Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification
miRNAs alter significantly throughout pregnancy to support the development of the fetus. However, sensitive detection of miRNA remains a challenge. Herein, a reliable miRNA detection approach integrating self-assembly-triggered signal amplification and CRISPR-Cas12a-system cleavage-based color gener...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-04-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0008 |
| _version_ | 1797825233115152384 |
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| author | Ying Kang Jingjing Zhang Lijuan Zhao Hongli Yan |
| author_facet | Ying Kang Jingjing Zhang Lijuan Zhao Hongli Yan |
| author_sort | Ying Kang |
| collection | DOAJ |
| description | miRNAs alter significantly throughout pregnancy to support the development of the fetus. However, sensitive detection of miRNA remains a challenge. Herein, a reliable miRNA detection approach integrating self-assembly-triggered signal amplification and CRISPR-Cas12a-system cleavage-based color generation is described. The colorimetric approach contains three signal amplification processes. The first signal amplification is formed by the released miRNA in a chain extension process. The produced sequence that is similar to the target miRNA initiates the second signal recycle. Finally, CRISPR-Cas12a-based transcleavage on linker sequences induces the third signal amplification. The method exhibits high sensitivity and a low limit of detection of 254 aM, showing promising prospects in disease diagnosis. |
| first_indexed | 2024-03-13T10:51:00Z |
| format | Article |
| id | doaj.art-265c01338e224dea92f55076792eeac1 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-13T10:51:00Z |
| publishDate | 2023-04-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-265c01338e224dea92f55076792eeac12023-05-17T14:26:27ZengFuture Science LtdBioTechniques0736-62051940-98182023-04-0174417217810.2144/btn-2023-0008Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplificationYing Kang0Jingjing Zhang1Lijuan Zhao2Hongli Yan31Obstetrics Department I, Northwest Women & Children's Hospital, Xi'an, Shaanxi Province, 710061, China1Obstetrics Department I, Northwest Women & Children's Hospital, Xi'an, Shaanxi Province, 710061, China1Obstetrics Department I, Northwest Women & Children's Hospital, Xi'an, Shaanxi Province, 710061, China2Department of Obstetrics & Gynecology, Northwest Women & Children's Hospital, Xi'an, Shaanxi Province, 710061, ChinamiRNAs alter significantly throughout pregnancy to support the development of the fetus. However, sensitive detection of miRNA remains a challenge. Herein, a reliable miRNA detection approach integrating self-assembly-triggered signal amplification and CRISPR-Cas12a-system cleavage-based color generation is described. The colorimetric approach contains three signal amplification processes. The first signal amplification is formed by the released miRNA in a chain extension process. The produced sequence that is similar to the target miRNA initiates the second signal recycle. Finally, CRISPR-Cas12a-based transcleavage on linker sequences induces the third signal amplification. The method exhibits high sensitivity and a low limit of detection of 254 aM, showing promising prospects in disease diagnosis.https://www.future-science.com/doi/10.2144/btn-2023-0008colorimetricCRISPR-Cas12amicroRNA (miRNA)pregnancy |
| spellingShingle | Ying Kang Jingjing Zhang Lijuan Zhao Hongli Yan Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification BioTechniques colorimetric CRISPR-Cas12a microRNA (miRNA) pregnancy |
| title | Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification |
| title_full | Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification |
| title_fullStr | Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification |
| title_full_unstemmed | Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification |
| title_short | Colorimetric miRNA detection based on self-primer-initiated CRISPR-Cas12a-assisted amplification |
| title_sort | colorimetric mirna detection based on self primer initiated crispr cas12a assisted amplification |
| topic | colorimetric CRISPR-Cas12a microRNA (miRNA) pregnancy |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0008 |
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