we evaluated the effect of routinely used disinfectants in hospitals for surface decontamination on biofilm-forming S. aureus.
Materials and Methods: forty-eight S. aureus isolates were phenotypically evaluated for biofilm formation using the Tissue Culture Plate (TCP) technique. Effect of disinfectants (Dettol®, Izal®, Jik® and Savlon®) on biofilm was tested and time-kill kinetics evaluated. PCR was used to confirm the identity of S. aureus using species-specific primers.
Results: biofilm formation assay revealed that 15 (31.2%) of the isolates formed biofilm with 7 (14.5%) and 8 (16.6%) considered as strong and moderate biofilm formers, respectively. Biofilm formation was time-dependent (p<0.0001). Jik® was significantly effective (p<0.0001) as it disrupted biofilm formed in all 15 (100%) isolates, followed by Izal® 13 (86.6%), Savlon® 11 (73.3%) and Dettol® 9 (60%). Time-kill kinetics of the four disinfectants revealed Dettol®, Jik® and Savlon® achieved total (100%), (7 log10) lethality against isolates within 1 h contact time while Izal® attained complete lethality at 6 h contact time.
Conclusions: of the four disinfectants evaluated Jik®, a chlorine- based formulation, was more effective in destroying biofilmforming S. aureus. The need to use effective disinfectants in sanitization is imperative to facilitate the control and prevention of hospital and community-acquired infections.
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| first_indexed | 2024-03-09T02:57:45Z |
| format | Article |
| id | doaj.art-2652f2e41bc74baea6001e74783a0aca |
| institution | Directory Open Access Journal |
| issn | 2280-6423 |
| language | English |
| last_indexed | 2024-03-09T02:57:45Z |
| publishDate | 2023-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj.art-2652f2e41bc74baea6001e74783a0aca2023-12-04T23:06:03ZengPAGEPress PublicationsMicrobiologia Medica2280-64232023-12-0138210.4081/mm.2023.11445Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, NigeriaUtibeima Udo Essiet0Abraham Ajayi1Adeyemi Isaac Adeleye2Stella Ifeanyi Smith3Department of Microbiology, University of Lagos, Akoka, Lagos StateMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research (NIMR) Yaba, Lagos StateDepartment of Microbiology, University of Lagos, Akoka, Lagos StateMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research (NIMR) Yaba, Lagos State; Department of Biological Sciences, Mountain Top University, Ogun State Background and Aims: Staphylococcus aureus is one of the most important pathogens of public health concern and a leading cause of nosocomial infections. In this study, we evaluated the effect of routinely used disinfectants in hospitals for surface decontamination on biofilm-forming S. aureus. Materials and Methods: forty-eight S. aureus isolates were phenotypically evaluated for biofilm formation using the Tissue Culture Plate (TCP) technique. Effect of disinfectants (Dettol®, Izal®, Jik® and Savlon®) on biofilm was tested and time-kill kinetics evaluated. PCR was used to confirm the identity of S. aureus using species-specific primers. Results: biofilm formation assay revealed that 15 (31.2%) of the isolates formed biofilm with 7 (14.5%) and 8 (16.6%) considered as strong and moderate biofilm formers, respectively. Biofilm formation was time-dependent (p<0.0001). Jik® was significantly effective (p<0.0001) as it disrupted biofilm formed in all 15 (100%) isolates, followed by Izal® 13 (86.6%), Savlon® 11 (73.3%) and Dettol® 9 (60%). Time-kill kinetics of the four disinfectants revealed Dettol®, Jik® and Savlon® achieved total (100%), (7 log10) lethality against isolates within 1 h contact time while Izal® attained complete lethality at 6 h contact time. Conclusions: of the four disinfectants evaluated Jik®, a chlorine- based formulation, was more effective in destroying biofilmforming S. aureus. The need to use effective disinfectants in sanitization is imperative to facilitate the control and prevention of hospital and community-acquired infections. https://www.pagepressjournals.org/index.php/mm/article/view/11445BiofilmchlorinedisinfectantsStaphylococcus aureus |
| spellingShingle | Utibeima Udo Essiet Abraham Ajayi Adeyemi Isaac Adeleye Stella Ifeanyi Smith Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria Microbiologia Medica Biofilm chlorine disinfectants Staphylococcus aureus |
| title | Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria |
| title_full | Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria |
| title_fullStr | Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria |
| title_full_unstemmed | Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria |
| title_short | Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria |
| title_sort | effect of selected disinfectants on biofilm forming clinical isolates of staphylococcus aureus in lagos state nigeria |
| topic | Biofilm chlorine disinfectants Staphylococcus aureus |
| url | https://www.pagepressjournals.org/index.php/mm/article/view/11445 |
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