Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires
Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/1/227 |
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| author | Damiano Verardo Leena Liljedahl Corinna Richter Björn Agnarsson Ulrika Axelsson Christelle N. Prinz Fredrik Höök Carl A. K. Borrebaeck Heiner Linke |
| author_facet | Damiano Verardo Leena Liljedahl Corinna Richter Björn Agnarsson Ulrika Axelsson Christelle N. Prinz Fredrik Höök Carl A. K. Borrebaeck Heiner Linke |
| author_sort | Damiano Verardo |
| collection | DOAJ |
| description | Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO<sub>2</sub>-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays. |
| first_indexed | 2024-03-09T04:33:40Z |
| format | Article |
| id | doaj.art-26a43d59beb24f4e9b90939e3db5454f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T04:33:40Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-26a43d59beb24f4e9b90939e3db5454f2023-12-03T13:31:18ZengMDPI AGNanomaterials2079-49912021-01-0111122710.3390/nano11010227Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding NanowiresDamiano Verardo0Leena Liljedahl1Corinna Richter2Björn Agnarsson3Ulrika Axelsson4Christelle N. Prinz5Fredrik Höök6Carl A. K. Borrebaeck7Heiner Linke8NanoLund, Lund University, Box 118, 22100 Lund, SwedenCREATE Health Translational Cancer Center, Department of Immunotechnology, Lund University, Medicon Village Bldg 406, 223 63 Lund, SwedenCREATE Health Translational Cancer Center, Department of Immunotechnology, Lund University, Medicon Village Bldg 406, 223 63 Lund, SwedenDepartment of Physics, Chalmers University of Technology, 41296 Gothenburg, SwedenCREATE Health Translational Cancer Center, Department of Immunotechnology, Lund University, Medicon Village Bldg 406, 223 63 Lund, SwedenNanoLund, Lund University, Box 118, 22100 Lund, SwedenNanoLund, Lund University, Box 118, 22100 Lund, SwedenCREATE Health Translational Cancer Center, Department of Immunotechnology, Lund University, Medicon Village Bldg 406, 223 63 Lund, SwedenNanoLund, Lund University, Box 118, 22100 Lund, SwedenFluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO<sub>2</sub>-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays.https://www.mdpi.com/2079-4991/11/1/227antibody microarraynanowire sensorsbiomarker discovery |
| spellingShingle | Damiano Verardo Leena Liljedahl Corinna Richter Björn Agnarsson Ulrika Axelsson Christelle N. Prinz Fredrik Höök Carl A. K. Borrebaeck Heiner Linke Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires Nanomaterials antibody microarray nanowire sensors biomarker discovery |
| title | Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires |
| title_full | Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires |
| title_fullStr | Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires |
| title_full_unstemmed | Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires |
| title_short | Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires |
| title_sort | fluorescence signal enhancement in antibody microarrays using lightguiding nanowires |
| topic | antibody microarray nanowire sensors biomarker discovery |
| url | https://www.mdpi.com/2079-4991/11/1/227 |
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