A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models
Abstract Background Bao-Gan-Xing-Jiu-Wan (BGXJW) is a clinical experience-based Chinese herbal formula. Its efficacy, pharmacological safety, targeted function, process quality, and other aspects have met the evaluation standards and the latest requirements of preparations. It could prevent and alle...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Chinese Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13020-023-00753-5 |
| _version_ | 1797831868330016768 |
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| author | Han Gao Zhen Li Yao Liu Yong-kang Zhao Cheng Cheng Feng Qiu Yuan Gao Ya-wen Lu Xin-hua Song Jia-bo Wang Zhi-tao Ma |
| author_facet | Han Gao Zhen Li Yao Liu Yong-kang Zhao Cheng Cheng Feng Qiu Yuan Gao Ya-wen Lu Xin-hua Song Jia-bo Wang Zhi-tao Ma |
| author_sort | Han Gao |
| collection | DOAJ |
| description | Abstract Background Bao-Gan-Xing-Jiu-Wan (BGXJW) is a clinical experience-based Chinese herbal formula. Its efficacy, pharmacological safety, targeted function, process quality, and other aspects have met the evaluation standards and the latest requirements of preparations. It could prevent and alleviate the symptoms of drunkenness and alcoholic liver injury clinically. The present work aims to elucidate whether BGXJW could protect against drunkenness and alcoholic liver disease in mice and explore the associated mechanism. Material and methods We used acute-on-chronic (NIAAA) mice model to induce alcoholic steatosis, and alcohol binge-drinking model to reappear the drunk condition. BGXJW at indicated doses were administered by oral gavage respectively to analyze its effects on alcoholic liver injury and the associated molecular mechanisms. Results BGXJW had no cardiac, hepatic, renal, or intestinal toxicity in mice. Alcoholic liver injury and steatosis in the NIAAA mode were effectively prevented by BGXJW treatment. BGXJW increased the expression of alcohol metabolizing enzymes ADH, CYP2E1, and ALDH2 to enhance alcohol metabolism, inhibited steatosis through regulating lipid metabolism, counteracted alcohol-induced upregulation of lipid synthesis related proteins SREBP1, FASN, and SCD1, meanwhile it enhanced fatty acids β-oxidation related proteins PPAR-α and CPT1A. Alcohol taken enhanced pro-inflammatory TNF-α, IL-6 and down-regulated the anti-inflammatory IL-10 expression in the liver, which were also reversed by BGXJW administration. Moreover, BGXJW significantly decreased the blood ethanol concentration and alleviated drunkenness in the alcohol binge-drinking mice model. Conclusions BGXJW could effectively relieve drunkenness and prevent alcoholic liver disease by regulating lipid metabolism, inflammatory response, and alcohol metabolism. Graphical Abstract |
| first_indexed | 2024-04-09T13:59:42Z |
| format | Article |
| id | doaj.art-26a54deccae6488598f5bcf5170630e2 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-04-09T13:59:42Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-26a54deccae6488598f5bcf5170630e22023-05-07T11:24:12ZengBMCChinese Medicine1749-85462023-05-0118111710.1186/s13020-023-00753-5A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice modelsHan Gao0Zhen Li1Yao Liu2Yong-kang Zhao3Cheng Cheng4Feng Qiu5Yuan Gao6Ya-wen Lu7Xin-hua Song8Jia-bo Wang9Zhi-tao Ma10College of Pharmacy, Fujian University of Traditional Chinese MedicineSchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversitySchool of Traditional Chinese Medicine, Capital Medical UniversityCollege of Pharmacy, Fujian University of Traditional Chinese MedicineSchool of Traditional Chinese Medicine, Capital Medical UniversityAbstract Background Bao-Gan-Xing-Jiu-Wan (BGXJW) is a clinical experience-based Chinese herbal formula. Its efficacy, pharmacological safety, targeted function, process quality, and other aspects have met the evaluation standards and the latest requirements of preparations. It could prevent and alleviate the symptoms of drunkenness and alcoholic liver injury clinically. The present work aims to elucidate whether BGXJW could protect against drunkenness and alcoholic liver disease in mice and explore the associated mechanism. Material and methods We used acute-on-chronic (NIAAA) mice model to induce alcoholic steatosis, and alcohol binge-drinking model to reappear the drunk condition. BGXJW at indicated doses were administered by oral gavage respectively to analyze its effects on alcoholic liver injury and the associated molecular mechanisms. Results BGXJW had no cardiac, hepatic, renal, or intestinal toxicity in mice. Alcoholic liver injury and steatosis in the NIAAA mode were effectively prevented by BGXJW treatment. BGXJW increased the expression of alcohol metabolizing enzymes ADH, CYP2E1, and ALDH2 to enhance alcohol metabolism, inhibited steatosis through regulating lipid metabolism, counteracted alcohol-induced upregulation of lipid synthesis related proteins SREBP1, FASN, and SCD1, meanwhile it enhanced fatty acids β-oxidation related proteins PPAR-α and CPT1A. Alcohol taken enhanced pro-inflammatory TNF-α, IL-6 and down-regulated the anti-inflammatory IL-10 expression in the liver, which were also reversed by BGXJW administration. Moreover, BGXJW significantly decreased the blood ethanol concentration and alleviated drunkenness in the alcohol binge-drinking mice model. Conclusions BGXJW could effectively relieve drunkenness and prevent alcoholic liver disease by regulating lipid metabolism, inflammatory response, and alcohol metabolism. Graphical Abstracthttps://doi.org/10.1186/s13020-023-00753-5Traditional Chinese medicineAlcoholic liver diseaseSteatosisInflammationEthanol metabolismDrunken behavior |
| spellingShingle | Han Gao Zhen Li Yao Liu Yong-kang Zhao Cheng Cheng Feng Qiu Yuan Gao Ya-wen Lu Xin-hua Song Jia-bo Wang Zhi-tao Ma A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models Chinese Medicine Traditional Chinese medicine Alcoholic liver disease Steatosis Inflammation Ethanol metabolism Drunken behavior |
| title | A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models |
| title_full | A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models |
| title_fullStr | A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models |
| title_full_unstemmed | A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models |
| title_short | A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models |
| title_sort | clinical experience based chinese herbal formula improves ethanol induced drunken behavior and hepatic steatohepatitis in mice models |
| topic | Traditional Chinese medicine Alcoholic liver disease Steatosis Inflammation Ethanol metabolism Drunken behavior |
| url | https://doi.org/10.1186/s13020-023-00753-5 |
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