Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis
"nBackground: The aim of this study was using ELISA for detection of anti-Schistosoma haematobium antibodies in both sera and urine of patients with urinary schistosomiasis. "nMethods: Thirty three sera and urine samples were collected from patients with acute schistosomiasis in Diyala...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-12-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14918.pdf&manuscript_id=14918 |
| _version_ | 1818295346966560768 |
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| author | F Ghaffarifar E Elkawaz |
| author_facet | F Ghaffarifar E Elkawaz |
| author_sort | F Ghaffarifar |
| collection | DOAJ |
| description | "nBackground: The aim of this study was using ELISA for detection of anti-Schistosoma haematobium antibodies in both sera and urine of patients with urinary schistosomiasis. "nMethods: Thirty three sera and urine samples were collected from patients with acute schistosomiasis in Diyala Province, east of Iraq in 2006. Their diseases were confirmed by finding S. haematobium ova in urine examination. Sera and urines of 10 healthy individuals as well as 5 patients with hydatidosis and 5 patients with acute toxoplasmosis were examined as well. Samples were examined for antibody detection by ELISA method. The antigens used in this study were egg and adult antigens. "nResults: All positive samples (sera and urines) showed positivity by using egg antigen whereas the negative control samples were negative; only two samples with hydatidosis were positive with using serum sample whereas with urine sample only one sample was positive. In this study, the best sensitivity and specificity obtained when using urine and adult antigen. "nConclusion: Antibody detection by using urine is a useful, simple, and sensitive method for diagnosis of schistosomiasis. |
| first_indexed | 2024-12-13T03:46:12Z |
| format | Article |
| id | doaj.art-265cec46ad6049cd878e365895ae56c3 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-13T03:46:12Z |
| publishDate | 2009-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-265cec46ad6049cd878e365895ae56c32022-12-22T00:00:49ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2009-12-01445560Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary SchistosomiasisF GhaffarifarE Elkawaz"nBackground: The aim of this study was using ELISA for detection of anti-Schistosoma haematobium antibodies in both sera and urine of patients with urinary schistosomiasis. "nMethods: Thirty three sera and urine samples were collected from patients with acute schistosomiasis in Diyala Province, east of Iraq in 2006. Their diseases were confirmed by finding S. haematobium ova in urine examination. Sera and urines of 10 healthy individuals as well as 5 patients with hydatidosis and 5 patients with acute toxoplasmosis were examined as well. Samples were examined for antibody detection by ELISA method. The antigens used in this study were egg and adult antigens. "nResults: All positive samples (sera and urines) showed positivity by using egg antigen whereas the negative control samples were negative; only two samples with hydatidosis were positive with using serum sample whereas with urine sample only one sample was positive. In this study, the best sensitivity and specificity obtained when using urine and adult antigen. "nConclusion: Antibody detection by using urine is a useful, simple, and sensitive method for diagnosis of schistosomiasis.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14918.pdf&manuscript_id=14918 SchistosomiasisIraqDiagnosisSeraUrineELISA |
| spellingShingle | F Ghaffarifar E Elkawaz Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis Iranian Journal of Parasitology Schistosomiasis Iraq Diagnosis Sera Urine ELISA |
| title | Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis |
| title_full | Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis |
| title_fullStr | Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis |
| title_full_unstemmed | Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis |
| title_short | Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis |
| title_sort | evaluation of schistosoma haematobium adult and egg antigens by elisa in diagnosis of urinary schistosomiasis |
| topic | Schistosomiasis Iraq Diagnosis Sera Urine ELISA |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14918.pdf&manuscript_id=14918 |
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