Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway
Selenium (Se)-enriched proteins are an important dietary source of Se for humans; however, only a few Se-enriched proteins have been identified. In the present study, we tested for potential antioxidant activity by Se-enriched soy protein, both in vitro and in vivo. Se-enriched soy protein isolate (...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2542 |
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| author | Xiaoli Zhao Jinyan Gao Astrid Hogenkamp Leon M. J. Knippels Johan Garssen Jing Bai Anshu Yang Yong Wu Hongbing Chen |
| author_facet | Xiaoli Zhao Jinyan Gao Astrid Hogenkamp Leon M. J. Knippels Johan Garssen Jing Bai Anshu Yang Yong Wu Hongbing Chen |
| author_sort | Xiaoli Zhao |
| collection | DOAJ |
| description | Selenium (Se)-enriched proteins are an important dietary source of Se for humans; however, only a few Se-enriched proteins have been identified. In the present study, we tested for potential antioxidant activity by Se-enriched soy protein, both in vitro and in vivo. Se-enriched soy protein isolate (S-SPI) was shown to have a higher free radical scavenging ability compared to ordinary soy protein isolate (O-SPI). Furthermore, Caco-2 cell viability was improved by S-SPI at low doses, whereas O-SPI did not. In addition, S-SPI was shown to inhibit oxidative stress via modulation of the NRF2-HO1 signaling pathway, upregulating the expression of downstream antioxidant enzymes (GPx, SOD). To further study the antioxidant capacity of S-SPI, BALB/c female mice were given oral gavages with 0.8 mL of S-SPI or O-SPI (5 g/kg/d, 20 g/kg/d and 40 g/kg/d) or saline as control. Hepatic GPx and SOD activity increased with increasing S-SPI dosage, but not with O-SPI. Taken together, our results suggest that Se-enriched soy protein has a high antioxidant ability and may be used as a dietary supplement for people with oxidative dam-age-mediated diseases. |
| first_indexed | 2024-03-10T05:30:14Z |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:30:14Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-26b457ecf7114a1e8dfdff5720e49feb2023-11-22T23:18:26ZengMDPI AGFoods2304-81582021-10-011011254210.3390/foods10112542Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling PathwayXiaoli Zhao0Jinyan Gao1Astrid Hogenkamp2Leon M. J. Knippels3Johan Garssen4Jing Bai5Anshu Yang6Yong Wu7Hongbing Chen8State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, 3584 CG Utrecht, The NetherlandsState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaSelenium (Se)-enriched proteins are an important dietary source of Se for humans; however, only a few Se-enriched proteins have been identified. In the present study, we tested for potential antioxidant activity by Se-enriched soy protein, both in vitro and in vivo. Se-enriched soy protein isolate (S-SPI) was shown to have a higher free radical scavenging ability compared to ordinary soy protein isolate (O-SPI). Furthermore, Caco-2 cell viability was improved by S-SPI at low doses, whereas O-SPI did not. In addition, S-SPI was shown to inhibit oxidative stress via modulation of the NRF2-HO1 signaling pathway, upregulating the expression of downstream antioxidant enzymes (GPx, SOD). To further study the antioxidant capacity of S-SPI, BALB/c female mice were given oral gavages with 0.8 mL of S-SPI or O-SPI (5 g/kg/d, 20 g/kg/d and 40 g/kg/d) or saline as control. Hepatic GPx and SOD activity increased with increasing S-SPI dosage, but not with O-SPI. Taken together, our results suggest that Se-enriched soy protein has a high antioxidant ability and may be used as a dietary supplement for people with oxidative dam-age-mediated diseases.https://www.mdpi.com/2304-8158/10/11/2542SeSe-enriched soy proteinantioxidantNRF2antioxidant enzyme |
| spellingShingle | Xiaoli Zhao Jinyan Gao Astrid Hogenkamp Leon M. J. Knippels Johan Garssen Jing Bai Anshu Yang Yong Wu Hongbing Chen Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway Foods Se Se-enriched soy protein antioxidant NRF2 antioxidant enzyme |
| title | Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway |
| title_full | Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway |
| title_fullStr | Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway |
| title_full_unstemmed | Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway |
| title_short | Selenium-Enriched Soy Protein Has Antioxidant Potential via Modulation of the NRF2-HO1 Signaling Pathway |
| title_sort | selenium enriched soy protein has antioxidant potential via modulation of the nrf2 ho1 signaling pathway |
| topic | Se Se-enriched soy protein antioxidant NRF2 antioxidant enzyme |
| url | https://www.mdpi.com/2304-8158/10/11/2542 |
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