Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon
Human rotavirus remains a major cause of gastroenteritis worldwide despite the availability of effective vaccines. In this study, we investigated the genetic diversity of rotaviruses circulating in Lebanon. We genetically characterized the VP4 and VP7 genes encoding the outer capsid proteins of 132...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.00317/full |
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| author | Houda H. Harastani Lina Reslan Ahmad Sabra Zainab Ali Zainab Ali Moza Hammadi Moza Hammadi Soha Ghanem Soha Ghanem Farah Hajar Farah Hajar Ghassan M. Matar Ghassan M. Matar Ghassan S. Dbaibo Ghassan S. Dbaibo Ghassan S. Dbaibo Hassan Zaraket Hassan Zaraket |
| author_facet | Houda H. Harastani Lina Reslan Ahmad Sabra Zainab Ali Zainab Ali Moza Hammadi Moza Hammadi Soha Ghanem Soha Ghanem Farah Hajar Farah Hajar Ghassan M. Matar Ghassan M. Matar Ghassan S. Dbaibo Ghassan S. Dbaibo Ghassan S. Dbaibo Hassan Zaraket Hassan Zaraket |
| author_sort | Houda H. Harastani |
| collection | DOAJ |
| description | Human rotavirus remains a major cause of gastroenteritis worldwide despite the availability of effective vaccines. In this study, we investigated the genetic diversity of rotaviruses circulating in Lebanon. We genetically characterized the VP4 and VP7 genes encoding the outer capsid proteins of 132 rotavirus-associated gastroenteritis specimens, previously identified in hospitalized children (<5 years) from 2011 to 2013 in Lebanon. These included 43 vaccine-breakthrough specimens and the remainder were from non-vaccinated subjects. Phylogenetic analysis of VP4 and VP7 genes revealed distinct clustering compared to the vaccine strains, and several substitutions were identified in the antigenic epitopes of Lebanese specimens. No unique changes were identified in the breakthrough specimens compared to non-breakthroughs that could explain the occurrence of infection in vaccinated children. Further, we report the emergence of a rare P[8] OP354-like strain with a G9 VP7 in Lebanon, possessing high genetic variability in their VP4 compared to vaccine strains. Therefore, human rotavirus strains circulating in Lebanon and globally have accumulated numerous substitutions in their antigenic sites compared to those currently used in the licensed vaccines. The successful spread and continued genetic drift of these strains over time might undermine the effectiveness of the vaccines. The effect of such changes in the antigenic sites on vaccine efficacy remains to be assessed. |
| first_indexed | 2024-12-10T14:48:55Z |
| format | Article |
| id | doaj.art-267faf6f1e8a474091346357a2df648f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T14:48:55Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-267faf6f1e8a474091346357a2df648f2022-12-22T01:44:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-02-011110.3389/fimmu.2020.00317503497Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in LebanonHouda H. Harastani0Lina Reslan1Ahmad Sabra2Zainab Ali3Zainab Ali4Moza Hammadi5Moza Hammadi6Soha Ghanem7Soha Ghanem8Farah Hajar9Farah Hajar10Ghassan M. Matar11Ghassan M. Matar12Ghassan S. Dbaibo13Ghassan S. Dbaibo14Ghassan S. Dbaibo15Hassan Zaraket16Hassan Zaraket17Faculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Experimental Pathology, Immunology, and Microbiology, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut, Beirut, LebanonDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, LebanonFaculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut, LebanonDepartment of Experimental Pathology, Immunology, and Microbiology, Faculty of Medicine, American University of Beirut, Beirut, LebanonHuman rotavirus remains a major cause of gastroenteritis worldwide despite the availability of effective vaccines. In this study, we investigated the genetic diversity of rotaviruses circulating in Lebanon. We genetically characterized the VP4 and VP7 genes encoding the outer capsid proteins of 132 rotavirus-associated gastroenteritis specimens, previously identified in hospitalized children (<5 years) from 2011 to 2013 in Lebanon. These included 43 vaccine-breakthrough specimens and the remainder were from non-vaccinated subjects. Phylogenetic analysis of VP4 and VP7 genes revealed distinct clustering compared to the vaccine strains, and several substitutions were identified in the antigenic epitopes of Lebanese specimens. No unique changes were identified in the breakthrough specimens compared to non-breakthroughs that could explain the occurrence of infection in vaccinated children. Further, we report the emergence of a rare P[8] OP354-like strain with a G9 VP7 in Lebanon, possessing high genetic variability in their VP4 compared to vaccine strains. Therefore, human rotavirus strains circulating in Lebanon and globally have accumulated numerous substitutions in their antigenic sites compared to those currently used in the licensed vaccines. The successful spread and continued genetic drift of these strains over time might undermine the effectiveness of the vaccines. The effect of such changes in the antigenic sites on vaccine efficacy remains to be assessed.https://www.frontiersin.org/article/10.3389/fimmu.2020.00317/fullhuman rotaviruscapsid proteinsdiversityvaccinebreakthroughRotarix |
| spellingShingle | Houda H. Harastani Lina Reslan Ahmad Sabra Zainab Ali Zainab Ali Moza Hammadi Moza Hammadi Soha Ghanem Soha Ghanem Farah Hajar Farah Hajar Ghassan M. Matar Ghassan M. Matar Ghassan S. Dbaibo Ghassan S. Dbaibo Ghassan S. Dbaibo Hassan Zaraket Hassan Zaraket Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon Frontiers in Immunology human rotavirus capsid proteins diversity vaccine breakthrough Rotarix |
| title | Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon |
| title_full | Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon |
| title_fullStr | Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon |
| title_full_unstemmed | Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon |
| title_short | Genetic Diversity of Human Rotavirus A Among Hospitalized Children Under-5 Years in Lebanon |
| title_sort | genetic diversity of human rotavirus a among hospitalized children under 5 years in lebanon |
| topic | human rotavirus capsid proteins diversity vaccine breakthrough Rotarix |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.00317/full |
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