Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves
In plants, prolonged drought induces oxidative stress, leading to a loss of reducing potential in redox components. Abscisic acid (ABA) is a representative hormonal signal regulating stress responses. This study aimed to investigate the physiological significance of dimethylthiourea (DMTU, an H<s...
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/11/2283 |
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| author | Bok-Rye Lee Van Hien La Sang-Hyun Park Md Al Mamun Dong-Won Bae Tae-Hwan Kim |
| author_facet | Bok-Rye Lee Van Hien La Sang-Hyun Park Md Al Mamun Dong-Won Bae Tae-Hwan Kim |
| author_sort | Bok-Rye Lee |
| collection | DOAJ |
| description | In plants, prolonged drought induces oxidative stress, leading to a loss of reducing potential in redox components. Abscisic acid (ABA) is a representative hormonal signal regulating stress responses. This study aimed to investigate the physiological significance of dimethylthiourea (DMTU, an H<sub>2</sub>O<sub>2</sub> scavenger) in the hormonal regulation of the antioxidant system and redox control in rapeseed (<i>Brassica napus</i> L.) leaves under drought stress. Drought treatment for 10 days provoked oxidative stress, as evidenced by the increase in O<sub>2</sub><sup>•−</sup> and H<sub>2</sub>O<sub>2</sub> concentrations, and lipid peroxidation levels, and a decrease in leaf water potential. Drought-induced oxidative responses were significantly alleviated by DMTU treatment. The accumulation of O<sub>2</sub><sup>•−</sup> and H<sub>2</sub>O<sub>2</sub> in drought-treated plants coincided with the enhanced expression of the <i>NADPH oxidase</i> and <i>Cu/Zn-SOD</i> genes, leading to an up-regulation in oxidative signal-inducible 1 (<i>OXI1</i>) and mitogen-activated protein kinase 6 (<i>MAPK6</i>), with a concomitant increase in ABA levels and the up-regulation of ABA-related genes. DMTU treatment under drought largely suppressed the drought-responsive up-regulation of these genes by depressing ABA responses through an antagonistic interaction with salicylic acid (SA). DMTU treatment also alleviated the drought-induced loss of reducing potential in GSH- and NADPH-based redox by the enhanced expression of glutathione reductase 1 (<i>GR1</i>) and up-regulation of oxidoreductase genes (<i>TRXh5</i> and <i>GRXC9</i>). These results indicate that DMTU effectively alleviates drought-induced oxidative responses by suppressing ABA-mediated oxidative burst signaling in an antagonistic regulation of SA. |
| first_indexed | 2024-03-09T18:30:59Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T18:30:59Z |
| publishDate | 2022-11-01 |
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| series | Antioxidants |
| spelling | doaj.art-26652b656af746c79dc1af317c7870162023-11-24T07:32:41ZengMDPI AGAntioxidants2076-39212022-11-011111228310.3390/antiox11112283Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> LeavesBok-Rye Lee0Van Hien La1Sang-Hyun Park2Md Al Mamun3Dong-Won Bae4Tae-Hwan Kim5Grassland Science Laboratory, Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, Republic of KoreaGrassland Science Laboratory, Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, Republic of KoreaGrassland Science Laboratory, Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, Republic of KoreaGrassland Science Laboratory, Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, Republic of KoreaCentral Instrument Facility, Gyeongsang National University, Jinju 52828, Republic of KoreaGrassland Science Laboratory, Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, Republic of KoreaIn plants, prolonged drought induces oxidative stress, leading to a loss of reducing potential in redox components. Abscisic acid (ABA) is a representative hormonal signal regulating stress responses. This study aimed to investigate the physiological significance of dimethylthiourea (DMTU, an H<sub>2</sub>O<sub>2</sub> scavenger) in the hormonal regulation of the antioxidant system and redox control in rapeseed (<i>Brassica napus</i> L.) leaves under drought stress. Drought treatment for 10 days provoked oxidative stress, as evidenced by the increase in O<sub>2</sub><sup>•−</sup> and H<sub>2</sub>O<sub>2</sub> concentrations, and lipid peroxidation levels, and a decrease in leaf water potential. Drought-induced oxidative responses were significantly alleviated by DMTU treatment. The accumulation of O<sub>2</sub><sup>•−</sup> and H<sub>2</sub>O<sub>2</sub> in drought-treated plants coincided with the enhanced expression of the <i>NADPH oxidase</i> and <i>Cu/Zn-SOD</i> genes, leading to an up-regulation in oxidative signal-inducible 1 (<i>OXI1</i>) and mitogen-activated protein kinase 6 (<i>MAPK6</i>), with a concomitant increase in ABA levels and the up-regulation of ABA-related genes. DMTU treatment under drought largely suppressed the drought-responsive up-regulation of these genes by depressing ABA responses through an antagonistic interaction with salicylic acid (SA). DMTU treatment also alleviated the drought-induced loss of reducing potential in GSH- and NADPH-based redox by the enhanced expression of glutathione reductase 1 (<i>GR1</i>) and up-regulation of oxidoreductase genes (<i>TRXh5</i> and <i>GRXC9</i>). These results indicate that DMTU effectively alleviates drought-induced oxidative responses by suppressing ABA-mediated oxidative burst signaling in an antagonistic regulation of SA.https://www.mdpi.com/2076-3921/11/11/2283abscisic aciddimethylthioureadrought stressredox signalingsalicylic acid |
| spellingShingle | Bok-Rye Lee Van Hien La Sang-Hyun Park Md Al Mamun Dong-Won Bae Tae-Hwan Kim Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves Antioxidants abscisic acid dimethylthiourea drought stress redox signaling salicylic acid |
| title | Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves |
| title_full | Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves |
| title_fullStr | Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves |
| title_full_unstemmed | Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves |
| title_short | Dimethylthiourea Alleviates Drought Stress by Suppressing Hydrogen Peroxide-Dependent Abscisic Acid-Mediated Oxidative Responses in an Antagonistic Interaction with Salicylic Acid in <i>Brassica napus</i> Leaves |
| title_sort | dimethylthiourea alleviates drought stress by suppressing hydrogen peroxide dependent abscisic acid mediated oxidative responses in an antagonistic interaction with salicylic acid in i brassica napus i leaves |
| topic | abscisic acid dimethylthiourea drought stress redox signaling salicylic acid |
| url | https://www.mdpi.com/2076-3921/11/11/2283 |
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