Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay

Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay

This study focuses on three key aspects: (a) crude throat swab samples in a viral transport medium (VTM) as templates for RT-LAMP reactions; (b) a biotinylated DNA probe with enhanced specificity for LFA readouts; and (c) a digital semi-quantification of LFA readouts. Throat swab samples from SARS-C...

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Bibliographic Details
Main Authors: Saloni Agarwal, Mojdeh Hamidizadeh, Frank F. Bier
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Biosensors
Subjects:
RT-LAMP
LFA
NAAT-LFA
semi-quantitative
surveillance-based diagnostics
Online Access:https://www.mdpi.com/2079-6374/13/11/988

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