Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation
There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1366 |
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| author | Yujia Xu Hongwei Zheng Jianxin Sui Hong Lin Limin Cao |
| author_facet | Yujia Xu Hongwei Zheng Jianxin Sui Hong Lin Limin Cao |
| author_sort | Yujia Xu |
| collection | DOAJ |
| description | There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of <i>Staphylococcus aureus</i> was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for <i>S. aureus</i> was evaluated as 1.87 × 10<sup>8</sup> CFU/cm<sup>2</sup>, the linear range of the fluorescent analysis was 10<sup>4</sup> CFU/mL~10<sup>8</sup> CFU/mL (R<sup>2</sup> = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 10<sup>2</sup> CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens. |
| first_indexed | 2024-03-11T05:37:32Z |
| format | Article |
| id | doaj.art-26b94366301c48d8b0b049b4bcd53f41 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:37:32Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-26b94366301c48d8b0b049b4bcd53f412023-11-17T16:40:18ZengMDPI AGFoods2304-81582023-03-01127136610.3390/foods12071366Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity IsolationYujia Xu0Hongwei Zheng1Jianxin Sui2Hong Lin3Limin Cao4Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaKey Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266100, ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266100, ChinaThere are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of <i>Staphylococcus aureus</i> was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for <i>S. aureus</i> was evaluated as 1.87 × 10<sup>8</sup> CFU/cm<sup>2</sup>, the linear range of the fluorescent analysis was 10<sup>4</sup> CFU/mL~10<sup>8</sup> CFU/mL (R<sup>2</sup> = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 10<sup>2</sup> CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens.https://www.mdpi.com/2304-8158/12/7/1366boronate affinity<i>Staphylococcus aureus</i>enrichmentfluorescent probes |
| spellingShingle | Yujia Xu Hongwei Zheng Jianxin Sui Hong Lin Limin Cao Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation Foods boronate affinity <i>Staphylococcus aureus</i> enrichment fluorescent probes |
| title | Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation |
| title_full | Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation |
| title_fullStr | Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation |
| title_full_unstemmed | Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation |
| title_short | Rapid and Sensitive Fluorescence Detection of <i>Staphylococcus aureus</i> Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation |
| title_sort | rapid and sensitive fluorescence detection of i staphylococcus aureus i based on polyethyleneimine enhanced boronate affinity isolation |
| topic | boronate affinity <i>Staphylococcus aureus</i> enrichment fluorescent probes |
| url | https://www.mdpi.com/2304-8158/12/7/1366 |
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