POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation
POLD4 plays a crucial part in the complex machinery of DNA replication and repair as a vital component of the DNA polymerase delta complex. In this research, we obtained original information from various publicly available databases. Using a blend of R programming and internet resources, we initiate...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/18/13919 |
| _version_ | 1797579712731545600 |
|---|---|
| author | Cheng Jiang Fei Fan Weiming Xu Xiaobing Jiang |
| author_facet | Cheng Jiang Fei Fan Weiming Xu Xiaobing Jiang |
| author_sort | Cheng Jiang |
| collection | DOAJ |
| description | POLD4 plays a crucial part in the complex machinery of DNA replication and repair as a vital component of the DNA polymerase delta complex. In this research, we obtained original information from various publicly available databases. Using a blend of R programming and internet resources, we initiated an extensive examination into the correlation between POLD4 expression and the various elements of cancers. In addition, we performed knockdown experiments in glioma cell lines to authenticate its significant impact. We discovered that POLD4 is upregulated in various malignant tumors, demonstrating a significant correlation with poor patient survival prognosis. Using function analysis, it was uncovered that POLD4 exhibited intricate associations with signaling pathways spanning multiple tumor types. Subsequent investigations unveiled the close association of POLD4 with the immune microenvironment and the effectiveness of immunotherapy. Drugs like trametinib, saracatinib, and dasatinib may be used in patients with high POLD4. Using experimental analysis, we further confirmed the overexpression of POLD4 in gliomas, as well as its correlation with glioma recurrence, proliferation, and the suppressive immune microenvironment. Our research findings indicate that the expression pattern of POLD4 not only serves as a robust indicator of prognosis in cancer patients but also holds promising potential as a new focus for treatment. |
| first_indexed | 2024-03-10T22:40:02Z |
| format | Article |
| id | doaj.art-2699ce74a4574244ab08666ea8ee3bfc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:02Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2699ce74a4574244ab08666ea8ee3bfc2023-11-19T11:05:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181391910.3390/ijms241813919POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental ValidationCheng Jiang0Fei Fan1Weiming Xu2Xiaobing Jiang3Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, ChinaDepartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, ChinaDepartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, ChinaDepartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, ChinaPOLD4 plays a crucial part in the complex machinery of DNA replication and repair as a vital component of the DNA polymerase delta complex. In this research, we obtained original information from various publicly available databases. Using a blend of R programming and internet resources, we initiated an extensive examination into the correlation between POLD4 expression and the various elements of cancers. In addition, we performed knockdown experiments in glioma cell lines to authenticate its significant impact. We discovered that POLD4 is upregulated in various malignant tumors, demonstrating a significant correlation with poor patient survival prognosis. Using function analysis, it was uncovered that POLD4 exhibited intricate associations with signaling pathways spanning multiple tumor types. Subsequent investigations unveiled the close association of POLD4 with the immune microenvironment and the effectiveness of immunotherapy. Drugs like trametinib, saracatinib, and dasatinib may be used in patients with high POLD4. Using experimental analysis, we further confirmed the overexpression of POLD4 in gliomas, as well as its correlation with glioma recurrence, proliferation, and the suppressive immune microenvironment. Our research findings indicate that the expression pattern of POLD4 not only serves as a robust indicator of prognosis in cancer patients but also holds promising potential as a new focus for treatment.https://www.mdpi.com/1422-0067/24/18/13919DNA polymerase delta subunit 4 (POLD4)proliferationimmunosuppressive microenvironmentimmunomarkerpan-cancergliomas |
| spellingShingle | Cheng Jiang Fei Fan Weiming Xu Xiaobing Jiang POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation International Journal of Molecular Sciences DNA polymerase delta subunit 4 (POLD4) proliferation immunosuppressive microenvironment immunomarker pan-cancer gliomas |
| title | POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation |
| title_full | POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation |
| title_fullStr | POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation |
| title_full_unstemmed | POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation |
| title_short | POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune Microenvironment: A Pan-Cancer Analysis Integrated with Experimental Validation |
| title_sort | pold4 promotes glioma cell proliferation and suppressive immune microenvironment a pan cancer analysis integrated with experimental validation |
| topic | DNA polymerase delta subunit 4 (POLD4) proliferation immunosuppressive microenvironment immunomarker pan-cancer gliomas |
| url | https://www.mdpi.com/1422-0067/24/18/13919 |
| work_keys_str_mv | AT chengjiang pold4promotesgliomacellproliferationandsuppressiveimmunemicroenvironmentapancanceranalysisintegratedwithexperimentalvalidation AT feifan pold4promotesgliomacellproliferationandsuppressiveimmunemicroenvironmentapancanceranalysisintegratedwithexperimentalvalidation AT weimingxu pold4promotesgliomacellproliferationandsuppressiveimmunemicroenvironmentapancanceranalysisintegratedwithexperimentalvalidation AT xiaobingjiang pold4promotesgliomacellproliferationandsuppressiveimmunemicroenvironmentapancanceranalysisintegratedwithexperimentalvalidation |