CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS
Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and stren...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2015-04-01
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| Series: | Ciência Animal Brasileira |
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| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/24240/18497 |
| _version_ | 1818267135240044544 |
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| author | Júlio Cesar Oliveira Dias Madriano Christilis da Rocha Santos Jurandy Mauro Penitente Filho Gisele Dias Oliveira Vivian Rachel Araujo Mendes Antonio Bento Mancio |
| author_facet | Júlio Cesar Oliveira Dias Madriano Christilis da Rocha Santos Jurandy Mauro Penitente Filho Gisele Dias Oliveira Vivian Rachel Araujo Mendes Antonio Bento Mancio |
| author_sort | Júlio Cesar Oliveira Dias |
| collection | DOAJ |
| description | Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology. |
| first_indexed | 2024-12-12T20:17:47Z |
| format | Article |
| id | doaj.art-266574703371417d97de6d3c00d8c422 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-12-12T20:17:47Z |
| publishDate | 2015-04-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-266574703371417d97de6d3c00d8c4222022-12-22T00:13:19ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912015-04-0116224325010.1590/1089-6891v16i224240CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRISJúlio Cesar Oliveira Dias0Madriano Christilis da Rocha Santos1Jurandy Mauro Penitente Filho2Gisele Dias Oliveira3Vivian Rachel Araujo Mendes4Antonio Bento Mancio5 Médico Veterinário, Doutor, Viçosa, MG, Brasil - diasjuliovet@yahoo.com.brMédicos Veterinários, Doutores, Viçosa, MG, Brasil.Médicos Veterinários, Doutores, Viçosa, MG, Brasil.Médicas Veterinárias, Mestres, Viçosa, MG, Brasil.Médicas Veterinárias, Mestres, Viçosa, MG, Brasil.Professor Doutor Universidade Federal de Viçosa, Viçosa, MG, Brasil.Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.http://www.revistas.ufg.br/index.php/vet/article/view/24240/18497extendergoatsemenspermatozoa |
| spellingShingle | Júlio Cesar Oliveira Dias Madriano Christilis da Rocha Santos Jurandy Mauro Penitente Filho Gisele Dias Oliveira Vivian Rachel Araujo Mendes Antonio Bento Mancio CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS Ciência Animal Brasileira extender goat semen spermatozoa |
| title | CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
| title_full | CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
| title_fullStr | CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
| title_full_unstemmed | CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
| title_short | CARACTERÍSTICAS DO SÊMEN CAPRINO DESCONGELADO APÓS A ADIÇÃO DE RINGER LACTATO, CITRATO DE SÓDIO E SOLUÇÃO TRIS |
| title_sort | caracteristicas do semen caprino descongelado apos a adicao de ringer lactato citrato de sodio e solucao tris |
| topic | extender goat semen spermatozoa |
| url | http://www.revistas.ufg.br/index.php/vet/article/view/24240/18497 |
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