| Summary: | The possibility of using the microorganisms <i>Pseudomonas</i> sp. 7p-81, <i>Pseudomonas putida</i> BS394(pBS216), <i>Rhodococcus erythropolis</i> s67, <i>Rhodococcus pyridinivorans</i> 5Ap, <i>Rhodococcus erythropolis</i> X5, <i>Rhodococcus pyridinivorans</i> F5 and <i>Pseudomonas veronii</i> DSM 11331<sup>T</sup> as the basis of a biosensor for the phenol index to assess water environments was studied. The adaptation of microorganisms to phenol during growth was carried out to increase the selectivity of the analytical system. The most promising microorganisms for biosensor formation were the bacteria <i>P. putida</i> BS394(pBS216). Cells were immobilized in redox-active polymers based on bovine serum albumin modified by ferrocenecarboxaldehyde and based on a composite with a carbon nanotube to increase sensitivity. The rate constants of the interaction of the redox-active polymer and the composite based on it with the biomaterial were 193.8 and 502.8 dm<sup>3</sup>/(g·s) respectively. For the biosensor created using hydrogel bovine serum albumin-ferrocene-carbon nanotubes, the lower limit of the determined phenol concentrations was 1 × 10<sup>−3</sup> mg/dm<sup>3</sup>, the sensitivity coefficient was (5.8 ± 0.2)∙10<sup>−3</sup> μA·dm<sup>3</sup>/mg, Michaelis constant K<sub>M</sub> = 230 mg/dm<sup>3</sup>, the maximum rate of the enzymatic reaction R<sub>max</sub> = 217 µA and the long-term stability of the bioanalyzer was 11 days. As a result of approbation, it was found that the urban water phenol content differed insignificantly, measured by creating a biosensor and using the standard photometric method.
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