Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte
Abstract Purpose To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs). Methods HCKs and BALB/c mice were chosen as the study subject. Ethanol was used to treat the cells and corneal stroma of mice to build the ethanol injured model in vitro a...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-01-01
|
| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12886-022-02255-8 |
| _version_ | 1798025203924598784 |
|---|---|
| author | Jinghua Liu Chen Guo Peng Hao Peihong Wang Linghan Li Yuchuan Wang Xuan Li |
| author_facet | Jinghua Liu Chen Guo Peng Hao Peihong Wang Linghan Li Yuchuan Wang Xuan Li |
| author_sort | Jinghua Liu |
| collection | DOAJ |
| description | Abstract Purpose To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs). Methods HCKs and BALB/c mice were chosen as the study subject. Ethanol was used to treat the cells and corneal stroma of mice to build the ethanol injured model in vitro and vivo respectively. CCK-8 was used to evaluate the cell metabolic activity. DCFH-DA was used to detect the intracellular reactive oxygen species level. TUNEL was chose to detect the cell apoptosis rate. The cell proliferation and migration were investigated by using wound healing insert. Wound healing of corneal surface and stroma was observed by using fluorescein sodium eyedrop and HE stain. RT-qPCR, ELISA, and immunostaining were performed to detect gene and protein expression in keratocytes or corneal stroma tissue of mice. Results Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3. Tβ4 promotes the proliferation of HCKs and the process of corneal wound healing. It may relevant to the up-regulated expression of Ki67. Conclusions Our study established an ethanol-injured corneal stroma model in both vitro and vivo. The present study confirmed that Tβ4 play a protective effect on the reconstruction process of ethanol-injured corneal stroma. |
| first_indexed | 2024-04-11T18:14:58Z |
| format | Article |
| id | doaj.art-2685bd516b6f44d5b214c23e4ab6bc6a |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-04-11T18:14:58Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-2685bd516b6f44d5b214c23e4ab6bc6a2022-12-22T04:09:56ZengBMCBMC Ophthalmology1471-24152022-01-0122111010.1186/s12886-022-02255-8Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyteJinghua Liu0Chen Guo1Peng Hao2Peihong Wang3Linghan Li4Yuchuan Wang5Xuan Li6Nankai UniversityTianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Tianjin Medical University Eye Hospital, Eye Institute and School of OptometryNankai University Eye HospitalTianjin Key Laboratory of Ophthalmology and Visual Science, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Eye Hospital, Tianjin Eye InstituteShanxi Eye Hospital, Xi’an People’s Hospital, Xi’an Fourth HospitalNankai University Eye HospitalNankai University Eye HospitalAbstract Purpose To investigate the protective effects of thymosin β4 (Tβ4) on ethanol injured human corneal keratocytes (HCKs). Methods HCKs and BALB/c mice were chosen as the study subject. Ethanol was used to treat the cells and corneal stroma of mice to build the ethanol injured model in vitro and vivo respectively. CCK-8 was used to evaluate the cell metabolic activity. DCFH-DA was used to detect the intracellular reactive oxygen species level. TUNEL was chose to detect the cell apoptosis rate. The cell proliferation and migration were investigated by using wound healing insert. Wound healing of corneal surface and stroma was observed by using fluorescein sodium eyedrop and HE stain. RT-qPCR, ELISA, and immunostaining were performed to detect gene and protein expression in keratocytes or corneal stroma tissue of mice. Results Ethanol induced oxidative stress injury and cell apoptosis on HCKs, and Tβ4 can alleviate it by up-regulating the expression of Bcl-2, catalase, and CuZnSOD, and inhibiting the expression of Caspase-3. Tβ4 promotes the proliferation of HCKs and the process of corneal wound healing. It may relevant to the up-regulated expression of Ki67. Conclusions Our study established an ethanol-injured corneal stroma model in both vitro and vivo. The present study confirmed that Tβ4 play a protective effect on the reconstruction process of ethanol-injured corneal stroma.https://doi.org/10.1186/s12886-022-02255-8Thymosin β4Cornea stromaEthanolOxidative stressApoptosis |
| spellingShingle | Jinghua Liu Chen Guo Peng Hao Peihong Wang Linghan Li Yuchuan Wang Xuan Li Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte BMC Ophthalmology Thymosin β4 Cornea stroma Ethanol Oxidative stress Apoptosis |
| title | Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| title_full | Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| title_fullStr | Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| title_full_unstemmed | Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| title_short | Protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| title_sort | protection effect of thymosin β4 on ethanol injury in corneal stromal keratocyte |
| topic | Thymosin β4 Cornea stroma Ethanol Oxidative stress Apoptosis |
| url | https://doi.org/10.1186/s12886-022-02255-8 |
| work_keys_str_mv | AT jinghualiu protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT chenguo protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT penghao protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT peihongwang protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT linghanli protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT yuchuanwang protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte AT xuanli protectioneffectofthymosinb4onethanolinjuryincornealstromalkeratocyte |