Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line
Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2019-12-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/294 |
| _version_ | 1811232094236442624 |
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| author | Roihatul Mutiah Trian Sidha Minggarwati Risma Aprinda Kristanti Erna Susanti |
| author_facet | Roihatul Mutiah Trian Sidha Minggarwati Risma Aprinda Kristanti Erna Susanti |
| author_sort | Roihatul Mutiah |
| collection | DOAJ |
| description | Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).
Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer |
| first_indexed | 2024-04-12T10:57:36Z |
| format | Article |
| id | doaj.art-26b7ed1267044f07a4f34c29fde743ba |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-12T10:57:36Z |
| publishDate | 2019-12-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-26b7ed1267044f07a4f34c29fde743ba2022-12-22T03:36:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892019-12-0110313113910.14499/indonesianjcanchemoprev10iss3pp131-139190Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell LineRoihatul Mutiah0Trian Sidha Minggarwati1Risma Aprinda Kristanti2Erna Susanti3Department of Pharmacy, Faculty of Medicine and Health Sciences, Maulana Malik Ibrahim State Islamic University, MalangDepartment of Pharmacy, Faculty of Medicine and Health Sciences, Maulana Malik Ibrahim State Islamic University, MalangDepartment of Medical Education, Faculty of Medicine and Health Sciences, Maulana Malik Ibrahim State Islamic University, MalangPharmacy and Food Analysis, Academy Putera Indonesia, MalangEleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical). Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancerhttps://ijcc.chemoprev.org/index.php/ijcc/article/view/294 |
| spellingShingle | Roihatul Mutiah Trian Sidha Minggarwati Risma Aprinda Kristanti Erna Susanti Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| title_full | Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| title_fullStr | Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| title_full_unstemmed | Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| title_short | Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line |
| title_sort | compound identification and anticancer activity of ethyl acetate fraction from bawang sabrang eleutherine palmifolia l merr on hela cervical cancer cell line |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/294 |
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