Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection
Abstract Background Pine wilt disease (PWD) is a destructive disease that endangers pine trees, resulting in the wilting, with yellowing and browning of the needles, and eventually the death of the trees. Previous studies showed that the Avr9/Cf-9 rapidly elicited (PmACRE1) gene was downregulated by...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03929-7 |
| _version_ | 1811211010902589440 |
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| author | Wanfeng Xie Xiaomei Xu Wenjing Qiu Xiaolin Lai Mengxia Liu Feiping Zhang |
| author_facet | Wanfeng Xie Xiaomei Xu Wenjing Qiu Xiaolin Lai Mengxia Liu Feiping Zhang |
| author_sort | Wanfeng Xie |
| collection | DOAJ |
| description | Abstract Background Pine wilt disease (PWD) is a destructive disease that endangers pine trees, resulting in the wilting, with yellowing and browning of the needles, and eventually the death of the trees. Previous studies showed that the Avr9/Cf-9 rapidly elicited (PmACRE1) gene was downregulated by Bursaphelenchus xylophilus infection, suggesting a correlation between PmACRE1 expression and pine tolerance. Here, we used the expression of PmACRE1 in Arabidopsis thaliana to evaluate the role of PmACRE1 in the regulation of host defence against B. xylophilus infection. Results Our results showed that the transformation of PmACRE1 into A. thaliana enhanced plant resistance to the pine wood nematode (PWN); that is, the leaves of the transgenic line remained healthy for a longer period than those of the blank vector group. Ascorbate peroxidase (APX) activity and total phenolic acid and total flavonoid contents were higher in the transgenic line than in the control line. Widely targeted metabolomics analysis of the global secondary metabolites in the transgenic line and the vector control line showed that the contents of 30 compounds were significantly different between these two lines; specifically, the levels of crotaline, neohesperidin, nobiletin, vestitol, and 11 other compounds were significantly increased in the transgenic line. The studies also showed that the ACRE1 protein interacted with serine hydroxymethyltransferase, catalase domain-containing protein, myrosinase, dihydrolipoyl dehydrogenase, ketol-acid reductoisomerase, geranylgeranyl diphosphate reductase, S-adenosylmethionine synthase, glutamine synthetase, and others to comprehensively regulate plant resistance. Conclusions Taken together, these results indicate that PmACRE1 has a potential role in the regulation of plant defence against PWNs. |
| first_indexed | 2024-04-12T05:05:46Z |
| format | Article |
| id | doaj.art-265d116511c54baf8df5a3abc882779e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T05:05:46Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-265d116511c54baf8df5a3abc882779e2022-12-22T03:46:52ZengBMCBMC Plant Biology1471-22292022-11-0122111410.1186/s12870-022-03929-7Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infectionWanfeng Xie0Xiaomei Xu1Wenjing Qiu2Xiaolin Lai3Mengxia Liu4Feiping Zhang5Jinshan College, Fujian Agriculture and Forestry UniversityKey Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry UniversityKey Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry UniversityKey Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry UniversityKey Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry UniversityKey Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry UniversityAbstract Background Pine wilt disease (PWD) is a destructive disease that endangers pine trees, resulting in the wilting, with yellowing and browning of the needles, and eventually the death of the trees. Previous studies showed that the Avr9/Cf-9 rapidly elicited (PmACRE1) gene was downregulated by Bursaphelenchus xylophilus infection, suggesting a correlation between PmACRE1 expression and pine tolerance. Here, we used the expression of PmACRE1 in Arabidopsis thaliana to evaluate the role of PmACRE1 in the regulation of host defence against B. xylophilus infection. Results Our results showed that the transformation of PmACRE1 into A. thaliana enhanced plant resistance to the pine wood nematode (PWN); that is, the leaves of the transgenic line remained healthy for a longer period than those of the blank vector group. Ascorbate peroxidase (APX) activity and total phenolic acid and total flavonoid contents were higher in the transgenic line than in the control line. Widely targeted metabolomics analysis of the global secondary metabolites in the transgenic line and the vector control line showed that the contents of 30 compounds were significantly different between these two lines; specifically, the levels of crotaline, neohesperidin, nobiletin, vestitol, and 11 other compounds were significantly increased in the transgenic line. The studies also showed that the ACRE1 protein interacted with serine hydroxymethyltransferase, catalase domain-containing protein, myrosinase, dihydrolipoyl dehydrogenase, ketol-acid reductoisomerase, geranylgeranyl diphosphate reductase, S-adenosylmethionine synthase, glutamine synthetase, and others to comprehensively regulate plant resistance. Conclusions Taken together, these results indicate that PmACRE1 has a potential role in the regulation of plant defence against PWNs.https://doi.org/10.1186/s12870-022-03929-7Pine wilt diseasePmACRE1MetabolitesDisease resistanceProtein interactions |
| spellingShingle | Wanfeng Xie Xiaomei Xu Wenjing Qiu Xiaolin Lai Mengxia Liu Feiping Zhang Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection BMC Plant Biology Pine wilt disease PmACRE1 Metabolites Disease resistance Protein interactions |
| title | Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection |
| title_full | Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection |
| title_fullStr | Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection |
| title_full_unstemmed | Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection |
| title_short | Expression of PmACRE1 in Arabidopsis thaliana enables host defence against Bursaphelenchus xylophilus infection |
| title_sort | expression of pmacre1 in arabidopsis thaliana enables host defence against bursaphelenchus xylophilus infection |
| topic | Pine wilt disease PmACRE1 Metabolites Disease resistance Protein interactions |
| url | https://doi.org/10.1186/s12870-022-03929-7 |
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