MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL.
Intro: Community and hospital-acquired strains of Methicillin-resistant Staphylococcus aureus (MRSA) continue to pose a threat worldwide. Molecular epidemiological studies conducted worldwide continue to demonstrate shifts in predominantly circulating MRSA clones peculiar to their centres or countri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223005866 |
| _version_ | 1797745241460047872 |
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| author | U. Salamah Faisal M.N. Abdul Samat N. Hui Min N.A. Ahmad Zaidi U.A. Zainulabid S.A. Salleh |
| author_facet | U. Salamah Faisal M.N. Abdul Samat N. Hui Min N.A. Ahmad Zaidi U.A. Zainulabid S.A. Salleh |
| author_sort | U. Salamah Faisal |
| collection | DOAJ |
| description | Intro: Community and hospital-acquired strains of Methicillin-resistant Staphylococcus aureus (MRSA) continue to pose a threat worldwide. Molecular epidemiological studies conducted worldwide continue to demonstrate shifts in predominantly circulating MRSA clones peculiar to their centres or countries that have the propensity to cause infection in hospitals and the community. We aimed to detect our hospital's predominantly circulating MRSA clonal strain in 2016 and 2018 by spa typing to compare with predominant clones reported previously in our and other Malaysian hospitals. Methods: We conducted spa typing on 58 randomly selected, non-repetitive confirmed MRSA isolates collected from bloodstream infections in Hospital Canselor Tuanku Muhriz (HCTM) from 2016 (37 isolates) and 2018 (21 isolates). Extracted and purified DNA target sequences were amplified and sequenced using the method and primers previously described. The gene fragments were analysed using Ridom StaphType software to assign the spa types. We compared the clonal strains by spa type with local MRSA clones reported previously. Findings: The predominant MRSA clonal strain isolated was spa-type t032 in 2016 (83.8%, n=31) and 2018 (76.2%, n=16) from our hospital, identified in the literature as the European invasive strain. The remaining isolates detected were spa-type t020, t670, t022 and t304 (from 2016) and t189, t304, t315, t701 and t718 (from 2018). We did not detect spa-type t037 among the isolates, reported previously as the predominant MRSA spa type between 2009 and 2013 in several other Malaysian hospitals, including ours. Discussion: This preliminary data suggests that the predominantly circulating MRSA clonal strains have changed throughout the recent decade in Malaysian hospitals, likely contributed by mechanisms promoting their sustainability and ability to replace other strains, besides hospital infection control factors. Conclusion: Further investigation is warranted to elucidate these unknown mechanisms in light of the continuing evolution of MRSA strains and continued surveillance of genotypes, virulence factors and antimicrobial susceptibility patterns. |
| first_indexed | 2024-03-12T15:21:31Z |
| format | Article |
| id | doaj.art-2684a51d598b44dcb7b2489eed30b616 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-12T15:21:31Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-2684a51d598b44dcb7b2489eed30b6162023-08-11T05:32:14ZengElsevierInternational Journal of Infectious Diseases1201-97122023-08-01134S6S7MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL.U. Salamah Faisal0M.N. Abdul Samat1N. Hui Min2N.A. Ahmad Zaidi3U.A. Zainulabid4S.A. Salleh5National Defence University of Malaysia, Medical Faculty and Defense Health, Kuala Lumpur, MalaysiaHospital Canselor Tuanku Muhriz UKM, Microbiology and Immunology, Cheras Perdana, MalaysiaUKM Medical Molecular Biology Institute (UMBI), Molecular Microbiology & Infectious Diseases, Cheras, MalaysiaNational Defence University of Malaysia, Medical Faculty and Defense Health, Kuala Lumpur, MalaysiaInternational Islamic University Malaysia, Internal Medicine, Kulliyyah of Medicine, Kuantan, Malaysia; Hospital Canselor Tuanku Muhriz UKM, Internal Medicine, Cheras Perdana, MalaysiaHospital Canselor Tuanku Muhriz UKM, Microbiology and Immunology, Cheras Perdana, MalaysiaIntro: Community and hospital-acquired strains of Methicillin-resistant Staphylococcus aureus (MRSA) continue to pose a threat worldwide. Molecular epidemiological studies conducted worldwide continue to demonstrate shifts in predominantly circulating MRSA clones peculiar to their centres or countries that have the propensity to cause infection in hospitals and the community. We aimed to detect our hospital's predominantly circulating MRSA clonal strain in 2016 and 2018 by spa typing to compare with predominant clones reported previously in our and other Malaysian hospitals. Methods: We conducted spa typing on 58 randomly selected, non-repetitive confirmed MRSA isolates collected from bloodstream infections in Hospital Canselor Tuanku Muhriz (HCTM) from 2016 (37 isolates) and 2018 (21 isolates). Extracted and purified DNA target sequences were amplified and sequenced using the method and primers previously described. The gene fragments were analysed using Ridom StaphType software to assign the spa types. We compared the clonal strains by spa type with local MRSA clones reported previously. Findings: The predominant MRSA clonal strain isolated was spa-type t032 in 2016 (83.8%, n=31) and 2018 (76.2%, n=16) from our hospital, identified in the literature as the European invasive strain. The remaining isolates detected were spa-type t020, t670, t022 and t304 (from 2016) and t189, t304, t315, t701 and t718 (from 2018). We did not detect spa-type t037 among the isolates, reported previously as the predominant MRSA spa type between 2009 and 2013 in several other Malaysian hospitals, including ours. Discussion: This preliminary data suggests that the predominantly circulating MRSA clonal strains have changed throughout the recent decade in Malaysian hospitals, likely contributed by mechanisms promoting their sustainability and ability to replace other strains, besides hospital infection control factors. Conclusion: Further investigation is warranted to elucidate these unknown mechanisms in light of the continuing evolution of MRSA strains and continued surveillance of genotypes, virulence factors and antimicrobial susceptibility patterns.http://www.sciencedirect.com/science/article/pii/S1201971223005866 |
| spellingShingle | U. Salamah Faisal M.N. Abdul Samat N. Hui Min N.A. Ahmad Zaidi U.A. Zainulabid S.A. Salleh MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. International Journal of Infectious Diseases |
| title | MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. |
| title_full | MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. |
| title_fullStr | MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. |
| title_full_unstemmed | MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. |
| title_short | MRSA SPA-TYPE T032 OVERTAKES MRSA SPA-TYPE T037 AS THE PREDOMINANT CLONAL STRAIN: PRELIMINARY FINDINGS FROM A MALAYSIAN TERTIARY HOSPITAL. |
| title_sort | mrsa spa type t032 overtakes mrsa spa type t037 as the predominant clonal strain preliminary findings from a malaysian tertiary hospital |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223005866 |
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