A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assiste...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07022-0 |
| _version_ | 1818751474579013632 |
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| author | Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana |
| author_facet | Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana |
| author_sort | Elena López-Girona |
| collection | DOAJ |
| description | Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE.6G281100 as a candidate gene for flat shape in peach. |
| first_indexed | 2024-12-18T04:36:09Z |
| format | Article |
| id | doaj.art-26758a7bebb84311bc59c39193b5e910 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:36:09Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-26758a7bebb84311bc59c39193b5e9102022-12-21T21:20:51ZengNature PortfolioScientific Reports2045-23222017-07-017111110.1038/s41598-017-07022-0A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peachElena López-Girona0Yu Zhang1Iban Eduardo2José Ramón Hernández Mora3Konstantinos G. Alexiou4Pere Arús5María José Aranzana6IRTA (Institut de Recerca i Tecnologia Agroalimentàries)Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, BellaterraIRTA (Institut de Recerca i Tecnologia Agroalimentàries)IRTA (Institut de Recerca i Tecnologia Agroalimentàries)IRTA (Institut de Recerca i Tecnologia Agroalimentàries)IRTA (Institut de Recerca i Tecnologia Agroalimentàries)IRTA (Institut de Recerca i Tecnologia Agroalimentàries)Abstract In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE.6G281100 as a candidate gene for flat shape in peach.https://doi.org/10.1038/s41598-017-07022-0 |
| spellingShingle | Elena López-Girona Yu Zhang Iban Eduardo José Ramón Hernández Mora Konstantinos G. Alexiou Pere Arús María José Aranzana A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach Scientific Reports |
| title | A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach |
| title_full | A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach |
| title_fullStr | A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach |
| title_full_unstemmed | A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach |
| title_short | A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach |
| title_sort | deletion affecting an lrr rlk gene co segregates with the fruit flat shape trait in peach |
| url | https://doi.org/10.1038/s41598-017-07022-0 |
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