Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices
Activin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulator...
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| Formato: | Artigo |
| Idioma: | English |
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Frontiers Media S.A.
2021-05-01
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| Colecção: | Frontiers in Cell and Developmental Biology |
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| Acesso em linha: | https://www.frontiersin.org/articles/10.3389/fcell.2021.660316/full |
| _version_ | 1831537578509074432 |
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| author | Lingling Jiang Lingling Jiang Yan Qi Xianghan Kong Runnan Wang Jianfei Qi Francis Lin Xueling Cui Zhonghui Liu |
| author_facet | Lingling Jiang Lingling Jiang Yan Qi Xianghan Kong Runnan Wang Jianfei Qi Francis Lin Xueling Cui Zhonghui Liu |
| author_sort | Lingling Jiang |
| collection | DOAJ |
| description | Activin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulatory effects on adhesion and migration of fibroblasts. We found that activin A induced the migration of fibroblast cell line L929 cells in transwell chamber and microfluidic device. Activin A also promoted L929 cells adhesion, but did not affect L929 cells viability or proliferation. In addition, activin A induced α-SMA expression and TGF-β1 release, which were factors closely related to tissue fibrosis, but had no effect on IL-6 production, a pro-inflammatory cytokine. Furthermore, activin A elevated calcium levels in L929 cells and increased p-ERK protein levels. Activin A-induced migration of L929 cells was attenuated by ERK inhibitor FR180204. To conclude, these data indicated that activin A as a novel chemokine induced the chemotactic migration of L929 cells via ERK signaling and possessed the pro-fibrosis role. These findings provide a new insight into understanding of activin A in tissue fibrosis. |
| first_indexed | 2024-12-16T23:10:11Z |
| format | Article |
| id | doaj.art-26aae5b9da31460eb1e726c421e2f988 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-16T23:10:11Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-26aae5b9da31460eb1e726c421e2f9882022-12-21T22:12:27ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-05-01910.3389/fcell.2021.660316660316Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic DevicesLingling Jiang0Lingling Jiang1Yan Qi2Xianghan Kong3Runnan Wang4Jianfei Qi5Francis Lin6Xueling Cui7Zhonghui Liu8Department of Oral Comprehensive Therapy, School and Hospital of Stomatology, Jilin University, Changchun, ChinaDepartment of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD, United StatesDepartment of Physics and Astronomy, University of Manitoba, Winnipeg, MB, CanadaDepartment of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaActivin A, a member of the transforming growth factor-beta (TGF-β) superfamily, contributes to tissue healing and fibrosis. As the innate tissue cells, fibroblasts also play an important role in wound healing and fibrosis. Herein, this study was aimed to investigate how activin A exhibited regulatory effects on adhesion and migration of fibroblasts. We found that activin A induced the migration of fibroblast cell line L929 cells in transwell chamber and microfluidic device. Activin A also promoted L929 cells adhesion, but did not affect L929 cells viability or proliferation. In addition, activin A induced α-SMA expression and TGF-β1 release, which were factors closely related to tissue fibrosis, but had no effect on IL-6 production, a pro-inflammatory cytokine. Furthermore, activin A elevated calcium levels in L929 cells and increased p-ERK protein levels. Activin A-induced migration of L929 cells was attenuated by ERK inhibitor FR180204. To conclude, these data indicated that activin A as a novel chemokine induced the chemotactic migration of L929 cells via ERK signaling and possessed the pro-fibrosis role. These findings provide a new insight into understanding of activin A in tissue fibrosis.https://www.frontiersin.org/articles/10.3389/fcell.2021.660316/fullactivin AfibroblastsmigrationERKmicrofluidic device |
| spellingShingle | Lingling Jiang Lingling Jiang Yan Qi Xianghan Kong Runnan Wang Jianfei Qi Francis Lin Xueling Cui Zhonghui Liu Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices Frontiers in Cell and Developmental Biology activin A fibroblasts migration ERK microfluidic device |
| title | Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices |
| title_full | Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices |
| title_fullStr | Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices |
| title_full_unstemmed | Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices |
| title_short | Activin A as a Novel Chemokine Induces Migration of L929 Fibroblasts by ERK Signaling in Microfluidic Devices |
| title_sort | activin a as a novel chemokine induces migration of l929 fibroblasts by erk signaling in microfluidic devices |
| topic | activin A fibroblasts migration ERK microfluidic device |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.660316/full |
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