Designing a SYBR Green Absolute Real time PCR Assay for Specific Detection and Quantification of Bacillus subtilis in Dough Used for Bread Making

In present study, a SYBR green based real time PCR assay was developed for specific detection and quantification of Bacillus subtilis in dough used for bread making. New primer pairs were designed to amplify a 212 base pair fragment of the aprE gene. Specificity of these primer pairs was confirmed w...

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Detalles Bibliográficos
Autores principales: Seyed Ali Mortazavi, Ahmad Reza Bahrami, Balal Sadeghi, Maryam Moghaddam Matin
Formato: Artículo
Lenguaje:English
Publicado: Ferdowsi University of Mashhad 2014-01-01
Colección:Journal of Cell and Molecular Research
Materias:
Acceso en línea:https://jcmr.um.ac.ir/article_27622_abd107565a13f9661191373084027998.pdf