The impact of host diet on Wolbachia titer in Drosophila.
While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose-...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-03-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4380406?pdf=render |
| _version_ | 1828378235466940416 |
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| author | Laura R Serbus Pamela M White Jessica Pintado Silva Amanda Rabe Luis Teixeira Roger Albertson William Sullivan |
| author_facet | Laura R Serbus Pamela M White Jessica Pintado Silva Amanda Rabe Luis Teixeira Roger Albertson William Sullivan |
| author_sort | Laura R Serbus |
| collection | DOAJ |
| description | While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer. |
| first_indexed | 2024-04-14T08:23:54Z |
| format | Article |
| id | doaj.art-26a774a62d564f8c861a05314ada20c2 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-14T08:23:54Z |
| publishDate | 2015-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-26a774a62d564f8c861a05314ada20c22022-12-22T02:04:08ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742015-03-01113e100477710.1371/journal.ppat.1004777The impact of host diet on Wolbachia titer in Drosophila.Laura R SerbusPamela M WhiteJessica Pintado SilvaAmanda RabeLuis TeixeiraRoger AlbertsonWilliam SullivanWhile a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.http://europepmc.org/articles/PMC4380406?pdf=render |
| spellingShingle | Laura R Serbus Pamela M White Jessica Pintado Silva Amanda Rabe Luis Teixeira Roger Albertson William Sullivan The impact of host diet on Wolbachia titer in Drosophila. PLoS Pathogens |
| title | The impact of host diet on Wolbachia titer in Drosophila. |
| title_full | The impact of host diet on Wolbachia titer in Drosophila. |
| title_fullStr | The impact of host diet on Wolbachia titer in Drosophila. |
| title_full_unstemmed | The impact of host diet on Wolbachia titer in Drosophila. |
| title_short | The impact of host diet on Wolbachia titer in Drosophila. |
| title_sort | impact of host diet on wolbachia titer in drosophila |
| url | http://europepmc.org/articles/PMC4380406?pdf=render |
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