The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests

The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests

An assay was developed to detect the potato spindle tuber viroid (PSTVd), a dangerous plant pathogen that causes crop damage resulting in economic losses in the potato agriculture sector. The assay was based on the reverse transcription and recombinase polymerase amplification (RT-RPA) of PSTVd RNA...

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Bibliographic Details
Main Authors: Aleksandr V. Ivanov, Irina V. Shmyglya, Anatoly V. Zherdev, Boris B. Dzantiev, Irina V. Safenkova
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Plants
Subjects:
isothermal amplification
lateral flow assay
potato spindle tuber viroid
recombinase polymerase amplification
Online Access:https://www.mdpi.com/2223-7747/9/10/1369

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