Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways
The research presents data from a preclinical study on the anti-inflammatory effects of a sodium dichloroacetate and sodium valproate combination (DCA–VPA). The 2-week treatment with a DCA 100 mg/kg/day and VPA 150 mg/kg/day combination solution in drinking water’s effects on the thymus weight, its...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/1999-4923/15/12/2715 |
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| author | Donatas Stakišaitis Linas Kapočius Evelina Kilimaitė Dovydas Gečys Lina Šlekienė Ingrida Balnytė Jolita Palubinskienė Vaiva Lesauskaitė |
| author_facet | Donatas Stakišaitis Linas Kapočius Evelina Kilimaitė Dovydas Gečys Lina Šlekienė Ingrida Balnytė Jolita Palubinskienė Vaiva Lesauskaitė |
| author_sort | Donatas Stakišaitis |
| collection | DOAJ |
| description | The research presents data from a preclinical study on the anti-inflammatory effects of a sodium dichloroacetate and sodium valproate combination (DCA–VPA). The 2-week treatment with a DCA 100 mg/kg/day and VPA 150 mg/kg/day combination solution in drinking water’s effects on the thymus weight, its cortex/medulla ratio, Hassall’s corpuscles (HCs) number in the thymus medulla, and the expression of inflammatory and immune-response-related genes in thymocytes of male Balb/c mice were studied. Two groups of mice aged 6–7 weeks were investigated: a control (<i>n</i> = 12) and a DCA–VPA-treated group (<i>n</i> = 12). The treatment did not affect the body weight gain (<i>p</i> > 0.05), the thymus weight (<i>p</i> > 0.05), the cortical/medulla ratio (<i>p</i> > 0.05), or the number of HCs (<i>p</i> > 0.05). Treatment significantly increased the Slc5a8 gene expression by 2.1-fold (<i>p</i> < 0.05). Gene sequence analysis revealed a significant effect on the expression of inflammation-related genes in thymocytes by significantly altering the expression of several genes related to the cytokine activity pathway, the inflammatory response pathway, and the Il17 signaling pathway in thymocytes. Data suggest that DCA–VPA exerts an anti-inflammatory effect by inhibiting the inflammatory mechanisms in the mouse thymocytes. |
| first_indexed | 2024-03-08T20:26:44Z |
| format | Article |
| id | doaj.art-2661ae930afe4ce98ad80ff1c2aed14f |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T20:26:44Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2661ae930afe4ce98ad80ff1c2aed14f2023-12-22T14:32:06ZengMDPI AGPharmaceutics1999-49232023-11-011512271510.3390/pharmaceutics15122715Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation PathwaysDonatas Stakišaitis0Linas Kapočius1Evelina Kilimaitė2Dovydas Gečys3Lina Šlekienė4Ingrida Balnytė5Jolita Palubinskienė6Vaiva Lesauskaitė7Department of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaLaboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave., 50161 Kaunas, LithuaniaDepartment of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaLaboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave., 50161 Kaunas, LithuaniaThe research presents data from a preclinical study on the anti-inflammatory effects of a sodium dichloroacetate and sodium valproate combination (DCA–VPA). The 2-week treatment with a DCA 100 mg/kg/day and VPA 150 mg/kg/day combination solution in drinking water’s effects on the thymus weight, its cortex/medulla ratio, Hassall’s corpuscles (HCs) number in the thymus medulla, and the expression of inflammatory and immune-response-related genes in thymocytes of male Balb/c mice were studied. Two groups of mice aged 6–7 weeks were investigated: a control (<i>n</i> = 12) and a DCA–VPA-treated group (<i>n</i> = 12). The treatment did not affect the body weight gain (<i>p</i> > 0.05), the thymus weight (<i>p</i> > 0.05), the cortical/medulla ratio (<i>p</i> > 0.05), or the number of HCs (<i>p</i> > 0.05). Treatment significantly increased the Slc5a8 gene expression by 2.1-fold (<i>p</i> < 0.05). Gene sequence analysis revealed a significant effect on the expression of inflammation-related genes in thymocytes by significantly altering the expression of several genes related to the cytokine activity pathway, the inflammatory response pathway, and the Il17 signaling pathway in thymocytes. Data suggest that DCA–VPA exerts an anti-inflammatory effect by inhibiting the inflammatory mechanisms in the mouse thymocytes.https://www.mdpi.com/1999-4923/15/12/2715inflammationviral infectionbacterial infectionthymusthymocytesinvestigational medicinal preparation |
| spellingShingle | Donatas Stakišaitis Linas Kapočius Evelina Kilimaitė Dovydas Gečys Lina Šlekienė Ingrida Balnytė Jolita Palubinskienė Vaiva Lesauskaitė Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways Pharmaceutics inflammation viral infection bacterial infection thymus thymocytes investigational medicinal preparation |
| title | Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways |
| title_full | Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways |
| title_fullStr | Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways |
| title_full_unstemmed | Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways |
| title_short | Preclinical Study in Mouse Thymus and Thymocytes: Effects of Treatment with a Combination of Sodium Dichloroacetate and Sodium Valproate on Infectious Inflammation Pathways |
| title_sort | preclinical study in mouse thymus and thymocytes effects of treatment with a combination of sodium dichloroacetate and sodium valproate on infectious inflammation pathways |
| topic | inflammation viral infection bacterial infection thymus thymocytes investigational medicinal preparation |
| url | https://www.mdpi.com/1999-4923/15/12/2715 |
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