In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches
Lumbar disc herniation (LDH) is the most common reason for low back pain in the working society. New regenerative approaches and novel implants are directed towards the restoration of the disc or its biomechanical properties. Aiming to investigate these new therapies under physiological conditions,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/6/2847 |
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| author | Laura Zengerle Elisabeth Debout Bruno Kluger Lena Zöllner Hans-Joachim Wilke |
| author_facet | Laura Zengerle Elisabeth Debout Bruno Kluger Lena Zöllner Hans-Joachim Wilke |
| author_sort | Laura Zengerle |
| collection | DOAJ |
| description | Lumbar disc herniation (LDH) is the most common reason for low back pain in the working society. New regenerative approaches and novel implants are directed towards the restoration of the disc or its biomechanical properties. Aiming to investigate these new therapies under physiological conditions, in this study, a model for LDH was established by developing a new physiological in vitro test method. In 14 human lumbar motion segments, different daily-life and worst-case activities were simulated successfully by applying a physiological range of motion and axial loading in order to create physiological intradiscal pressure. An LDH could be provoked in 11 of the 14 specimens through vertical and round annular defects of different sizes. Interestingly, the defect and the LDH hardly influenced the biomechanical properties of the disc. For the investigation of regenerative approaches in further experiments, the recommendation based on the results of this study is to create an LDH in non-degenerated motion segments by the application of the new physiological in vitro test method after setting the round annular defects to a size of 4 mm in diameter. |
| first_indexed | 2024-03-10T12:59:44Z |
| format | Article |
| id | doaj.art-2656a1f34a284f468a238b633ea401b3 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T12:59:44Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-2656a1f34a284f468a238b633ea401b32023-11-21T11:35:13ZengMDPI AGApplied Sciences2076-34172021-03-01116284710.3390/app11062847In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair ApproachesLaura Zengerle0Elisabeth Debout1Bruno Kluger2Lena Zöllner3Hans-Joachim Wilke4Institute of Orthopaedic Research and Biomechanics, Ulm University, 89081 Ulm, GermanyInstitute of Orthopaedic Research and Biomechanics, Ulm University, 89081 Ulm, GermanyInstitute for Laser Technologies in Medicine and Metrology, Ulm University, 89081 Ulm, GermanyInstitute of Orthopaedic Research and Biomechanics, Ulm University, 89081 Ulm, GermanyInstitute of Orthopaedic Research and Biomechanics, Ulm University, 89081 Ulm, GermanyLumbar disc herniation (LDH) is the most common reason for low back pain in the working society. New regenerative approaches and novel implants are directed towards the restoration of the disc or its biomechanical properties. Aiming to investigate these new therapies under physiological conditions, in this study, a model for LDH was established by developing a new physiological in vitro test method. In 14 human lumbar motion segments, different daily-life and worst-case activities were simulated successfully by applying a physiological range of motion and axial loading in order to create physiological intradiscal pressure. An LDH could be provoked in 11 of the 14 specimens through vertical and round annular defects of different sizes. Interestingly, the defect and the LDH hardly influenced the biomechanical properties of the disc. For the investigation of regenerative approaches in further experiments, the recommendation based on the results of this study is to create an LDH in non-degenerated motion segments by the application of the new physiological in vitro test method after setting the round annular defects to a size of 4 mm in diameter.https://www.mdpi.com/2076-3417/11/6/2847test methoddynamic testingin vitro(lumbar) disc herniationphysiological activitiesregenerative approaches |
| spellingShingle | Laura Zengerle Elisabeth Debout Bruno Kluger Lena Zöllner Hans-Joachim Wilke In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches Applied Sciences test method dynamic testing in vitro (lumbar) disc herniation physiological activities regenerative approaches |
| title | In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches |
| title_full | In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches |
| title_fullStr | In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches |
| title_full_unstemmed | In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches |
| title_short | In Vitro Model for Lumbar Disc Herniation to Investigate Regenerative Tissue Repair Approaches |
| title_sort | in vitro model for lumbar disc herniation to investigate regenerative tissue repair approaches |
| topic | test method dynamic testing in vitro (lumbar) disc herniation physiological activities regenerative approaches |
| url | https://www.mdpi.com/2076-3417/11/6/2847 |
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