Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications
Bromelain (Bro) is a multiprotein complex extracted from the pineapple plant Ananas comosus, composed of at least eight cysteine proteases. Bro has a wide range of applications in medicine and industry, where the stability of its active proteases is always a major concern. The present study describe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/8/1143 |
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| author | Adrianne M. M. Brito Vitor Oliveira Marcelo Y. Icimoto Iseli L. Nantes-Cardoso |
| author_facet | Adrianne M. M. Brito Vitor Oliveira Marcelo Y. Icimoto Iseli L. Nantes-Cardoso |
| author_sort | Adrianne M. M. Brito |
| collection | DOAJ |
| description | Bromelain (Bro) is a multiprotein complex extracted from the pineapple plant Ananas comosus, composed of at least eight cysteine proteases. Bro has a wide range of applications in medicine and industry, where the stability of its active proteases is always a major concern. The present study describes the improvement of stability and gain of specific activity in the enzymatic content of Bro immobilized on gold nanoparticles (GNPs). GNPs were synthesized in situ using Bro as the reducing and stabilizing agents and characterized by surface plasmon resonance and transmission electron microscopy. Consistent with the structural changes observed by circular dichroism analysis, the association with GNPs affected enzyme activity. The active Bro immobilized on GNPs (NanoBro) remained stable under storage and gained thermal stability consistent with a thermophilic enzyme. Proteolytic assays were performed on type I collagen membranes using fluorescence spectroscopy of O-phthaldialdehyde (OPA), changes in the membrane superficial structure, and topography by scanning electron microscopy, FTIR, and scanning laser confocal microscopy. Another characteristic of the NanoBro observed was the significant increase in susceptibility to the inhibitory effect of E-64, indicating a gain in cysteine protease activity. The higher stability and specific activity of NanoBro contributed to the broadening and improvement of Bro applications. |
| first_indexed | 2024-03-10T08:29:00Z |
| format | Article |
| id | doaj.art-266c96e95d1447a3872aae3b024d469b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:29:00Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-266c96e95d1447a3872aae3b024d469b2023-11-22T09:13:07ZengMDPI AGPharmaceutics1999-49232021-07-01138114310.3390/pharmaceutics13081143Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic ApplicationsAdrianne M. M. Brito0Vitor Oliveira1Marcelo Y. Icimoto2Iseli L. Nantes-Cardoso3Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André CEP 09210-580, SP, BrazilDepartamento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo CEP 04039-032, SP, BrazilDepartamento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo CEP 04039-032, SP, BrazilCentro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André CEP 09210-580, SP, BrazilBromelain (Bro) is a multiprotein complex extracted from the pineapple plant Ananas comosus, composed of at least eight cysteine proteases. Bro has a wide range of applications in medicine and industry, where the stability of its active proteases is always a major concern. The present study describes the improvement of stability and gain of specific activity in the enzymatic content of Bro immobilized on gold nanoparticles (GNPs). GNPs were synthesized in situ using Bro as the reducing and stabilizing agents and characterized by surface plasmon resonance and transmission electron microscopy. Consistent with the structural changes observed by circular dichroism analysis, the association with GNPs affected enzyme activity. The active Bro immobilized on GNPs (NanoBro) remained stable under storage and gained thermal stability consistent with a thermophilic enzyme. Proteolytic assays were performed on type I collagen membranes using fluorescence spectroscopy of O-phthaldialdehyde (OPA), changes in the membrane superficial structure, and topography by scanning electron microscopy, FTIR, and scanning laser confocal microscopy. Another characteristic of the NanoBro observed was the significant increase in susceptibility to the inhibitory effect of E-64, indicating a gain in cysteine protease activity. The higher stability and specific activity of NanoBro contributed to the broadening and improvement of Bro applications.https://www.mdpi.com/1999-4923/13/8/1143bromelaingold nanoparticlescollagennanotechnologycysteine protease |
| spellingShingle | Adrianne M. M. Brito Vitor Oliveira Marcelo Y. Icimoto Iseli L. Nantes-Cardoso Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications Pharmaceutics bromelain gold nanoparticles collagen nanotechnology cysteine protease |
| title | Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications |
| title_full | Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications |
| title_fullStr | Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications |
| title_full_unstemmed | Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications |
| title_short | Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications |
| title_sort | collagenase activity of bromelain immobilized at gold nanoparticle interfaces for therapeutic applications |
| topic | bromelain gold nanoparticles collagen nanotechnology cysteine protease |
| url | https://www.mdpi.com/1999-4923/13/8/1143 |
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