Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage

Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage

Probiotics are typically enumerated by agar plate counting (PC) techniques. PC has several limitations including poor specificity, high variability, inability to enumerate dead cells, viable but non-culturable cells and cells in complex matrices. Viability droplet digital polymerase chain reaction (...

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Bibliographic Details
Main Authors: Anthony Kiefer, Phillip M. Byrd, Peipei Tang, Gregory Jones, Kevin Galles, Vincenzo Fallico, Connie Wong
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Microbiology
Subjects:
probiotic
storage
v-ddPCR
viability
enumeration
PMA
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.966264/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.966264/full

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