Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome
Objective Innate lymphoid cells (ILCs) are a cell population implicated in the pathogenesis of various chronic inflammatory diseases, but little is known about their role in primary Sjögren’s syndrome (pSS). The aim of this study was to assess the frequency of ILC subsets in peripheral blood (PB) an...
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| Format: | Article |
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BMJ Publishing Group
2023-05-01
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| Series: | RMD Open |
| Online Access: | https://rmdopen.bmj.com/content/9/2/e003051.full |
| _version_ | 1797820014706819072 |
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| author | Renaud Felten Jacques-Eric Gottenberg Hélène Dumortier Lou Kawka Cédric Schleiss Jean-Daniel Fauny Pauline Le Van Quyen Fanny Monneaux |
| author_facet | Renaud Felten Jacques-Eric Gottenberg Hélène Dumortier Lou Kawka Cédric Schleiss Jean-Daniel Fauny Pauline Le Van Quyen Fanny Monneaux |
| author_sort | Renaud Felten |
| collection | DOAJ |
| description | Objective Innate lymphoid cells (ILCs) are a cell population implicated in the pathogenesis of various chronic inflammatory diseases, but little is known about their role in primary Sjögren’s syndrome (pSS). The aim of this study was to assess the frequency of ILC subsets in peripheral blood (PB) and their quantity and location in minor salivary glands (MSGs) in pSS.Methods The frequency of ILC subsets was analysed in the PB of patients with pSS and healthy controls (HCs) by flow cytometry. The amount and location of ILC subsets in MSGs were studied in patients with pSS and sicca controls by immunofluorescence assay.Results In PB, the frequency of ILC subsets did not differ between patients with pSS and HCs. The circulating frequency of the ILC1 subset was increased in patients with pSS with positive anti-SSA antibodies and that of the ILC3 subset was reduced in patients with pSS with glandular swelling. In MSGs, the ILC3 number was higher in lymphocytic-infiltrated than non-infiltrated tissue in patients with pSS and normal glandular tissues in sicca controls. The ILC3 subset was preferentially located at the periphery of infiltrates and was more abundant in small infiltrates of recently diagnosed pSS.Conclusion Altered ILC homeostasis mainly concerns salivary glands in pSS. Most ILCs in MSGs consist of the ILC3 subset, located at the periphery of lymphocytic infiltrates. The ILC3 subset is more abundant in smaller infiltrates and in recently diagnosed pSS. It might play a pathogenic role in the development of T and B lymphocyte infiltrates in the early stages of pSS. |
| first_indexed | 2024-03-13T09:32:06Z |
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| issn | 2056-5933 |
| language | English |
| last_indexed | 2024-03-13T09:32:06Z |
| publishDate | 2023-05-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | RMD Open |
| spelling | doaj.art-268034f72bd74e3c9d99d30e818033c12023-05-25T22:00:07ZengBMJ Publishing GroupRMD Open2056-59332023-05-019210.1136/rmdopen-2023-003051Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndromeRenaud Felten0Jacques-Eric Gottenberg1Hélène Dumortier2Lou Kawka3Cédric Schleiss4Jean-Daniel Fauny5Pauline Le Van Quyen6Fanny Monneaux72 IBMC, CNRS, UPR3572, Université de Strasbourg, Strasbourg, Alsace, France1 Rheumatology Department, National Reference Center for Rare East South-West Autoimmune Systemic Diseases RESO, Strasbourg University Hospitals, Strasbourg, Alsace, FranceLaboratoire d’Immunologie, Immunopathologie et Chimie Thérapeutique, CNRS UPR3572, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, FranceService de rhumatologie, Centre National de Référence des Maladies Autoimmunes et Systémiques Rares, Hôpitaux universitaires de Strasbourg, Strasbourg, FranceLaboratoire d’Immunologie, Immunopathologie et Chimie Thérapeutique, CNRS UPR3572, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, FranceLaboratoire d’Immunologie, Immunopathologie et Chimie Thérapeutique, CNRS UPR3572, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, FranceService d`anatomopathologie, Hôpitaux universitaires de Strasbourg, Strasbourg, FranceLaboratoire d’Immunologie, Immunopathologie et Chimie Thérapeutique, CNRS UPR3572, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, FranceObjective Innate lymphoid cells (ILCs) are a cell population implicated in the pathogenesis of various chronic inflammatory diseases, but little is known about their role in primary Sjögren’s syndrome (pSS). The aim of this study was to assess the frequency of ILC subsets in peripheral blood (PB) and their quantity and location in minor salivary glands (MSGs) in pSS.Methods The frequency of ILC subsets was analysed in the PB of patients with pSS and healthy controls (HCs) by flow cytometry. The amount and location of ILC subsets in MSGs were studied in patients with pSS and sicca controls by immunofluorescence assay.Results In PB, the frequency of ILC subsets did not differ between patients with pSS and HCs. The circulating frequency of the ILC1 subset was increased in patients with pSS with positive anti-SSA antibodies and that of the ILC3 subset was reduced in patients with pSS with glandular swelling. In MSGs, the ILC3 number was higher in lymphocytic-infiltrated than non-infiltrated tissue in patients with pSS and normal glandular tissues in sicca controls. The ILC3 subset was preferentially located at the periphery of infiltrates and was more abundant in small infiltrates of recently diagnosed pSS.Conclusion Altered ILC homeostasis mainly concerns salivary glands in pSS. Most ILCs in MSGs consist of the ILC3 subset, located at the periphery of lymphocytic infiltrates. The ILC3 subset is more abundant in smaller infiltrates and in recently diagnosed pSS. It might play a pathogenic role in the development of T and B lymphocyte infiltrates in the early stages of pSS.https://rmdopen.bmj.com/content/9/2/e003051.full |
| spellingShingle | Renaud Felten Jacques-Eric Gottenberg Hélène Dumortier Lou Kawka Cédric Schleiss Jean-Daniel Fauny Pauline Le Van Quyen Fanny Monneaux Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome RMD Open |
| title | Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome |
| title_full | Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome |
| title_fullStr | Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome |
| title_full_unstemmed | Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome |
| title_short | Alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary Sjögren’s syndrome |
| title_sort | alteration of innate lymphoid cell homeostasis mainly concerns salivary glands in primary sjogren s syndrome |
| url | https://rmdopen.bmj.com/content/9/2/e003051.full |
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