Salvianolic acid B alleviates LPS-induced acute lung injury in mice
Objective To study whether salvianolic acid B(SAB) can reduce lipopolysaccharide(LPS)-induced acute lung injury. Methods Fourty-eight mice were randomly divided into control group, model group (intratracheal instillation of LPS), SAB low/medium/high dose intervention group, and sulforaphane positi...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200662.pdf |
| _version_ | 1827390572730515456 |
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| author | LI Yu-ting, LUO Le, YIN Su-juan, ZHU Xiao-lan |
| author_facet | LI Yu-ting, LUO Le, YIN Su-juan, ZHU Xiao-lan |
| author_sort | LI Yu-ting, LUO Le, YIN Su-juan, ZHU Xiao-lan |
| collection | DOAJ |
| description | Objective To study whether salvianolic acid B(SAB) can reduce lipopolysaccharide(LPS)-induced acute lung injury. Methods Fourty-eight mice were randomly divided into control group, model group (intratracheal instillation of LPS), SAB low/medium/high dose intervention group, and sulforaphane positive control group. The lung wet / dry weight ratio (W/D) was recorded; The protein concentration of alveolar lavage fluid (BALF) was detected; Lung tissue pathological damage was evaluated by HE staining and microoscopy; TNF-α,IL-1β and IL-6 in BALF were detected by ELISA.Western blot was used to detect the protein expression of Nrf2, NQO1, HO1, Keap1,NF-κB and p-NF-κB in lung tissue. Results Compared with the control group, pathological changes were observed in the lung tissue of the model group, W/D, protein concentration in BALF and inflammatory cytokine content were significantly increased (P＜0.05). Compared with the model group, SAB intervention group had significant changes (P＜0.05) that alleviated the oxidative stress induced by LPS, increased the expression of Keap1(P＜0.01), increased the expression of Nrf2, NQO1 and HO1 (P＜0.01), and inhibited the activation of NF-κB and p-NF-κB induced by LPS. Conclusions SAB can alleviate LPS-induced acute lung injury of mice. |
| first_indexed | 2024-03-08T16:54:40Z |
| format | Article |
| id | doaj.art-26675cbbbae142a1878a7bdc96a74cb0 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:40Z |
| publishDate | 2021-03-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-26675cbbbae142a1878a7bdc96a74cb02024-01-05T03:04:40ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-03-01413376381Salvianolic acid B alleviates LPS-induced acute lung injury in miceLI Yu-ting, LUO Le, YIN Su-juan, ZHU Xiao-lan01. College of Medicine,Yongzhou Vocational Technical College, Yongzhou 425006; ;2. School of Pharmaceutical Sciences,Central South University,Changsha 410205,ChinaObjective To study whether salvianolic acid B(SAB) can reduce lipopolysaccharide(LPS)-induced acute lung injury. Methods Fourty-eight mice were randomly divided into control group, model group (intratracheal instillation of LPS), SAB low/medium/high dose intervention group, and sulforaphane positive control group. The lung wet / dry weight ratio (W/D) was recorded; The protein concentration of alveolar lavage fluid (BALF) was detected; Lung tissue pathological damage was evaluated by HE staining and microoscopy; TNF-α,IL-1β and IL-6 in BALF were detected by ELISA.Western blot was used to detect the protein expression of Nrf2, NQO1, HO1, Keap1,NF-κB and p-NF-κB in lung tissue. Results Compared with the control group, pathological changes were observed in the lung tissue of the model group, W/D, protein concentration in BALF and inflammatory cytokine content were significantly increased (P＜0.05). Compared with the model group, SAB intervention group had significant changes (P＜0.05) that alleviated the oxidative stress induced by LPS, increased the expression of Keap1(P＜0.01), increased the expression of Nrf2, NQO1 and HO1 (P＜0.01), and inhibited the activation of NF-κB and p-NF-κB induced by LPS. Conclusions SAB can alleviate LPS-induced acute lung injury of mice.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200662.pdfsalvianolic acid b|acute lung injury|oxidative stress |
| spellingShingle | LI Yu-ting, LUO Le, YIN Su-juan, ZHU Xiao-lan Salvianolic acid B alleviates LPS-induced acute lung injury in mice Jichu yixue yu linchuang salvianolic acid b|acute lung injury|oxidative stress |
| title | Salvianolic acid B alleviates LPS-induced acute lung injury in mice |
| title_full | Salvianolic acid B alleviates LPS-induced acute lung injury in mice |
| title_fullStr | Salvianolic acid B alleviates LPS-induced acute lung injury in mice |
| title_full_unstemmed | Salvianolic acid B alleviates LPS-induced acute lung injury in mice |
| title_short | Salvianolic acid B alleviates LPS-induced acute lung injury in mice |
| title_sort | salvianolic acid b alleviates lps induced acute lung injury in mice |
| topic | salvianolic acid b|acute lung injury|oxidative stress |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200662.pdf |
| work_keys_str_mv | AT liyutingluoleyinsujuanzhuxiaolan salvianolicacidballeviateslpsinducedacutelunginjuryinmice |