Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence micros...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/1/35 |
| _version_ | 1797499346607931392 |
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| author | Shane M. Hickey Ben Ung Christie Bader Robert Brooks Joanna Lazniewska Ian R. D. Johnson Alexandra Sorvina Jessica Logan Carmela Martini Courtney R. Moore Litsa Karageorgos Martin J. Sweetman Douglas A. Brooks |
| author_facet | Shane M. Hickey Ben Ung Christie Bader Robert Brooks Joanna Lazniewska Ian R. D. Johnson Alexandra Sorvina Jessica Logan Carmela Martini Courtney R. Moore Litsa Karageorgos Martin J. Sweetman Douglas A. Brooks |
| author_sort | Shane M. Hickey |
| collection | DOAJ |
| description | Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology. |
| first_indexed | 2024-03-10T03:46:07Z |
| format | Article |
| id | doaj.art-26b29595c70c47f8a2ea463c7848b882 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:46:07Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-26b29595c70c47f8a2ea463c7848b8822023-11-23T11:19:11ZengMDPI AGCells2073-44092021-12-011113510.3390/cells11010035Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore ConsiderationsShane M. Hickey0Ben Ung1Christie Bader2Robert Brooks3Joanna Lazniewska4Ian R. D. Johnson5Alexandra Sorvina6Jessica Logan7Carmela Martini8Courtney R. Moore9Litsa Karageorgos10Martin J. Sweetman11Douglas A. Brooks12Clinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaClinical and Health Sciences, University of South Australia, Adelaide 5000, AustraliaFluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.https://www.mdpi.com/2073-4409/11/1/35fluorescence microscopymicroscopy techniquesimaging agentscellular imaging |
| spellingShingle | Shane M. Hickey Ben Ung Christie Bader Robert Brooks Joanna Lazniewska Ian R. D. Johnson Alexandra Sorvina Jessica Logan Carmela Martini Courtney R. Moore Litsa Karageorgos Martin J. Sweetman Douglas A. Brooks Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations Cells fluorescence microscopy microscopy techniques imaging agents cellular imaging |
| title | Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations |
| title_full | Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations |
| title_fullStr | Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations |
| title_full_unstemmed | Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations |
| title_short | Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations |
| title_sort | fluorescence microscopy an outline of hardware biological handling and fluorophore considerations |
| topic | fluorescence microscopy microscopy techniques imaging agents cellular imaging |
| url | https://www.mdpi.com/2073-4409/11/1/35 |
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