Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Polycystic Kidney Disease (<i>PKD</i>) is a disorder that affects the kidneys and other organs, and its major forms are encoded by polycystin-1 (<i>PC1</i>) and polycystin-2 (<i>PC2</i>), as <i>PKD1</i> and <i>PKD2.</i> It is located sandwi...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4868 |
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| author | Se-Hwa Jung Ji-Eun You Soon-Won Choi Kyung-Sun Kang Je-Yeol Cho Jungmook Lyu Pyung-Hwan Kim |
| author_facet | Se-Hwa Jung Ji-Eun You Soon-Won Choi Kyung-Sun Kang Je-Yeol Cho Jungmook Lyu Pyung-Hwan Kim |
| author_sort | Se-Hwa Jung |
| collection | DOAJ |
| description | Polycystic Kidney Disease (<i>PKD</i>) is a disorder that affects the kidneys and other organs, and its major forms are encoded by polycystin-1 (<i>PC1</i>) and polycystin-2 (<i>PC2</i>), as <i>PKD1</i> and <i>PKD2.</i> It is located sandwiched inside and outside cell membranes and interacts with other cells. This protein is most active in kidney cells before birth, and <i>PC1</i> and <i>PC2</i> work together to help regulate cell proliferation, cell migration, and interactions with other cells. The molecular relationship and the function between <i>PKD1</i> and cancer is well known, such as increased or decreased cell proliferation and promoting or suppressing cell migration depending on the cancer cell type specifically. However, its function in stem cells has not been revealed. Therefore, in this study, we investigated the biological function of <i>PC1</i> and umbilical cord blood-derived mesenchymal stem cell (UCB-MSC). Furthermore, we assessed how it affects cell migration, proliferation, and the viability of cells when expressed in the <i>PKD1</i> gene. In addition, we confirmed in an ex vivo artificial tooth model generated by the three-dimension printing technique that the ability to differentiate into osteocytes improved according to the expression level of the stemness markers when <i>PKD1</i> was expressed. This study is the first report to examine the biological function of <i>PKD1</i> in UCB-MSC. This gene may be capable of enhancing differentiation ability and maintaining long-term stemness for the therapeutic use of stem cells. |
| first_indexed | 2024-03-10T11:42:08Z |
| format | Article |
| id | doaj.art-2654b4c632024ed3be530dac2bef8e3f |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:42:08Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2654b4c632024ed3be530dac2bef8e3f2023-11-21T18:21:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01229486810.3390/ijms22094868Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem CellsSe-Hwa Jung0Ji-Eun You1Soon-Won Choi2Kyung-Sun Kang3Je-Yeol Cho4Jungmook Lyu5Pyung-Hwan Kim6Department of Biomedical Laboratory Science, Konyang University, Daejeon 35365, KoreaDepartment of Biomedical Laboratory Science, Konyang University, Daejeon 35365, KoreaAdult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaAdult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul 151-742, KoreaMyung-Gok Eye Research Institute, Department of Medical Science, Konyang University, Daejeon 320-832, KoreaDepartment of Biomedical Laboratory Science, Konyang University, Daejeon 35365, KoreaPolycystic Kidney Disease (<i>PKD</i>) is a disorder that affects the kidneys and other organs, and its major forms are encoded by polycystin-1 (<i>PC1</i>) and polycystin-2 (<i>PC2</i>), as <i>PKD1</i> and <i>PKD2.</i> It is located sandwiched inside and outside cell membranes and interacts with other cells. This protein is most active in kidney cells before birth, and <i>PC1</i> and <i>PC2</i> work together to help regulate cell proliferation, cell migration, and interactions with other cells. The molecular relationship and the function between <i>PKD1</i> and cancer is well known, such as increased or decreased cell proliferation and promoting or suppressing cell migration depending on the cancer cell type specifically. However, its function in stem cells has not been revealed. Therefore, in this study, we investigated the biological function of <i>PC1</i> and umbilical cord blood-derived mesenchymal stem cell (UCB-MSC). Furthermore, we assessed how it affects cell migration, proliferation, and the viability of cells when expressed in the <i>PKD1</i> gene. In addition, we confirmed in an ex vivo artificial tooth model generated by the three-dimension printing technique that the ability to differentiate into osteocytes improved according to the expression level of the stemness markers when <i>PKD1</i> was expressed. This study is the first report to examine the biological function of <i>PKD1</i> in UCB-MSC. This gene may be capable of enhancing differentiation ability and maintaining long-term stemness for the therapeutic use of stem cells.https://www.mdpi.com/1422-0067/22/9/4868<i>PKD1</i>umbilical cord blood-derived mesenchymal stem cell (UCB-MSC)stemnessosteogenic differentiation |
| spellingShingle | Se-Hwa Jung Ji-Eun You Soon-Won Choi Kyung-Sun Kang Je-Yeol Cho Jungmook Lyu Pyung-Hwan Kim Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells International Journal of Molecular Sciences <i>PKD1</i> umbilical cord blood-derived mesenchymal stem cell (UCB-MSC) stemness osteogenic differentiation |
| title | Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells |
| title_full | Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells |
| title_fullStr | Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells |
| title_short | Polycystin-1 Enhances Stemmness Potential of Umbilical Cord Blood-Derived Mesenchymal Stem Cells |
| title_sort | polycystin 1 enhances stemmness potential of umbilical cord blood derived mesenchymal stem cells |
| topic | <i>PKD1</i> umbilical cord blood-derived mesenchymal stem cell (UCB-MSC) stemness osteogenic differentiation |
| url | https://www.mdpi.com/1422-0067/22/9/4868 |
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