Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield
Abstract Background Rice tiller number is one of the most important factors that determine grain yield, while nitrogen is essential for the crop growth and development, especially for tiller formation. Genes involved in nitrogen use efficiency processes have been identified in the previous studies,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-02-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12284-018-0205-6 |
| _version_ | 1798043611233779712 |
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| author | Jie Wang Kai Lu Haipeng Nie Qisen Zeng Bowen Wu Junjie Qian Zhongming Fang |
| author_facet | Jie Wang Kai Lu Haipeng Nie Qisen Zeng Bowen Wu Junjie Qian Zhongming Fang |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | Abstract Background Rice tiller number is one of the most important factors that determine grain yield, while nitrogen is essential for the crop growth and development, especially for tiller formation. Genes involved in nitrogen use efficiency processes have been identified in the previous studies, however, only a small number of these genes have been found to improve grain yield by promoting tillering. Results We constructed over-expression (OX) lines and RNA-interference (Ri) lines, and selected a mutant of OsNPF7.2, a low-affinity nitrate transporter. Our analyses showed that rice tiller number and grain yield were significantly increased in OX lines, whereas Ri lines and mutant osnpf7.2 had fewer tiller number and lower grain yield. Under different nitrate concentrations, tiller buds grew faster in OX lines than in WT, but they grew slower in Ri lines and mutant osnpf7.2. These results indicated that altered expression of OsNPF7.2 plays a significant role in the control of tiller bud growth and regulation of tillering. Elevated expression of OsNPF7.2 also improved root length, root number, fresh weight, and dry weight. However, reduced expression of OsNPF7.2 had the opposite result on these characters. OsNPF7.2 OX lines showed more significantly enhanced influx of nitrate and had a higher nitrate concentration than WT. The levels of gene transcripts related to cytokinin pathway and cell cycle in tiller bud, and cytokinins concentration in tiller basal portion were higher in OX lines than that in WT, suggesting that altered expression of OsNPF7.2 controlled tiller bud growth and root development by regulating cytokinins content and cell cycle in plant cells. Altered expression of OsNPF7.2 also was responsible for the change in expression of the genes involved in strigolactone pathway, such as D27, D17, D10, Os900, Os1400, D14, D3, and OsFC1. Conclusion Our results suggested that OsNPF7.2 is a positive regulator of nitrate influx and concentration, and that it also regulates cell division in tiller bud and alters expression of genes involved in cytokinin and strigolactone pathways, resulting in the control over rice tiller number. Since elevated expression of OsNPF7.2 is capable of improving rice grain yield, this gene might be applied to high-yield rice breeding. |
| first_indexed | 2024-04-11T22:51:30Z |
| format | Article |
| id | doaj.art-2683de6c4d854907bedbaa62ef3ff6d2 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-11T22:51:30Z |
| publishDate | 2018-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-2683de6c4d854907bedbaa62ef3ff6d22022-12-22T03:58:34ZengSpringerOpenRice1939-84251939-84332018-02-0111111310.1186/s12284-018-0205-6Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yieldJie Wang0Kai Lu1Haipeng Nie2Qisen Zeng3Bowen Wu4Junjie Qian5Zhongming Fang6Center of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringCenter of Applied Biotechnology, Wuhan Institute of BioengineeringAbstract Background Rice tiller number is one of the most important factors that determine grain yield, while nitrogen is essential for the crop growth and development, especially for tiller formation. Genes involved in nitrogen use efficiency processes have been identified in the previous studies, however, only a small number of these genes have been found to improve grain yield by promoting tillering. Results We constructed over-expression (OX) lines and RNA-interference (Ri) lines, and selected a mutant of OsNPF7.2, a low-affinity nitrate transporter. Our analyses showed that rice tiller number and grain yield were significantly increased in OX lines, whereas Ri lines and mutant osnpf7.2 had fewer tiller number and lower grain yield. Under different nitrate concentrations, tiller buds grew faster in OX lines than in WT, but they grew slower in Ri lines and mutant osnpf7.2. These results indicated that altered expression of OsNPF7.2 plays a significant role in the control of tiller bud growth and regulation of tillering. Elevated expression of OsNPF7.2 also improved root length, root number, fresh weight, and dry weight. However, reduced expression of OsNPF7.2 had the opposite result on these characters. OsNPF7.2 OX lines showed more significantly enhanced influx of nitrate and had a higher nitrate concentration than WT. The levels of gene transcripts related to cytokinin pathway and cell cycle in tiller bud, and cytokinins concentration in tiller basal portion were higher in OX lines than that in WT, suggesting that altered expression of OsNPF7.2 controlled tiller bud growth and root development by regulating cytokinins content and cell cycle in plant cells. Altered expression of OsNPF7.2 also was responsible for the change in expression of the genes involved in strigolactone pathway, such as D27, D17, D10, Os900, Os1400, D14, D3, and OsFC1. Conclusion Our results suggested that OsNPF7.2 is a positive regulator of nitrate influx and concentration, and that it also regulates cell division in tiller bud and alters expression of genes involved in cytokinin and strigolactone pathways, resulting in the control over rice tiller number. Since elevated expression of OsNPF7.2 is capable of improving rice grain yield, this gene might be applied to high-yield rice breeding.http://link.springer.com/article/10.1186/s12284-018-0205-6RiceOsNPF7.2Tiller budCytokininTiller numberGrain yield |
| spellingShingle | Jie Wang Kai Lu Haipeng Nie Qisen Zeng Bowen Wu Junjie Qian Zhongming Fang Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield Rice Rice OsNPF7.2 Tiller bud Cytokinin Tiller number Grain yield |
| title | Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield |
| title_full | Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield |
| title_fullStr | Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield |
| title_full_unstemmed | Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield |
| title_short | Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield |
| title_sort | rice nitrate transporter osnpf7 2 positively regulates tiller number and grain yield |
| topic | Rice OsNPF7.2 Tiller bud Cytokinin Tiller number Grain yield |
| url | http://link.springer.com/article/10.1186/s12284-018-0205-6 |
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