Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2012-08-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31767 |
| _version_ | 1818541484550389760 |
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| author | César Augusto Vargas García Henry Arguello Fuentes Rodrigo Gonzalo Torres Sáez |
| author_facet | César Augusto Vargas García Henry Arguello Fuentes Rodrigo Gonzalo Torres Sáez |
| author_sort | César Augusto Vargas García |
| collection | DOAJ |
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mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} --> <!--[endif] --> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Título en ingles: Prediction of genome scale<span style="mso-spacerun: yes;"> </span>of <em style="mso-bidi-font-style: normal;">Saccharomyces cerevisiae</em> by flux balance analysis</span></strong></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-no-proof: yes;">Resumen: </span></strong><span style="font-size: 10.0pt; mso-bidi-font-weight: bold; mso-no-proof: yes;">El microorganismo <em style="mso-bidi-font-style: normal;">Saccharomyces cerevisiae</em> cuenta con gran número de modelos biológicos conocidos como reconstrucciones, las cuales pueden ser a escala genómica. De estas reconstrucciones a escala genómica provienen los modelos matemáticos, también llamados modelos estequiométricos. Una de las técnicas más usadas para estudiar estos modelos es el Análisis de Balance de Flujos (FBA). El proposito del FBA es predecir el crecimiento del microorganismo bajo estudio, y la producción y consumo de componentes como el etanol, CO<sub>2</sub> glicerol, sucinato, acetato y piruvato. Para determinar si las predicciones obtenidas mediante FBA son únicas se utiliza la técnica de Análisis de Variabilidad Flujos (FVA). El presente trabajo muestra los resultados de aplicar el FBA a la reconstrucción reciente del microorganismo <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>, la denominada iMM904 y los compara con un conjunto de datos experimentales presente en la literatura. Este trabajo también estudia la existencia de múltiples predicciones FBA utilizando la técnica FVA. Los resultados ilustran que es posible predecir el crecimiento del microorganimo <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>, con errores entre el 11% y 28%;<span style="mso-spacerun: yes;"> </span>la producción de CO<sub>2</sub>, con errores entre el 0.3% y 4.5% y la producción de etanol, con errores entre el 11% y 13%.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-no-proof: yes;">Palabras clave</span></strong><span style="font-size: 10.0pt; mso-bidi-font-weight: bold; mso-no-proof: yes;">: analisis de balance de flujos, reconstrucción a escala genómica, iMM904, <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Abstract: </span></strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-bidi-font-weight: bold; mso-no-proof: yes;" lang="EN-US">Several biological models, named reconstructions, are used for the study of the <em style="mso-bidi-font-style: normal;">S. cerevisiae</em> microorganism. The reconstructions can be genomic scaled. Mathematical models are generated from the reconstructions and they are called stoichiometric models. The flux balance analysis (FBA) is one of the tools used for the analysis of these models. The FBA attempts to predict the evolution of the microorganism and the consumption and production of components like glucose, ethanol, glycerol, succinate, acetate and pyruvate. A Flux variability analysis (FVA) is used to determine the uniqueness of the FBA predictions. This paper shows the results of applying FBA to the iMM904 reconstruction of <em style="mso-bidi-font-style: normal;">S. cerevisiae</em> and compares them with experimental data from literature. The results in this paper show that it is possible to predict the evolution with errors between 11% and 28% ;<span style="mso-spacerun: yes;"> </span>the production of CO2 with errors between 0.3% and 4.5%; and the production of ethanol with errors between 11% and 13%, using FBA for the iMM904 model.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Keywords</span></strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-bidi-font-weight: bold; mso-no-proof: yes;" lang="EN-US">: flux balance analysis, genome scale reconstructions, iMM904, <em style="mso-bidi-font-style: normal;">S. cerevisiae</em></span></p> |
| first_indexed | 2024-12-11T22:09:53Z |
| format | Article |
| id | doaj.art-26761a4a795444c6a1108270260ed16d |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-11T22:09:53Z |
