Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease
Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic <i>Escherichia coli</i>, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clar...
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MDPI AG
2020-12-01
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| author | Anca Mare Adrian Man Felicia Toma Cristina Nicoleta Ciurea Răzvan Lucian Coșeriu Camelia Vintilă Adrian Cornel Maier |
| author_facet | Anca Mare Adrian Man Felicia Toma Cristina Nicoleta Ciurea Răzvan Lucian Coșeriu Camelia Vintilă Adrian Cornel Maier |
| author_sort | Anca Mare |
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| description | Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic <i>Escherichia coli</i>, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mureș County Hospital, between May 2016 and July 2019, 160 diarrheagenic <i>E. coli</i> strains were isolated from children under 2 years old with diarrheic disease. The strains were identified as Shiga toxin-producing <i>E. coli</i> (STEC)/enteropathogenic <i>Escherichia coli</i> (EPEC) via agglutination with polyvalent sera. STEC strains were serotyped using monovalent sera for serogroup O157. Simplex PCR was performed on the strains to determine the presence of the <i>hlyA</i> gene, and, for the positive ones, the hemolytic activity was tested. Antibiotic susceptibility of the identified diarrheagenic <i>E. coli</i> strains was also investigated. STEC strains were the most frequently identified (49.1%), followed by EPEC (40.2%). The <i>hly</i>A gene was identified in 12 cases, representing 18.2% of the STEC strains. Even if the extended-spectrum β-lactamase (ESBL)-producing strains represented only 10%, a relevant percentage of multidrug-resistant (MDR) strains (24%) was identified. |
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| series | Pathogens |
| spelling | doaj.art-2698c87657d24916a9c48809f0fa10d82023-11-20T23:28:43ZengMDPI AGPathogens2076-08172020-12-01912102210.3390/pathogens9121022Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal DiseaseAnca Mare0Adrian Man1Felicia Toma2Cristina Nicoleta Ciurea3Răzvan Lucian Coșeriu4Camelia Vintilă5Adrian Cornel Maier6Department of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 Târgu Mureș, RomaniaDepartment of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 Târgu Mureș, RomaniaDepartment of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 Târgu Mureș, RomaniaDepartment of Microbiology, Pharmacy, Sciences and Technology, George Emil Palade University of Medicine, 540142 Târgu Mureș, RomaniaInfectious Diseases Laboratory, Mureș County Clinical Hospital, 540233 Târgu Mureș, RomaniaInfectious Diseases Laboratory, Mureș County Clinical Hospital, 540233 Târgu Mureș, RomaniaDepartment of Urology, Faculty of Medicine, University “Dunarea de Jos” Galați, 800008 Galați, RomaniaEven if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic <i>Escherichia coli</i>, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mureș County Hospital, between May 2016 and July 2019, 160 diarrheagenic <i>E. coli</i> strains were isolated from children under 2 years old with diarrheic disease. The strains were identified as Shiga toxin-producing <i>E. coli</i> (STEC)/enteropathogenic <i>Escherichia coli</i> (EPEC) via agglutination with polyvalent sera. STEC strains were serotyped using monovalent sera for serogroup O157. Simplex PCR was performed on the strains to determine the presence of the <i>hlyA</i> gene, and, for the positive ones, the hemolytic activity was tested. Antibiotic susceptibility of the identified diarrheagenic <i>E. coli</i> strains was also investigated. STEC strains were the most frequently identified (49.1%), followed by EPEC (40.2%). The <i>hly</i>A gene was identified in 12 cases, representing 18.2% of the STEC strains. Even if the extended-spectrum β-lactamase (ESBL)-producing strains represented only 10%, a relevant percentage of multidrug-resistant (MDR) strains (24%) was identified.https://www.mdpi.com/2076-0817/9/12/1022diarrheagenic <i>Escherichia coli</i><i>hlyA</i>MDR |
| spellingShingle | Anca Mare Adrian Man Felicia Toma Cristina Nicoleta Ciurea Răzvan Lucian Coșeriu Camelia Vintilă Adrian Cornel Maier Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease Pathogens diarrheagenic <i>Escherichia coli</i> <i>hlyA</i> MDR |
| title | Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease |
| title_full | Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease |
| title_fullStr | Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease |
| title_full_unstemmed | Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease |
| title_short | Hemolysin-Producing Strains among Diarrheagenic <i>Escherichia coli</i> Isolated from Children under 2 Years Old with Diarrheal Disease |
| title_sort | hemolysin producing strains among diarrheagenic i escherichia coli i isolated from children under 2 years old with diarrheal disease |
| topic | diarrheagenic <i>Escherichia coli</i> <i>hlyA</i> MDR |
| url | https://www.mdpi.com/2076-0817/9/12/1022 |
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