Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice
Introduction: Isoniazid-induced seizure, often described as Status Epilepticus (SE), is an emergency condition characterized by repeated convulsive episodes that responds poorly to the currently available anticonvulsant drugs. The current study aimed at ascertaining the effect of Jobelyn® (JB), an A...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2018-11-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-334-5&slc_lang=en&sid=1 |
| _version_ | 1797283082307371008 |
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| author | Stephen Asehinde Abayomi Ajayi Adewale Bakre Osarume Omorogbe Adaeze Adebesin Solomon Umukoro |
| author_facet | Stephen Asehinde Abayomi Ajayi Adewale Bakre Osarume Omorogbe Adaeze Adebesin Solomon Umukoro |
| author_sort | Stephen Asehinde |
| collection | DOAJ |
| description | Introduction: Isoniazid-induced seizure, often described as Status Epilepticus (SE), is an emergency condition characterized by repeated convulsive episodes that responds poorly to the currently available anticonvulsant drugs. The current study aimed at ascertaining the effect of Jobelyn® (JB), an African dietary supplement, on seizures, altered oxidative stress, and glutamate decarboxylase activity induced by isoniazid in mice.
Methods: A total of 6 mice received JB (10-50 mg/kg, PO), pyridoxine (300 mg/kg), diazepam (5 mg/kg), or distilled water (10 mL/kg) 30 minutes prior to the induction of SE with injection of isoniazid (300 mg/kg, IP). Thereafter, the mice were observed for the onset of convulsions for a period of two hours. Moreover, the effect of JB on Glutamate Decarboxylase (GAD) activity and biomarkers of oxidative stress (glutathione and malondialdehyde) was also evaluated in the brain homogenates of another set of isoniazid-treated mice.
Results: JB (50 mg/kg, PO) prolonged the latency to convulsions, but could not prevent the occurrence of seizure episodes caused by isoniazid. Moreover, JB neither showed any protection against death nor delayed the latency to death caused by isoniazid. However, this dose of JB positively modulated the concentrations of malondialdehyde and glutathione in the brains of mice treated with isoniazid. The activity of GAD, the enzyme responsible for GABA synthesis, increased by JB, which suggested enhanced GABAergic neurotransmission.
Conclusion: The current study findings suggest that JB prolongs the latency to convulsions, enhances GABAergic neurotransmission, and demonstrates anti-oxidative effect in isoniazid-treated mice. |
| first_indexed | 2024-03-07T17:24:59Z |
| format | Article |
| id | doaj.art-2676114df766476294020c020dcac9c2 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:24:59Z |
| publishDate | 2018-11-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-2676114df766476294020c020dcac9c22024-03-02T19:16:35ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422018-11-0196389396Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in MiceStephen Asehinde0Abayomi Ajayi1Adewale Bakre2Osarume Omorogbe3Adaeze Adebesin4Solomon Umukoro5 Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. Introduction: Isoniazid-induced seizure, often described as Status Epilepticus (SE), is an emergency condition characterized by repeated convulsive episodes that responds poorly to the currently available anticonvulsant drugs. The current study aimed at ascertaining the effect of Jobelyn® (JB), an African dietary supplement, on seizures, altered oxidative stress, and glutamate decarboxylase activity induced by isoniazid in mice. Methods: A total of 6 mice received JB (10-50 mg/kg, PO), pyridoxine (300 mg/kg), diazepam (5 mg/kg), or distilled water (10 mL/kg) 30 minutes prior to the induction of SE with injection of isoniazid (300 mg/kg, IP). Thereafter, the mice were observed for the onset of convulsions for a period of two hours. Moreover, the effect of JB on Glutamate Decarboxylase (GAD) activity and biomarkers of oxidative stress (glutathione and malondialdehyde) was also evaluated in the brain homogenates of another set of isoniazid-treated mice. Results: JB (50 mg/kg, PO) prolonged the latency to convulsions, but could not prevent the occurrence of seizure episodes caused by isoniazid. Moreover, JB neither showed any protection against death nor delayed the latency to death caused by isoniazid. However, this dose of JB positively modulated the concentrations of malondialdehyde and glutathione in the brains of mice treated with isoniazid. The activity of GAD, the enzyme responsible for GABA synthesis, increased by JB, which suggested enhanced GABAergic neurotransmission. Conclusion: The current study findings suggest that JB prolongs the latency to convulsions, enhances GABAergic neurotransmission, and demonstrates anti-oxidative effect in isoniazid-treated mice.http://bcn.iums.ac.ir/browse.php?a_code=A-10-334-5&slc_lang=en&sid=1Sorghum bicolorSeizuresIsoniazidGlutamate decarboxylaseGABA |
| spellingShingle | Stephen Asehinde Abayomi Ajayi Adewale Bakre Osarume Omorogbe Adaeze Adebesin Solomon Umukoro Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice Basic and Clinical Neuroscience Sorghum bicolor Seizures Isoniazid Glutamate decarboxylase GABA |
| title | Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice |
| title_full | Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice |
| title_fullStr | Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice |
| title_full_unstemmed | Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice |
| title_short | Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice |
| title_sort | effects of jobelyn r on isoniazid induced seizures biomarkers of oxidative stress and glutamate decarboxylase activity in mice |
| topic | Sorghum bicolor Seizures Isoniazid Glutamate decarboxylase GABA |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-334-5&slc_lang=en&sid=1 |
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