Progress in 7SK ribonucleoprotein structural biology
The 7SK ribonucleoprotein (RNP) is a dynamic and multifunctional regulator of RNA Polymerase II (RNAPII) transcription in metazoa. Comprised of the non-coding 7SK RNA, core proteins, and numerous accessory proteins, the most well-known 7SK RNP function is the sequestration and inactivation of the po...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1154622/full |
| _version_ | 1797859671296442368 |
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| author | Momodou B. Camara Amr M. Sobeh Catherine D. Eichhorn Catherine D. Eichhorn |
| author_facet | Momodou B. Camara Amr M. Sobeh Catherine D. Eichhorn Catherine D. Eichhorn |
| author_sort | Momodou B. Camara |
| collection | DOAJ |
| description | The 7SK ribonucleoprotein (RNP) is a dynamic and multifunctional regulator of RNA Polymerase II (RNAPII) transcription in metazoa. Comprised of the non-coding 7SK RNA, core proteins, and numerous accessory proteins, the most well-known 7SK RNP function is the sequestration and inactivation of the positive transcription elongation factor b (P-TEFb). More recently, 7SK RNP has been shown to regulate RNAPII transcription through P-TEFb-independent pathways. Due to its fundamental role in cellular function, dysregulation has been linked with human diseases including cancers, heart disease, developmental disorders, and viral infection. Significant advances in 7SK RNP structural biology have improved our understanding of 7SK RNP assembly and function. Here, we review progress in understanding the structural basis of 7SK RNA folding, biogenesis, and RNP assembly. |
| first_indexed | 2024-04-09T21:34:28Z |
| format | Article |
| id | doaj.art-267983bacd084bc8a688b176332f202b |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-09T21:34:28Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-267983bacd084bc8a688b176332f202b2023-03-27T05:03:41ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-03-011010.3389/fmolb.2023.11546221154622Progress in 7SK ribonucleoprotein structural biologyMomodou B. Camara0Amr M. Sobeh1Catherine D. Eichhorn2Catherine D. Eichhorn3Department of Chemistry, University of Nebraska, Lincoln, NE, United StatesDepartment of Chemistry, University of Nebraska, Lincoln, NE, United StatesDepartment of Chemistry, University of Nebraska, Lincoln, NE, United StatesNebraska Center for Integrated Biomolecular Communication, Lincoln, NE, United StatesThe 7SK ribonucleoprotein (RNP) is a dynamic and multifunctional regulator of RNA Polymerase II (RNAPII) transcription in metazoa. Comprised of the non-coding 7SK RNA, core proteins, and numerous accessory proteins, the most well-known 7SK RNP function is the sequestration and inactivation of the positive transcription elongation factor b (P-TEFb). More recently, 7SK RNP has been shown to regulate RNAPII transcription through P-TEFb-independent pathways. Due to its fundamental role in cellular function, dysregulation has been linked with human diseases including cancers, heart disease, developmental disorders, and viral infection. Significant advances in 7SK RNP structural biology have improved our understanding of 7SK RNP assembly and function. Here, we review progress in understanding the structural basis of 7SK RNA folding, biogenesis, and RNP assembly.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1154622/fullcryoEMsolution state NMRX-ray crystallographyRNA structural dynamicschemical probingRNA-protein interactions |
| spellingShingle | Momodou B. Camara Amr M. Sobeh Catherine D. Eichhorn Catherine D. Eichhorn Progress in 7SK ribonucleoprotein structural biology Frontiers in Molecular Biosciences cryoEM solution state NMR X-ray crystallography RNA structural dynamics chemical probing RNA-protein interactions |
| title | Progress in 7SK ribonucleoprotein structural biology |
| title_full | Progress in 7SK ribonucleoprotein structural biology |
| title_fullStr | Progress in 7SK ribonucleoprotein structural biology |
| title_full_unstemmed | Progress in 7SK ribonucleoprotein structural biology |
| title_short | Progress in 7SK ribonucleoprotein structural biology |
| title_sort | progress in 7sk ribonucleoprotein structural biology |
| topic | cryoEM solution state NMR X-ray crystallography RNA structural dynamics chemical probing RNA-protein interactions |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1154622/full |
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