Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age
In this study, the semen parameters, sperm chromatin integrity, antioxidant enzyme levels, and reproductive hormone levels of subfertile male subjects from Pakistan were assessed in relation to their age. Data on the demographic characteristics of the 750 study participants, including their general...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1092603/full |
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| author | Riffat Bibi Sarwat Jahan Salma Kafeel Qureshi Suhail Razak Tayyaba Afsar Ali Almajwal Mashal Kafeel Qureshi Mohammad Eid Hammadeh Houda Amor |
| author_facet | Riffat Bibi Sarwat Jahan Salma Kafeel Qureshi Suhail Razak Tayyaba Afsar Ali Almajwal Mashal Kafeel Qureshi Mohammad Eid Hammadeh Houda Amor |
| author_sort | Riffat Bibi |
| collection | DOAJ |
| description | In this study, the semen parameters, sperm chromatin integrity, antioxidant enzyme levels, and reproductive hormone levels of subfertile male subjects from Pakistan were assessed in relation to their age. Data on the demographic characteristics of the 750 study participants, including their general health, body mass index (BMI), and reproductive status, were collected from subfertile men from Pakistan. Semen and blood were collected to determine standard semen parameters, sperm chromatin dispersion (Halosperm-SCD), sperm chromatin integrity using toluidine blue (TB) staining, sperm chromatin maturity using chromomycin A3 (CMA3+) staining, and reproductive hormone (FSH, LH, prolactin and testosterone levels). The patients were divided into three groups according to their age: Group 1 included male subjects aged 30 years or less (n = 90), Group 2 included male subjects between the ages of 31 and 40 years (n = 330), and Group 3 included male subjects over 40 years of age (n = 330). Conventional semen parameters, reactive oxygen species (ROS), superoxide dismutase (SOD), guaiacol peroxidase (GPX), catalase (CAT), and lipid peroxidation (MDA) did not statistically (p > 0.05) differ with increasing male age or between different age groups. When compared to younger men (<30 years), sperm SCD (23.2 ± 0.88%) was significantly (p = 0.01) lower as compared to male patients aged >40 years (26.6 ± 0.6%). The concentration of LH, FSH, and testosterone levels were comparable between the groups (p > 0.05), while a significant (p = 0.04) increase in sperm chromatin immaturity CMA3+ (30 ± 0.71%) was observed in the old age group (>40 years) compared to the <30-year group (26.6 ± 1.03%). A positive association was observed between advanced male age and sperm chromatin dispersion (SCD) (r = 0.124, p = 0.001) and decondensation (CMA3+) (r = 0.1, p = 0.009). Despite potential limitations, this study has been carried out with extensive information on the potential risk of male age on sperm integrity. The present study demonstrated the impact of male age on male reproductive health, as these patients had a higher percentage of sperm chromatin damage (SCD) in their semen. Sperm DNA damage assessment will help in the evaluation and diagnosis of the underlying cause of poor fertility and can help clinicians in selecting the right treatment options. Male age is one of the factors that have an impact on the decline in male fertility. As a result, it is preferable for patients receiving assisted reproductive technology to be younger. |
| first_indexed | 2024-04-09T18:08:42Z |
| format | Article |
| id | doaj.art-267dbb463e15445899e61b622756339c |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-09T18:08:42Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-267dbb463e15445899e61b622756339c2023-04-14T04:49:13ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-04-011410.3389/fendo.2023.10926031092603Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male ageRiffat Bibi0Sarwat Jahan1Salma Kafeel Qureshi2Suhail Razak3Tayyaba Afsar4Ali Almajwal5Mashal Kafeel Qureshi6Mohammad Eid Hammadeh7Houda Amor8Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, PakistanDepartment of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, PakistanDepartment of Reproductive Health Sciences, Salma and Kafeel Medical Centre, Islamabad, PakistanDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Reproductive Health Sciences, Salma and Kafeel Medical Centre, Islamabad, PakistanDepartment of Obstetrics, Gynecology and Reproductive Medicine, Saarland University Clinic, Homburg, GermanyDepartment of Obstetrics, Gynecology and Reproductive Medicine, Saarland University Clinic, Homburg, GermanyIn this study, the semen parameters, sperm chromatin integrity, antioxidant enzyme levels, and reproductive hormone levels of subfertile male subjects from Pakistan were assessed in relation to their age. Data on the demographic characteristics of the 750 study participants, including their general health, body mass index (BMI), and reproductive status, were collected from subfertile men from Pakistan. Semen and blood were collected to determine standard semen parameters, sperm chromatin dispersion (Halosperm-SCD), sperm chromatin integrity using toluidine blue (TB) staining, sperm chromatin maturity using chromomycin A3 (CMA3+) staining, and reproductive hormone (FSH, LH, prolactin and testosterone levels). The patients were divided into three groups according to their age: Group 1 included male subjects aged 30 years or less (n = 90), Group 2 included male subjects between the ages of 31 and 40 years (n = 330), and Group 3 included male subjects over 40 years of age (n = 330). Conventional semen parameters, reactive oxygen species (ROS), superoxide dismutase (SOD), guaiacol peroxidase (GPX), catalase (CAT), and lipid peroxidation (MDA) did not statistically (p > 0.05) differ with increasing male age or between different age groups. When compared to younger men (<30 years), sperm SCD (23.2 ± 0.88%) was significantly (p = 0.01) lower as compared to male patients aged >40 years (26.6 ± 0.6%). The concentration of LH, FSH, and testosterone levels were comparable between the groups (p > 0.05), while a significant (p = 0.04) increase in sperm chromatin immaturity CMA3+ (30 ± 0.71%) was observed in the old age group (>40 years) compared to the <30-year group (26.6 ± 1.03%). A positive association was observed between advanced male age and sperm chromatin dispersion (SCD) (r = 0.124, p = 0.001) and decondensation (CMA3+) (r = 0.1, p = 0.009). Despite potential limitations, this study has been carried out with extensive information on the potential risk of male age on sperm integrity. The present study demonstrated the impact of male age on male reproductive health, as these patients had a higher percentage of sperm chromatin damage (SCD) in their semen. Sperm DNA damage assessment will help in the evaluation and diagnosis of the underlying cause of poor fertility and can help clinicians in selecting the right treatment options. Male age is one of the factors that have an impact on the decline in male fertility. As a result, it is preferable for patients receiving assisted reproductive technology to be younger.https://www.frontiersin.org/articles/10.3389/fendo.2023.1092603/fullsperm chromatin integrityassisted reproductive proceduressperm deoxyribose nucleic acid fragmentation indexreproductive markermale age |
| spellingShingle | Riffat Bibi Sarwat Jahan Salma Kafeel Qureshi Suhail Razak Tayyaba Afsar Ali Almajwal Mashal Kafeel Qureshi Mohammad Eid Hammadeh Houda Amor Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age Frontiers in Endocrinology sperm chromatin integrity assisted reproductive procedures sperm deoxyribose nucleic acid fragmentation index reproductive marker male age |
| title | Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| title_full | Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| title_fullStr | Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| title_full_unstemmed | Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| title_short | Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| title_sort | analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age |
| topic | sperm chromatin integrity assisted reproductive procedures sperm deoxyribose nucleic acid fragmentation index reproductive marker male age |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1092603/full |
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