Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol
Introduction: Potassium dichromate, a chromium {Cr(VI)} compound is the most toxic form of Cr(VI) which causes nephrotoxicity associated with oxidative stress in animals and humans. The study of toxicity and biological effects associated with chromium has generated a lot of interest due to its w...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2021-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/15645/50039_CE[Ra1]_F[SH]_GC(AnK)_PF1(MG_SS)_PFA(MG_KM)_PN(KM).pdf |
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| author | Nahid Yasmin Md Sabir Hussain |
| author_facet | Nahid Yasmin Md Sabir Hussain |
| author_sort | Nahid Yasmin |
| collection | DOAJ |
| description | Introduction: Potassium dichromate, a chromium {Cr(VI)} compound
is the most toxic form of Cr(VI) which causes nephrotoxicity
associated with oxidative stress in animals and humans. The
study of toxicity and biological effects associated with chromium
has generated a lot of interest due to its wider distribution in the
environment and its use.
Aim: To study the potassium dichromate-induced nephrotoxicity
and ameliorative role of vitamin E in albino rats.
Materials and Methods: The present experimental study was
conducted in the Department of Anatomy, Subharti Medical College,
Meerut, Uttar Pradesh, India. Study population consisted of 36 albino
rats which were randomly divided into four equal groups (n=9, each).
Group I served as control while groups II and III were administered
Potassium dichromate (K2
Cr2
O7
) dissolved in sterile distilled water
10 mg/kg body weight single dose orally for 1, 14 and 42 days, 3 rats
in each sub-groups. In addition to Cr(VI), group III also received
α-tocopherol 125 mg/kg body weight daily orally. Group IV rats were
maintained as α-tocopherol control and they received α-tocopherol
as above daily for 42 days. At the end of 1, 14 and 42 days, all the rats
were sacrificed for the estimation of the histopathological changes in
kidney. The Z-proportion test was used for evaluation of data.
Results: Thirt-six albino rats of both sex, age about 60 days
weighing approximately 140±10 gm were studied. Prominent
pathological changes like glomerular mesangial proliferation
and obliteration of bowman’s space were observed in the
kidney of group II rats. Co-treatment with α-tocopherol in
group III significantly reversed the Cr(VI) induced changes with
a p-value=0.009 and 0.007 for glomerular mesangial proliferation
and obliteration of bowman’s space respectively.
Conclusion: The α-tocopherol exhibited protective effect against
Cr(VI) induced damage to the kidney. |
| first_indexed | 2024-12-21T23:52:03Z |
| format | Article |
| id | doaj.art-2685d82d1d764b148f05b9bc094234fb |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T23:52:03Z |
| publishDate | 2021-11-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-2685d82d1d764b148f05b9bc094234fb2022-12-21T18:45:55ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-11-011511AC05AC0810.7860/JCDR/2021/50039.15645Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-TocopherolNahid Yasmin0Md Sabir Hussain1Assistant Professor, Department of Anatomy, Mahatma Gandhi Medical College, Jaipur, Rajasthan, India.Assistant Professor, Department of Physiology, Mahatma Gandhi Medical College, Jaipur, Rajasthan, India.Introduction: Potassium dichromate, a chromium {Cr(VI)} compound is the most toxic form of Cr(VI) which causes nephrotoxicity associated with oxidative stress in animals and humans. The study of toxicity and biological effects associated with chromium has generated a lot of interest due to its wider distribution in the environment and its use. Aim: To study the potassium dichromate-induced nephrotoxicity and ameliorative role of vitamin E in albino rats. Materials and Methods: The present experimental study was conducted in the Department of Anatomy, Subharti Medical College, Meerut, Uttar Pradesh, India. Study population consisted of 36 albino rats which were randomly divided into four equal groups (n=9, each). Group I served as control while groups II and III were administered Potassium dichromate (K2 Cr2 O7 ) dissolved in sterile distilled water 10 mg/kg body weight single dose orally for 1, 14 and 42 days, 3 rats in each sub-groups. In addition to Cr(VI), group III also received α-tocopherol 125 mg/kg body weight daily orally. Group IV rats were maintained as α-tocopherol control and they received α-tocopherol as above daily for 42 days. At the end of 1, 14 and 42 days, all the rats were sacrificed for the estimation of the histopathological changes in kidney. The Z-proportion test was used for evaluation of data. Results: Thirt-six albino rats of both sex, age about 60 days weighing approximately 140±10 gm were studied. Prominent pathological changes like glomerular mesangial proliferation and obliteration of bowman’s space were observed in the kidney of group II rats. Co-treatment with α-tocopherol in group III significantly reversed the Cr(VI) induced changes with a p-value=0.009 and 0.007 for glomerular mesangial proliferation and obliteration of bowman’s space respectively. Conclusion: The α-tocopherol exhibited protective effect against Cr(VI) induced damage to the kidney.https://jcdr.net/articles/PDF/15645/50039_CE[Ra1]_F[SH]_GC(AnK)_PF1(MG_SS)_PFA(MG_KM)_PN(KM).pdfglomerular mesangial proliferationobliterated bowman’s spacepotassium dichromatevitamin e |
| spellingShingle | Nahid Yasmin Md Sabir Hussain Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol Journal of Clinical and Diagnostic Research glomerular mesangial proliferation obliterated bowman’s space potassium dichromate vitamin e |
| title | Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol |
| title_full | Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol |
| title_fullStr | Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol |
| title_full_unstemmed | Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol |
| title_short | Histopathological Changes in the Kidney of Albino Rat due to Chromium and the Ameliorative Role of α-Tocopherol |
| title_sort | histopathological changes in the kidney of albino rat due to chromium and the ameliorative role of α tocopherol |
| topic | glomerular mesangial proliferation obliterated bowman’s space potassium dichromate vitamin e |
| url | https://jcdr.net/articles/PDF/15645/50039_CE[Ra1]_F[SH]_GC(AnK)_PF1(MG_SS)_PFA(MG_KM)_PN(KM).pdf |
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