Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification
Abstract Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40500-w |
| _version_ | 1797557926679805952 |
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| author | Matthew J. Powell-Palm E. Michael Henley Anthony N. Consiglio Claire Lager Brooke Chang Riley Perry Kendall Fitzgerald Jonathan Daly Boris Rubinsky Mary Hagedorn |
| author_facet | Matthew J. Powell-Palm E. Michael Henley Anthony N. Consiglio Claire Lager Brooke Chang Riley Perry Kendall Fitzgerald Jonathan Daly Boris Rubinsky Mary Hagedorn |
| author_sort | Matthew J. Powell-Palm |
| collection | DOAJ |
| description | Abstract Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materials that can only be obtained a few nights per year during spawning. Here, we report the successful cryopreservation and revival of cm-scale coral fragments via mL-scale isochoric vitrification. We demonstrate coral viability at 24 h post-thaw using a calibrated oxygen-uptake respirometry technique, and further show that the method can be applied in a passive, electronics-free configuration. Finally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for essential next steps in modulating post-thaw stress and initiating long-term growth. These findings pave the way towards an approach that can be rapidly deployed around the world to secure the biological genetic diversity of our vanishing coral reefs. |
| first_indexed | 2024-03-10T17:23:09Z |
| format | Article |
| id | doaj.art-267a201008ab4eb490c47267a37ffa7c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:23:09Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-267a201008ab4eb490c47267a37ffa7c2023-11-20T10:16:10ZengNature PortfolioNature Communications2041-17232023-08-0114111110.1038/s41467-023-40500-wCryopreservation and revival of Hawaiian stony corals using isochoric vitrificationMatthew J. Powell-Palm0E. Michael Henley1Anthony N. Consiglio2Claire Lager3Brooke Chang4Riley Perry5Kendall Fitzgerald6Jonathan Daly7Boris Rubinsky8Mary Hagedorn9J. Mike Walker ʻ66 Department of Mechanical Engineering, Texas A&M UniversitySmithsonian National Zoo and Conservation Biology InstituteDepartment of Mechanical Engineering, University of California BerkeleySmithsonian National Zoo and Conservation Biology InstituteDepartment of Mechanical Engineering, University of California BerkeleySmithsonian National Zoo and Conservation Biology InstituteSmithsonian National Zoo and Conservation Biology InstituteTaronga Institute of Science and Learning, Taronga Conservation Society AustraliaDepartment of Mechanical Engineering, University of California BerkeleySmithsonian National Zoo and Conservation Biology InstituteAbstract Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materials that can only be obtained a few nights per year during spawning. Here, we report the successful cryopreservation and revival of cm-scale coral fragments via mL-scale isochoric vitrification. We demonstrate coral viability at 24 h post-thaw using a calibrated oxygen-uptake respirometry technique, and further show that the method can be applied in a passive, electronics-free configuration. Finally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for essential next steps in modulating post-thaw stress and initiating long-term growth. These findings pave the way towards an approach that can be rapidly deployed around the world to secure the biological genetic diversity of our vanishing coral reefs.https://doi.org/10.1038/s41467-023-40500-w |
| spellingShingle | Matthew J. Powell-Palm E. Michael Henley Anthony N. Consiglio Claire Lager Brooke Chang Riley Perry Kendall Fitzgerald Jonathan Daly Boris Rubinsky Mary Hagedorn Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification Nature Communications |
| title | Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification |
| title_full | Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification |
| title_fullStr | Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification |
| title_full_unstemmed | Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification |
| title_short | Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification |
| title_sort | cryopreservation and revival of hawaiian stony corals using isochoric vitrification |
| url | https://doi.org/10.1038/s41467-023-40500-w |
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