Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023
In 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusa...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2024-03-01
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| Series: | Research in Plant Disease |
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| Online Access: | http://online-rpd.org/upload/pdf/RPD-2024-30-1-26.pdf |
| _version_ | 1797208840418099200 |
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| author | Miah Bae Namsuk Kim Sang Woo Kim Sangyeon Ju Byungyeon Kim Soo Man Hwang MeeKyoung Kim Mi-Ri Park |
| author_facet | Miah Bae Namsuk Kim Sang Woo Kim Sangyeon Ju Byungyeon Kim Soo Man Hwang MeeKyoung Kim Mi-Ri Park |
| author_sort | Miah Bae |
| collection | DOAJ |
| description | In 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusarium oxysporum by microscope. To confirm the presence of F. oxysporum in the samples, polymerase chain reaction was performed using primers specific for F. oxysporum; the resulting sequence showed 99.11% identity with F. oxysporum. To confirm the pathogenicity of the F. oxysporum (CW) isolated from the samples, healthy paprika plants were inoculated with F. oxysporum CW and damping-off symptoms were observed 2 weeks later. To investigate whether the damping-off disease outbreak in Cheorwon-gun was caused by F. oxysporum-contaminated seeds, 100 paprika seeds were disinfected and placed in Murashige and Skoog medium. Typical pink F. oxysporum hyphae were found only in control non-disinfected seeds. An 18S rRNA-based and a TEF gene-based phylogenetic analysis showed that the F. oxysporum CW isolate was not grouped with a F. oxysporum isolate reported from Cheorwon-gun in 2019. This study is the first report that an outbreak of damping-off disease in paprika in Cheorwon-gun, Gangwon-do, Korea, was caused by contamination of F. oxysporum seeds. |
| first_indexed | 2024-04-24T09:45:12Z |
| format | Article |
| id | doaj.art-2674b3e7756a4e78ae4030062f4f7c9e |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-04-24T09:45:12Z |
| publishDate | 2024-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-2674b3e7756a4e78ae4030062f4f7c9e2024-04-15T02:29:03ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912024-03-01301263310.5423/RPD.2024.30.1.261828Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023Miah Bae0Namsuk Kim1Sang Woo Kim2Sangyeon Ju3Byungyeon Kim4Soo Man Hwang5MeeKyoung Kim6Mi-Ri Park7Division of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute, Cheorwon 24047, KoreaDivision of Agricultural Policy, Cheorwon Agricultural Technology Center, Cheorwon 24023, KoreaDivision of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute, Cheorwon 24047, KoreaDivision of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute, Cheorwon 24047, KoreaDivision of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute, Cheorwon 24047, KoreaDivision of Agricultural Policy, Cheorwon Agricultural Technology Center, Cheorwon 24023, KoreaDivision of Agricultural Policy, Cheorwon Agricultural Technology Center, Cheorwon 24023, KoreaDivision of Region-Specific Industries Fostering, Cheorwon Plasma Research Institute, Cheorwon 24047, KoreaIn 2023, symptoms like damping-off disease were observed in 74 paprika growing in greenhouses in Cheorwon-gun, Gangwon-do, Korea. In this study, we tried to find the cause of the damping-off disease outbreak. We collected symptomatic seedlings and observed the typical crescent-shaped conidia of Fusarium oxysporum by microscope. To confirm the presence of F. oxysporum in the samples, polymerase chain reaction was performed using primers specific for F. oxysporum; the resulting sequence showed 99.11% identity with F. oxysporum. To confirm the pathogenicity of the F. oxysporum (CW) isolated from the samples, healthy paprika plants were inoculated with F. oxysporum CW and damping-off symptoms were observed 2 weeks later. To investigate whether the damping-off disease outbreak in Cheorwon-gun was caused by F. oxysporum-contaminated seeds, 100 paprika seeds were disinfected and placed in Murashige and Skoog medium. Typical pink F. oxysporum hyphae were found only in control non-disinfected seeds. An 18S rRNA-based and a TEF gene-based phylogenetic analysis showed that the F. oxysporum CW isolate was not grouped with a F. oxysporum isolate reported from Cheorwon-gun in 2019. This study is the first report that an outbreak of damping-off disease in paprika in Cheorwon-gun, Gangwon-do, Korea, was caused by contamination of F. oxysporum seeds.http://online-rpd.org/upload/pdf/RPD-2024-30-1-26.pdfdamping-off diseasefusarium oxysporumoutbreakpaprikaseed contamination |
| spellingShingle | Miah Bae Namsuk Kim Sang Woo Kim Sangyeon Ju Byungyeon Kim Soo Man Hwang MeeKyoung Kim Mi-Ri Park Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 Research in Plant Disease damping-off disease fusarium oxysporum outbreak paprika seed contamination |
| title | Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 |
| title_full | Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 |
| title_fullStr | Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 |
| title_full_unstemmed | Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 |
| title_short | Paprika Damping-off Outbreak Caused by Fusarium oxysporum Contaminated Seeds in Cheorwon Province in 2023 |
| title_sort | paprika damping off outbreak caused by fusarium oxysporum contaminated seeds in cheorwon province in 2023 |
| topic | damping-off disease fusarium oxysporum outbreak paprika seed contamination |
| url | http://online-rpd.org/upload/pdf/RPD-2024-30-1-26.pdf |
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