Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria
Morphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained <i>Globodera pal...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/10/2/216 |
| _version_ | 1797396371066585088 |
|---|---|
| author | Aouicha Djebroune Gahdab Chakali Eugénia de Andrade Maria João Camacho Leidy Rusinque Maria L. Inácio |
| author_facet | Aouicha Djebroune Gahdab Chakali Eugénia de Andrade Maria João Camacho Leidy Rusinque Maria L. Inácio |
| author_sort | Aouicha Djebroune |
| collection | DOAJ |
| description | Morphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained <i>Globodera pallida</i> alone, 28% contained <i>Globodera rostochiensis</i> alone and 28% mixtures of the two species. Morphometric values of cysts and second-stage juveniles were generally distributed with slight differences in the expected ranges for both <i>Globodera</i> species. Inter- and intraspecific morphometric variability in nematode isolates was noted. Molecular analysis using conventional multiplex PCR with species-specific primers and TaqMan real-time PCR confirmed the morphological identification. In addition, the distribution of both potato cyst nematode species throughout various parts of the country was investigated. In the central areas, the isolates of <i>G. pallida</i> alone dominate, whereas isolates of <i>G. rostochiensis</i> alone are more frequent in the southern areas. In the eastern regions, mixed isolates are more representative. Most isolates examined in the western areas are mixtures of the two species or <i>G. rostochiensis</i> alone. Comparatively, <i>G. pallida</i> remains the most widely distributed species in its geographic range. This study confirms the presence of two PCN species, <i>G. pallida</i> and <i>G. rostochiensis</i>, in Algeria and provides additional information on their biogeographic distribution. |
| first_indexed | 2024-03-09T00:49:19Z |
| format | Article |
| id | doaj.art-268e77081cd644eaba87967f9d81407c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:49:19Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-268e77081cd644eaba87967f9d81407c2023-12-11T17:16:11ZengMDPI AGPathogens2076-08172021-02-0110221610.3390/pathogens10020216Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in AlgeriaAouicha Djebroune0Gahdab Chakali1Eugénia de Andrade2Maria João Camacho3Leidy Rusinque4Maria L. Inácio5Département des Sciences Agronomiques, Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre, Université Djilali Bounaama Khemis Miliana, Route de Theniet El Had, 44225 Ain Defla, AlgeriaDépartement de Zoologie Agricole et Forestière, Ecole Nationale Supérieure Agronomique, El-Harrach, 16200 Algiers, AlgeriaInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalMorphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained <i>Globodera pallida</i> alone, 28% contained <i>Globodera rostochiensis</i> alone and 28% mixtures of the two species. Morphometric values of cysts and second-stage juveniles were generally distributed with slight differences in the expected ranges for both <i>Globodera</i> species. Inter- and intraspecific morphometric variability in nematode isolates was noted. Molecular analysis using conventional multiplex PCR with species-specific primers and TaqMan real-time PCR confirmed the morphological identification. In addition, the distribution of both potato cyst nematode species throughout various parts of the country was investigated. In the central areas, the isolates of <i>G. pallida</i> alone dominate, whereas isolates of <i>G. rostochiensis</i> alone are more frequent in the southern areas. In the eastern regions, mixed isolates are more representative. Most isolates examined in the western areas are mixtures of the two species or <i>G. rostochiensis</i> alone. Comparatively, <i>G. pallida</i> remains the most widely distributed species in its geographic range. This study confirms the presence of two PCN species, <i>G. pallida</i> and <i>G. rostochiensis</i>, in Algeria and provides additional information on their biogeographic distribution.https://www.mdpi.com/2076-0817/10/2/216potato cyst nematodesmorphologyPCRgeographical distributionAlgeria |
| spellingShingle | Aouicha Djebroune Gahdab Chakali Eugénia de Andrade Maria João Camacho Leidy Rusinque Maria L. Inácio Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria Pathogens potato cyst nematodes morphology PCR geographical distribution Algeria |
| title | Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria |
| title_full | Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria |
| title_fullStr | Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria |
| title_full_unstemmed | Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria |
| title_short | Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes <i>Globodera rostochiensis</i> and <i>Globodera pallida</i> (Tylenchida: Heteroderidae) in Algeria |
| title_sort | integrative morphometric and molecular approach to update the impact and distribution of potato cyst nematodes i globodera rostochiensis i and i globodera pallida i tylenchida heteroderidae in algeria |
| topic | potato cyst nematodes morphology PCR geographical distribution Algeria |
| url | https://www.mdpi.com/2076-0817/10/2/216 |
| work_keys_str_mv | AT aouichadjebroune integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria AT gahdabchakali integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria AT eugeniadeandrade integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria AT mariajoaocamacho integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria AT leidyrusinque integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria AT marialinacio integrativemorphometricandmolecularapproachtoupdatetheimpactanddistributionofpotatocystnematodesigloboderarostochiensisiandigloboderapallidaitylenchidaheteroderidaeinalgeria |