Phylogenetic tree analysis of Entamoeba species isolated from goats
The present study was designed to species typing of parasite Entamoeba spp from goats by using PCR technique and phylogenetic tree analysis. The PCR technique was conducted for using specific primers were designed for 18S rRNA gene of Entamoeba spp. In this study, the sequence alignment analysis and...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine
2017-12-01
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| Series: | Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| Online Access: | http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/441 |
| _version_ | 1818682458777845760 |
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| author | Nuha Qasim Mohammed |
| author_facet | Nuha Qasim Mohammed |
| author_sort | Nuha Qasim Mohammed |
| collection | DOAJ |
| description | The present study was designed to species typing of parasite Entamoeba spp from goats by using PCR technique and phylogenetic tree analysis. The PCR technique was conducted for using specific primers were designed for 18S rRNA gene of Entamoeba spp. In this study, the sequence alignment analysis and phylogenetic tree analysis of Unweighted Pair Group method with Arithmetic were performed by using phylogenetic and molecular evolutionary analysis (MEGA 6.0 edition computer software) that analysis of 590bp for ribosomal 18S rRNA gene. Our isolates submitted to the National Center for Biotechnology Information (NCBI-GenBank) for getting accession number and then we were gotten (10) accession number for goat isolates. Entamoeba spp were detected in (10/50) (20%) of feces samples that collected from goat by PCR. Results of the phylogenetic tree analysis show that most isolates of Entamoeba spp. were closed related to NCBI-Blast Entamoeba bovis 18S ribosomal RNA gene (FN666250.1) with (80%) as an accession number (MF568371, MF568372, MF568373, MF568374, MF568375, MF568377, MF568378, and MF568380), whereas other NCBI- Blast Entamoeba spp. has been shown more related to Entamoeba histolytica isolate 18S ribosomal RNA gene (GQ423749.1) with (20%) as (MF568376 and MF568379). This study is first recording used of molecular phylogeny to Entamoeba spp. in goat at the first time in Iraq. |
| first_indexed | 2024-12-17T10:19:10Z |
| format | Article |
| id | doaj.art-26b73ccda3f941a89531582b024cd3a5 |
| institution | Directory Open Access Journal |
| issn | 1818-5746 2313-4429 |
| language | English |
| last_indexed | 2024-12-17T10:19:10Z |
| publishDate | 2017-12-01 |
| publisher | College of Veterinary Medicine |
| record_format | Article |
| series | Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| spelling | doaj.art-26b73ccda3f941a89531582b024cd3a52022-12-21T21:52:50ZengCollege of Veterinary MedicineAl-Qadisiyah Journal of Veterinary Medicine Sciences1818-57462313-44292017-12-011623844Phylogenetic tree analysis of Entamoeba species isolated from goatsNuha Qasim Mohammed0Department of Microbiology and parasitology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.The present study was designed to species typing of parasite Entamoeba spp from goats by using PCR technique and phylogenetic tree analysis. The PCR technique was conducted for using specific primers were designed for 18S rRNA gene of Entamoeba spp. In this study, the sequence alignment analysis and phylogenetic tree analysis of Unweighted Pair Group method with Arithmetic were performed by using phylogenetic and molecular evolutionary analysis (MEGA 6.0 edition computer software) that analysis of 590bp for ribosomal 18S rRNA gene. Our isolates submitted to the National Center for Biotechnology Information (NCBI-GenBank) for getting accession number and then we were gotten (10) accession number for goat isolates. Entamoeba spp were detected in (10/50) (20%) of feces samples that collected from goat by PCR. Results of the phylogenetic tree analysis show that most isolates of Entamoeba spp. were closed related to NCBI-Blast Entamoeba bovis 18S ribosomal RNA gene (FN666250.1) with (80%) as an accession number (MF568371, MF568372, MF568373, MF568374, MF568375, MF568377, MF568378, and MF568380), whereas other NCBI- Blast Entamoeba spp. has been shown more related to Entamoeba histolytica isolate 18S ribosomal RNA gene (GQ423749.1) with (20%) as (MF568376 and MF568379). This study is first recording used of molecular phylogeny to Entamoeba spp. in goat at the first time in Iraq.http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/441 |
| spellingShingle | Nuha Qasim Mohammed Phylogenetic tree analysis of Entamoeba species isolated from goats Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| title | Phylogenetic tree analysis of Entamoeba species isolated from goats |
| title_full | Phylogenetic tree analysis of Entamoeba species isolated from goats |
| title_fullStr | Phylogenetic tree analysis of Entamoeba species isolated from goats |
| title_full_unstemmed | Phylogenetic tree analysis of Entamoeba species isolated from goats |
| title_short | Phylogenetic tree analysis of Entamoeba species isolated from goats |
| title_sort | phylogenetic tree analysis of entamoeba species isolated from goats |
| url | http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/441 |
| work_keys_str_mv | AT nuhaqasimmohammed phylogenetictreeanalysisofentamoebaspeciesisolatedfromgoats |