Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia
The study was aimed to investigate antimicrobial and antioxidant activity of ethanol extracts obtain from leaves, seeds and sheaths of R. pseudoacacia. Total phenolic content (TPC, Folin-Ciocalteu method), antioxidant activity (TEAC assay) and antimicrobial activity (agar disk diffusion met...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2014-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400049M.pdf |
| _version_ | 1818751738139639808 |
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| author | Marinas Ioana C. Oprea Eliza Geana Elisabeta-Irina Chifiriuc Carmen Lazar Veronica |
| author_facet | Marinas Ioana C. Oprea Eliza Geana Elisabeta-Irina Chifiriuc Carmen Lazar Veronica |
| author_sort | Marinas Ioana C. |
| collection | DOAJ |
| description | The study was aimed to investigate antimicrobial and antioxidant activity of
ethanol extracts obtain from leaves, seeds and sheaths of R. pseudoacacia.
Total phenolic content (TPC, Folin-Ciocalteu method), antioxidant activity
(TEAC assay) and antimicrobial activity (agar disk diffusion method and broth
dilution method) of vegetative and reproductive organs of R. pseudoacacia
were done. The highest content of polyphenols (expressed as gallic acid
equivalents, GAE) obtained for R. pseudoacacia leaves extract (266.7 μg GAE
mL-1 extract) followed by seeds extract (232.2 μg GAE mL-1 extract). The HPLC
analysis showed presence of catechin (0.925 μg mL-1), rutin (0.831 μg mL-1),
resveratrol (0.664 μg mL-1) and quercetin (0.456 μg mL-1) in leaves and
catechin (0.127 μg mL-1), epicatechin (0.239 μg mL-1) and rutin (0.231 μg
mL-1) in seeds extract. The results showed that the studied extracts
exhibited a selective antimicrobial effect directed against Gram-positive
(Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Pseudomonas
aeruginosa, Klebsiella pneumonie and Escherichia coli) bacterial strains. The
combination leaves extract / antibiotic had the highest synergistic effect
when compared to seeds and sheaths extracts. The same extract with
penicillin, kanamycin and rifampin had highest synergetic effect against
methicillin resistant S. aureus strain (MRSA), a strain which it has gained a
great interest of microbiologists within past decades. The chemical
characterization of ethanol extracts from the vegetative and reproductive
organs of Robinia pseudoacacia, synergistic effects of certain antibiotics
and acacia extracts, potential to increase antimicrobial activity of some
commercial antibiotics against MRSA were done for the first time. |
| first_indexed | 2024-12-18T04:40:20Z |
| format | Article |
| id | doaj.art-266abd86c3ab44c9b48015274f431552 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-18T04:40:20Z |
| publishDate | 2014-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-266abd86c3ab44c9b48015274f4315522022-12-21T21:20:44ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212014-01-0179111363137810.2298/JSC140304049M0352-51391400049MAntimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacaciaMarinas Ioana C.0Oprea Eliza1Geana Elisabeta-Irina2Chifiriuc Carmen3Lazar Veronica4University of Bucharest, Faculty of Biology, Microbiology Department, Bucharest, RomaniaUniversity of Bucharest, Faculty of Chemistry, Bucharest, RomaniaNational R&D Institute for Cryogenics and Isotopic Technologies, ICIT Rm. Valcea, PO Raureni, Rm. Valcea, RomaniaUniversity of Bucharest, Faculty of Biology, Microbiology Department, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Microbiology Department, Bucharest, RomaniaThe study was aimed to investigate antimicrobial and antioxidant activity of ethanol extracts obtain from leaves, seeds and sheaths of R. pseudoacacia. Total phenolic content (TPC, Folin-Ciocalteu method), antioxidant activity (TEAC assay) and antimicrobial activity (agar disk diffusion method and broth dilution method) of vegetative and reproductive organs of R. pseudoacacia were done. The highest content of polyphenols (expressed as gallic acid equivalents, GAE) obtained for R. pseudoacacia leaves extract (266.7 μg GAE mL-1 extract) followed by seeds extract (232.2 μg GAE mL-1 extract). The HPLC analysis showed presence of catechin (0.925 μg mL-1), rutin (0.831 μg mL-1), resveratrol (0.664 μg mL-1) and quercetin (0.456 μg mL-1) in leaves and catechin (0.127 μg mL-1), epicatechin (0.239 μg mL-1) and rutin (0.231 μg mL-1) in seeds extract. The results showed that the studied extracts exhibited a selective antimicrobial effect directed against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Pseudomonas aeruginosa, Klebsiella pneumonie and Escherichia coli) bacterial strains. The combination leaves extract / antibiotic had the highest synergistic effect when compared to seeds and sheaths extracts. The same extract with penicillin, kanamycin and rifampin had highest synergetic effect against methicillin resistant S. aureus strain (MRSA), a strain which it has gained a great interest of microbiologists within past decades. The chemical characterization of ethanol extracts from the vegetative and reproductive organs of Robinia pseudoacacia, synergistic effects of certain antibiotics and acacia extracts, potential to increase antimicrobial activity of some commercial antibiotics against MRSA were done for the first time.http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400049M.pdfantimicrobial activityantioxidant activityRobinia pseudoacaciaantibiotics |
| spellingShingle | Marinas Ioana C. Oprea Eliza Geana Elisabeta-Irina Chifiriuc Carmen Lazar Veronica Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia Journal of the Serbian Chemical Society antimicrobial activity antioxidant activity Robinia pseudoacacia antibiotics |
| title | Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia |
| title_full | Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia |
| title_fullStr | Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia |
| title_full_unstemmed | Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia |
| title_short | Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia |
| title_sort | antimicrobial and antioxidant activity of the vegetative and reproductive organs of robinia pseudoacacia |
| topic | antimicrobial activity antioxidant activity Robinia pseudoacacia antibiotics |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391400049M.pdf |
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