Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium
Abstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various ind...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-022-00356-x |
| _version_ | 1797203414862528512 |
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| author | P. Rios B. Bezus S. Cavalitto I. Cavello |
| author_facet | P. Rios B. Bezus S. Cavalitto I. Cavello |
| author_sort | P. Rios |
| collection | DOAJ |
| description | Abstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h−1). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml−1) and keratinolytic (33.04 ± 1.09 U ml−1) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive. |
| first_indexed | 2024-04-24T08:18:58Z |
| format | Article |
| id | doaj.art-26aad25c69774e0f9e1d4977f5a42c81 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:18:58Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-26aad25c69774e0f9e1d4977f5a42c812024-04-17T01:58:49ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-05-0120111410.1186/s43141-022-00356-xProduction and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacteriumP. Rios0B. Bezus1S. Cavalitto2I. Cavello3Centro de Investigación y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas (CINDEFI, CCT La Plata-CONICET, UNLP), Universidad Nacional de la PlataCentro de Investigación y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas (CINDEFI, CCT La Plata-CONICET, UNLP), Universidad Nacional de la PlataCentro de Investigación y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas (CINDEFI, CCT La Plata-CONICET, UNLP), Universidad Nacional de la PlataCentro de Investigación y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas (CINDEFI, CCT La Plata-CONICET, UNLP), Universidad Nacional de la PlataAbstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h−1). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml−1) and keratinolytic (33.04 ± 1.09 U ml−1) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive.https://doi.org/10.1186/s43141-022-00356-xCold active keratinasePedobacterKeratin biodegradation |
| spellingShingle | P. Rios B. Bezus S. Cavalitto I. Cavello Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium Journal of Genetic Engineering and Biotechnology Cold active keratinase Pedobacter Keratin biodegradation |
| title | Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
| title_full | Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
| title_fullStr | Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
| title_full_unstemmed | Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
| title_short | Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
| title_sort | production and characterization of a new detergent stable keratinase expressed by pedobacter sp 3 14 7 a novel antarctic psychrotolerant keratin degrading bacterium |
| topic | Cold active keratinase Pedobacter Keratin biodegradation |
| url | https://doi.org/10.1186/s43141-022-00356-x |
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