Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers
Abstract Relaxin-2 exerts many favourable cardiovascular effects in pathological circumstances such as atrial fibrillation (AF) and heart failure, but the mechanisms underlying its actions are not completely understood. Since inflammation and fibrosis are pivotal processes in the pathogenesis of AF,...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26836-1 |
| _version_ | 1797977456975544320 |
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| author | Alana Aragón-Herrera Marinela Couselo-Seijas Sandra Feijóo-Bandín Laura Anido-Varela Sandra Moraña-Fernández Estefanía Tarazón Esther Roselló-Lletí Manuel Portolés José Luis Martínez-Sande Javier García-Seara Ezequiel Álvarez José Ramón González-Juanatey Moisés Rodríguez-Mañero Sonia Eiras Francisca Lago |
| author_facet | Alana Aragón-Herrera Marinela Couselo-Seijas Sandra Feijóo-Bandín Laura Anido-Varela Sandra Moraña-Fernández Estefanía Tarazón Esther Roselló-Lletí Manuel Portolés José Luis Martínez-Sande Javier García-Seara Ezequiel Álvarez José Ramón González-Juanatey Moisés Rodríguez-Mañero Sonia Eiras Francisca Lago |
| author_sort | Alana Aragón-Herrera |
| collection | DOAJ |
| description | Abstract Relaxin-2 exerts many favourable cardiovascular effects in pathological circumstances such as atrial fibrillation (AF) and heart failure, but the mechanisms underlying its actions are not completely understood. Since inflammation and fibrosis are pivotal processes in the pathogenesis of AF, our aim was to study the relationship between relaxin-2 plasma levels in left atrium (LA) and peripheral vein with molecules implicated in fibrosis, inflammation and oxidative stress in AF patients, and to evaluate the anti-fibrotic ability of relaxin-2 in normal human atrial cardiac fibroblasts (NHCF-A). Peripheral vein relaxin-2 plasma levels were higher than LA relaxin-2 plasma levels in men while, in women, peripheral vein relaxin-2 levels were increased compared to men. AF patients with higher levels of relaxin-2 exhibited a reduction in H2O2 plasma levels and in mRNA levels of alpha-defensin 3 (DEFA3) and IL-6 in leucocytes from LA plasma. Relaxin-2-in-vitro treatment inhibited NHCF-A migration and decreased mRNA and protein levels of the pro-fibrotic molecule transforming growth factor-β1 (TGF-β1). Our results support an association between relaxin-2 and molecules involved in fibrosis, inflammation and oxidative stress in AF patients, and reinforce an anti-fibrotic protective role of this hormone in NHCF-A; strengthening the relevance of relaxin-2 in AF physiopathology, diagnosis and treatment. |
| first_indexed | 2024-04-11T05:07:11Z |
| format | Article |
| id | doaj.art-26b7187489d244cd8003e835c103ccd1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:07:11Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-26b7187489d244cd8003e835c103ccd12022-12-25T12:15:07ZengNature PortfolioScientific Reports2045-23222022-12-0112111510.1038/s41598-022-26836-1Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markersAlana Aragón-Herrera0Marinela Couselo-Seijas1Sandra Feijóo-Bandín2Laura Anido-Varela3Sandra Moraña-Fernández4Estefanía Tarazón5Esther Roselló-Lletí6Manuel Portolés7José Luis Martínez-Sande8Javier García-Seara9Ezequiel Álvarez10José Ramón González-Juanatey11Moisés Rodríguez-Mañero12Sonia Eiras13Francisca Lago14Cellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)Translational Cardiology Group, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)Cellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)Cellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)Cellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)CIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)CIBERCV, Institute of Health Carlos IIICIBERCV, Institute of Health Carlos IIICellular and Molecular Cardiology Unit and Department of Cardiology, Institute of Biomedical Research of Santiago de Compostela (IDIS-SERGAS)Abstract Relaxin-2 exerts many favourable cardiovascular effects in pathological circumstances such as atrial fibrillation (AF) and heart failure, but the mechanisms underlying its actions are not completely understood. Since inflammation and fibrosis are pivotal processes in the pathogenesis of AF, our aim was to study the relationship between relaxin-2 plasma levels in left atrium (LA) and peripheral vein with molecules implicated in fibrosis, inflammation and oxidative stress in AF patients, and to evaluate the anti-fibrotic ability of relaxin-2 in normal human atrial cardiac fibroblasts (NHCF-A). Peripheral vein relaxin-2 plasma levels were higher than LA relaxin-2 plasma levels in men while, in women, peripheral vein relaxin-2 levels were increased compared to men. AF patients with higher levels of relaxin-2 exhibited a reduction in H2O2 plasma levels and in mRNA levels of alpha-defensin 3 (DEFA3) and IL-6 in leucocytes from LA plasma. Relaxin-2-in-vitro treatment inhibited NHCF-A migration and decreased mRNA and protein levels of the pro-fibrotic molecule transforming growth factor-β1 (TGF-β1). Our results support an association between relaxin-2 and molecules involved in fibrosis, inflammation and oxidative stress in AF patients, and reinforce an anti-fibrotic protective role of this hormone in NHCF-A; strengthening the relevance of relaxin-2 in AF physiopathology, diagnosis and treatment.https://doi.org/10.1038/s41598-022-26836-1 |
| spellingShingle | Alana Aragón-Herrera Marinela Couselo-Seijas Sandra Feijóo-Bandín Laura Anido-Varela Sandra Moraña-Fernández Estefanía Tarazón Esther Roselló-Lletí Manuel Portolés José Luis Martínez-Sande Javier García-Seara Ezequiel Álvarez José Ramón González-Juanatey Moisés Rodríguez-Mañero Sonia Eiras Francisca Lago Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers Scientific Reports |
| title | Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| title_full | Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| title_fullStr | Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| title_full_unstemmed | Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| title_short | Relaxin-2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| title_sort | relaxin 2 plasma levels in atrial fibrillation are linked to inflammation and oxidative stress markers |
| url | https://doi.org/10.1038/s41598-022-26836-1 |
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