Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy
Background: There is a paucity of published data on the effect of TB chemotherapy on nitric oxide (NO) synthesis and metabolism in newly diagnosed and relapsed patients with or without multi-drug resistant tuberculosis (MDRTB). Methods: The pattern of NO response in 140 patients with pulmonary TB, i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=2;spage=139;epage=143;aulast=Butov |
| _version_ | 1819319428959961088 |
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| author | Dmytro O Butov Mikhail M Kuzhko Irina M Kalmykova Irina M Kuznetsova Tatyana S Butova Olena O Grinishina Olga A Maksimenko |
| author_facet | Dmytro O Butov Mikhail M Kuzhko Irina M Kalmykova Irina M Kuznetsova Tatyana S Butova Olena O Grinishina Olga A Maksimenko |
| author_sort | Dmytro O Butov |
| collection | DOAJ |
| description | Background: There is a paucity of published data on the effect of TB chemotherapy on nitric oxide (NO) synthesis and metabolism in newly diagnosed and relapsed patients with or without multi-drug resistant tuberculosis (MDRTB).
Methods: The pattern of NO response in 140 patients with pulmonary TB, including 74 with MDR-TB and 66 without MDR-TB has been studied and compared to the NO status of 30 healthy donors. Patients comprised those with newly diagnosed TB and recurrent or relapsed TB. The NO status was assessed by measuring inducible NO synthase (iNOS) and nitrites and nitrates levels. This was measured prior to treatment initiation and two months after the prescribed chemotherapy.
Results: Increased levels of NO indices were found in patients with tuberculosis when compared to healthy controls. After two months of chemotherapy a significant decrease in NO indicators was observed in the patients with TB, particularly in those without MDR-TB and newly diagnosed TB. The decline in NO activity was less prominent in patients with recurrent TB and MDR-TB, which suggests lower level of immunologic and reparative processes in such patients.
Conclusion: Changes in serum levels of nitrites and nitrates as well as iNOS activity in neutrophils may serve as diagnostic criteria to differentiate various clinical forms of TB and help as prognostic tool to predict treatment outcome. |
| first_indexed | 2024-12-24T11:03:32Z |
| format | Article |
| id | doaj.art-2684db9af25f4d478d841fc42e72279c |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-24T11:03:32Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-2684db9af25f4d478d841fc42e72279c2022-12-21T16:58:40ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2014-01-013213914310.1016/j.ijmyco.2014.02.003Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapyDmytro O ButovMikhail M KuzhkoIrina M KalmykovaIrina M KuznetsovaTatyana S ButovaOlena O GrinishinaOlga A MaksimenkoBackground: There is a paucity of published data on the effect of TB chemotherapy on nitric oxide (NO) synthesis and metabolism in newly diagnosed and relapsed patients with or without multi-drug resistant tuberculosis (MDRTB). Methods: The pattern of NO response in 140 patients with pulmonary TB, including 74 with MDR-TB and 66 without MDR-TB has been studied and compared to the NO status of 30 healthy donors. Patients comprised those with newly diagnosed TB and recurrent or relapsed TB. The NO status was assessed by measuring inducible NO synthase (iNOS) and nitrites and nitrates levels. This was measured prior to treatment initiation and two months after the prescribed chemotherapy. Results: Increased levels of NO indices were found in patients with tuberculosis when compared to healthy controls. After two months of chemotherapy a significant decrease in NO indicators was observed in the patients with TB, particularly in those without MDR-TB and newly diagnosed TB. The decline in NO activity was less prominent in patients with recurrent TB and MDR-TB, which suggests lower level of immunologic and reparative processes in such patients. Conclusion: Changes in serum levels of nitrites and nitrates as well as iNOS activity in neutrophils may serve as diagnostic criteria to differentiate various clinical forms of TB and help as prognostic tool to predict treatment outcome.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=2;spage=139;epage=143;aulast=ButovPulmonary tuberculosisMDR-TBMTBNitric oxideNOInducible NO synthase |
| spellingShingle | Dmytro O Butov Mikhail M Kuzhko Irina M Kalmykova Irina M Kuznetsova Tatyana S Butova Olena O Grinishina Olga A Maksimenko Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy International Journal of Mycobacteriology Pulmonary tuberculosis MDR-TB MTB Nitric oxide NO Inducible NO synthase |
| title | Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy |
| title_full | Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy |
| title_fullStr | Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy |
| title_full_unstemmed | Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy |
| title_short | Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy |
| title_sort | changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without mdr tb undergoing the intensive phase of anti tuberculosis therapy |
| topic | Pulmonary tuberculosis MDR-TB MTB Nitric oxide NO Inducible NO synthase |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=2;spage=139;epage=143;aulast=Butov |
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