Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i>
<i>Anoxybacillus amylolyticus</i> is a moderate thermophilic microorganism producing an exopolysaccharide and an extracellular α-amylase able to hydrolyze starch. The synthesis of several biomolecules is often regulated by a quorum sensing (QS) mechanism, a chemical cell-to-cell communic...
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/4/819 |
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| author | Annabella Tramice Adele Cutignano Annalaura Iodice Annarita Poli Ilaria Finore Giuseppina Tommonaro |
| author_facet | Annabella Tramice Adele Cutignano Annalaura Iodice Annarita Poli Ilaria Finore Giuseppina Tommonaro |
| author_sort | Annabella Tramice |
| collection | DOAJ |
| description | <i>Anoxybacillus amylolyticus</i> is a moderate thermophilic microorganism producing an exopolysaccharide and an extracellular α-amylase able to hydrolyze starch. The synthesis of several biomolecules is often regulated by a quorum sensing (QS) mechanism, a chemical cell-to-cell communication based on the production and diffusion of small molecules named “autoinducers”, most of which belonging to the <i>N</i>-acyl homoserine lactones’ (AHLs) family. There are few reports about this mechanism in extremophiles, in particular thermophiles. Here, we report the identification of a signal molecule, the <i>N</i>-butanoyl-homoserine lactone (C4-HSL), from the milieu of <i>A. amylolyticus</i>. Moreover, investigations performed by supplementing a known QS inhibitor, <i>trans</i>-cinnamaldehyde, or exogenous C4-HSL in the growth medium of <i>A. amylolyticus</i> suggested the involvement of QS signaling in the modulation of extracellular α-amylase activity. The data showed that the presence of the QS inhibitor <i>trans</i>-cinnamaldehyde in the medium decreased amylolytic activity, which, conversely, was increased by the effect of exogenous C4-HSL. Overall, these results represent the first evidence of the production of AHLs in thermophilic microorganisms, which could be responsible for a communication system regulating thermostable α-amylase activity. |
| first_indexed | 2024-03-10T12:21:56Z |
| format | Article |
| id | doaj.art-2691629d1b604e4b9831ece240523a00 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:21:56Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2691629d1b604e4b9831ece240523a002023-11-21T15:21:26ZengMDPI AGMicroorganisms2076-26072021-04-019481910.3390/microorganisms9040819Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i>Annabella Tramice0Adele Cutignano1Annalaura Iodice2Annarita Poli3Ilaria Finore4Giuseppina Tommonaro5National Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, ItalyNational Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, ItalyNational Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, ItalyNational Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, ItalyNational Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, ItalyNational Research Council of Italy, Institute of Biomolecular Chemistry, 34- 80078 Pozzuoli, Italy<i>Anoxybacillus amylolyticus</i> is a moderate thermophilic microorganism producing an exopolysaccharide and an extracellular α-amylase able to hydrolyze starch. The synthesis of several biomolecules is often regulated by a quorum sensing (QS) mechanism, a chemical cell-to-cell communication based on the production and diffusion of small molecules named “autoinducers”, most of which belonging to the <i>N</i>-acyl homoserine lactones’ (AHLs) family. There are few reports about this mechanism in extremophiles, in particular thermophiles. Here, we report the identification of a signal molecule, the <i>N</i>-butanoyl-homoserine lactone (C4-HSL), from the milieu of <i>A. amylolyticus</i>. Moreover, investigations performed by supplementing a known QS inhibitor, <i>trans</i>-cinnamaldehyde, or exogenous C4-HSL in the growth medium of <i>A. amylolyticus</i> suggested the involvement of QS signaling in the modulation of extracellular α-amylase activity. The data showed that the presence of the QS inhibitor <i>trans</i>-cinnamaldehyde in the medium decreased amylolytic activity, which, conversely, was increased by the effect of exogenous C4-HSL. Overall, these results represent the first evidence of the production of AHLs in thermophilic microorganisms, which could be responsible for a communication system regulating thermostable α-amylase activity.https://www.mdpi.com/2076-2607/9/4/819<i>Anoxybacillus amylolyticus</i>extremophilesquorum sensingα-amylasetrans-cinnamaldehydeUPLC/MRM |
| spellingShingle | Annabella Tramice Adele Cutignano Annalaura Iodice Annarita Poli Ilaria Finore Giuseppina Tommonaro Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> Microorganisms <i>Anoxybacillus amylolyticus</i> extremophiles quorum sensing α-amylase trans-cinnamaldehyde UPLC/MRM |
| title | Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> |
| title_full | Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> |
| title_fullStr | Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> |
| title_full_unstemmed | Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> |
| title_short | Involvement of a Quorum Sensing Signal Molecule in the Extracellular Amylase Activity of the Thermophilic <i>Anoxybacillus amylolyticus</i> |
| title_sort | involvement of a quorum sensing signal molecule in the extracellular amylase activity of the thermophilic i anoxybacillus amylolyticus i |
| topic | <i>Anoxybacillus amylolyticus</i> extremophiles quorum sensing α-amylase trans-cinnamaldehyde UPLC/MRM |
| url | https://www.mdpi.com/2076-2607/9/4/819 |
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