| publishDate | 2012-08-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-26761a4a795444c6a1108270260ed16d2022-12-22T00:48:49ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582012-08-011419310728018Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de FlujosCésar Augusto Vargas García0Henry Arguello Fuentes1Rodrigo Gonzalo Torres Sáez2InvestigadorInvestigadorInvestigador<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>ES-CO</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> 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mso-hansi-theme-font:minor-latin;} --> <!--[endif] --> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Título en ingles: Prediction of genome scale<span style="mso-spacerun: yes;"> </span>of <em style="mso-bidi-font-style: normal;">Saccharomyces cerevisiae</em> by flux balance analysis</span></strong></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-no-proof: yes;">Resumen: </span></strong><span style="font-size: 10.0pt; mso-bidi-font-weight: bold; mso-no-proof: yes;">El microorganismo <em style="mso-bidi-font-style: normal;">Saccharomyces cerevisiae</em> cuenta con gran número de modelos biológicos conocidos como reconstrucciones, las cuales pueden ser a escala genómica. De estas reconstrucciones a escala genómica provienen los modelos matemáticos, también llamados modelos estequiométricos. Una de las técnicas más usadas para estudiar estos modelos es el Análisis de Balance de Flujos (FBA). El proposito del FBA es predecir el crecimiento del microorganismo bajo estudio, y la producción y consumo de componentes como el etanol, CO<sub>2</sub> glicerol, sucinato, acetato y piruvato. Para determinar si las predicciones obtenidas mediante FBA son únicas se utiliza la técnica de Análisis de Variabilidad Flujos (FVA). El presente trabajo muestra los resultados de aplicar el FBA a la reconstrucción reciente del microorganismo <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>, la denominada iMM904 y los compara con un conjunto de datos experimentales presente en la literatura. Este trabajo también estudia la existencia de múltiples predicciones FBA utilizando la técnica FVA. Los resultados ilustran que es posible predecir el crecimiento del microorganimo <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>, con errores entre el 11% y 28%;<span style="mso-spacerun: yes;"> </span>la producción de CO<sub>2</sub>, con errores entre el 0.3% y 4.5% y la producción de etanol, con errores entre el 11% y 13%.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-no-proof: yes;">Palabras clave</span></strong><span style="font-size: 10.0pt; mso-bidi-font-weight: bold; mso-no-proof: yes;">: analisis de balance de flujos, reconstrucción a escala genómica, iMM904, <em style="mso-bidi-font-style: normal;">S. cerevisiae</em>.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Abstract: </span></strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-bidi-font-weight: bold; mso-no-proof: yes;" lang="EN-US">Several biological models, named reconstructions, are used for the study of the <em style="mso-bidi-font-style: normal;">S. cerevisiae</em> microorganism. The reconstructions can be genomic scaled. Mathematical models are generated from the reconstructions and they are called stoichiometric models. The flux balance analysis (FBA) is one of the tools used for the analysis of these models. The FBA attempts to predict the evolution of the microorganism and the consumption and production of components like glucose, ethanol, glycerol, succinate, acetate and pyruvate. A Flux variability analysis (FVA) is used to determine the uniqueness of the FBA predictions. This paper shows the results of applying FBA to the iMM904 reconstruction of <em style="mso-bidi-font-style: normal;">S. cerevisiae</em> and compares them with experimental data from literature. The results in this paper show that it is possible to predict the evolution with errors between 11% and 28% ;<span style="mso-spacerun: yes;"> </span>the production of CO2 with errors between 0.3% and 4.5%; and the production of ethanol with errors between 11% and 13%, using FBA for the iMM904 model.</span></p> <p class="MsoNormal" style="text-align: justify; line-height: normal; tab-stops: center 240.0pt right 475.0pt;"><strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-no-proof: yes;" lang="EN-US">Keywords</span></strong><span style="font-size: 10.0pt; mso-ansi-language: EN-US; mso-bidi-font-weight: bold; mso-no-proof: yes;" lang="EN-US">: flux balance analysis, genome scale reconstructions, iMM904, <em style="mso-bidi-font-style: normal;">S. cerevisiae</em></span></p>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31767analisis de balance de flujosreconstrucción a escala genómicaiMM904S. cerevisiaeflux balance analysisgenome scale reconstructionsiMM904S. cerevisiae |
| spellingShingle | César Augusto Vargas García Henry Arguello Fuentes Rodrigo Gonzalo Torres Sáez Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos Revista Colombiana de Biotecnología analisis de balance de flujos reconstrucción a escala genómica iMM904 S. cerevisiae flux balance analysis genome scale reconstructions iMM904 S. cerevisiae |
| title | Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos |
| title_full | Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos |
| title_fullStr | Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos |
| title_full_unstemmed | Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos |
| title_short | Predicción a escala genómica de Componentes de Saccharomyces cerevisiae mediante Análisis de Balance de Flujos |
| title_sort | prediccion a escala genomica de componentes de saccharomyces cerevisiae mediante analisis de balance de flujos |
| topic | analisis de balance de flujos reconstrucción a escala genómica iMM904 S. cerevisiae flux balance analysis genome scale reconstructions iMM904 S. cerevisiae |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31767 |
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